1887

Abstract

Summary

This report describes a PCR primer pair that targets the D GDP mannose gene of and produces a specific 520-bp PCR product useful for identification. This PCR assay was tested with 182 isolates of and 20 isolates of other bacterial species, and demonstrated 100% specificity and sensitivity. The test was also able to detect directly in clinical samples such as sputum or throat swabs obtained from cystic fibrosis patients. The combination of this primer with a universal bacterial primer, acting as a control to assess DNA quality in the sample, resulted in a robust PCR method that can be used for rapid detection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-48-4-357
1999-04-01
2019-10-15
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-48-4-357
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error