1887

Abstract

Summary

A method for removing inhibitor(s) of the PCR assay for the direct detection of cholera toxin A gene () in human faeces is described. Inhibitors of the PCR were removed by centrifugation and the activity of the remaining inhibitors by dilution. Based on these data, a protocol was developed for pre-treatment of stool specimens for PCR assay, and a simple and rapid protocol was constructed for the diagnostic detection of the genes in stool specimens in combination with single band detection on gel electrophoresis, dot-blot hybridisation and enrichment culture. This protocol was applied to clinical specimens and showed that the PCR method gave 100% agreement with established culture methods for the detection of cholera toxin-producing O1. This protocol was considered to be useful because of its simplicity and the rapidity of diagnosis.

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/content/journal/jmm/10.1099/00222615-48-10-883
1999-10-01
2019-11-21
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-48-10-883
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