A series of 35 isolates of from 29 subjects (five AIDS patients and 24 HIV-seronegative individuals) was typed by electrophoretic karyotyping (EK), restriction fragment length polymorphism (RFLP) analysis, random amplification of polymorphic DNA (RAPD) and inter-repeat PCR (IR-PCR). The rank order of discriminatory ability among the four methods was as follows: EK (25 DNA types) > RAPD (19 DNA types) > IR-PCR (14 DNA types) > RFLP (4 DNA types). A composite DNA type was defined for each of the strains as the combination of types obtained by the four molecular methods. A total of 32 DNA types was obtained by this procedure; each individual harboured their own specific isolate (DNA type). Neither source of isolation nor HIV status was associated with a given DNA type. In three of five cases, initial and relapse isolates from individual patients were assigned to the same DNA type. These findings indicate that EK is the most useful method for the investigation of inter-strain variations within this species.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error