Clonal analysis based on ribotyping demonstrated that strains isolated mainly from diarrhoeal stools in Sāo Paulo, Brazil, were clustered into two main groups. Eleven distinct ribotype patterns were identified with I, RV and I restriction endonucleases. strains with invasive properties were of two ribotype patterns that differed from those identified among non-invasive strains. The ribotyping results confirmed that strains associated with diarrhoeal disease in Sāo Paulo represent distinct groups of strains. Although the invasive strains were isolated from different patients over an extended period they were clustered into two genetically related clones, which seemed to be distributed endemically in the population studied.


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