Of 37 isolates from various aquatic environments, seven were and 30 These isolates were biotyped, serotyped and tested for their susceptibility to 20 antibiotics. All isolates were of biovar 1; those of were distributed amongst four biovars (1, 2, 4 and 6). On the basis of combined biotyping and serotyping results, isolates were distributed in five and in 17 groups. With the exception of one isolate which was resistant to tetracycline and streptomycin, the isolates were sensitive to the non-β-lactam antibiotics. In contrast, various patterns of β-lactam insensitivity were detected, including ampicillin and ticarcillin (35 isolates), cephalothin (33 isolates), carbenicillin (32 isolates), amoxycillin/clavulanate (23 isolates) and cefoxitin (22 isolates). No correlation between biotype or serotype and the susceptibility pattern of the isolates was apparent. Both inducible cephalosporinase activity against third-generation cephalosporins and inhibition of resistance to penicillins were detected in all and isolates by double-disk tests.


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