Application of molecular typing methods to dermatophyte species that cause skin and nail infections Free

Abstract

Typing methods utilising DNA technology were applied to a collection of and isolates from skin and nail infections. The methods included restriction enzyme analysis (REA), hybridisation with the DNA probe poly (dG-dT), randomly amplified polymorphic DNA (RAPD) by PCR and restriction analysis of a segment of PCR-amplified rDNA. All these tests successfully differentiated the species, but few intra-species differences were detected. REA demonstrated some isolate variation, but this was limited and difficult to interpret, making it unsuitable as a typing tool. RAPD demonstrated few variations amongst and none in

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/content/journal/jmm/10.1099/00222615-48-1-33
1999-01-01
2024-03-29
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-48-1-33
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