1887

Abstract

Iron uptake analysis suggested that the transferrin (Tf) binding proteins, TbpA and TbpB, form only one type of receptor complex. Mutants defective in the synthesis of either TbpA or TbpB, but not defective in both proteins, can bind Tf, suggesting that both proteins are surface exposed and function in Tf binding. Also, iron uptake from Tf into the meningococci did not require the presence of both Tbps. The TbpB-defective mutant incorporated . 37% of the iron taken up by the wild-type strain, but this was insufficient for bacterial growth. The TbpA-defective mutant incorporated . 50% of the iron taken up by the wild-type strain and was able to grow with Tf as the only iron source. Mouse antibodies specific for TbpA were able to block . 70% of the iron uptake from Tf in the wild-type strain, whereas they blocked only 22% of iron uptake in the TbpB-defective mutant and did not block uptake in the TbpA-defective strain. These results emphasise that TbpA should be considered in future vaccine trials in which iron-restricted proteins are to be included in the vaccine formulation.

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1998-09-01
2024-12-07
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References

  1. Ala’Aldeen D. A. A., Stevenson P., Griffiths E. Immune responses in humans and animals to meningococcal transferrin-binding proteins: implications for vaccine design. Infect Immun 1994; 62:2984–2990
    [Google Scholar]
  2. Ferrón L., Ferreiros C. M., Criado M. T., Pintor M. Immunogenicity and antigenic heterogeneity of a human transferrin-binding protein in Neisseria meningitidis. Infect Immun 1992; 60:2887–2892
    [Google Scholar]
  3. Pintor M., Ferrón L., Gómez J. A., Gorringe A., Criado M. T., Ferreirós C. M. Blocking of iron uptake by monoclonal antibodies specific for the Neisseria meningitidis transferrinbinding protein 2. J Med Microbiol 1996; 45:252–257
    [Google Scholar]
  4. Ferrón L., Ferreirós C. M., Criado M. T., Andrade M. P. Purification of the Neisseria meningitidis transferrin binding protein-2 (TBP2) to homogeneity using column chromatography. FEMS Microbiol Lett 1993; 109:159–166
    [Google Scholar]
  5. Pintor M., Ferreirós C. M., Criado M. T. Characterization of the transferrin-iron uptake system in Neisseria meningitidis. FEMS Microbiol Lett 1993; 112:159–166
    [Google Scholar]
  6. Cornelissen C. N., Biswas G. D., Tsai J., Paruchuri D. K., Thompson S. A., Sparling P. F. Gonococcal transferrin-binding protein 1 is required for transferrin utilization and is homologous to TonB-dependent outer membrane receptors. J Bacteriol 1992; 174:5788–5797
    [Google Scholar]
  7. Anderson J. E., Sparling P. F., Cornelissen C. N. Gonococcal transferrin-binding protein 2 facilitates but is not essential for transferrin utilization. J Bacteriol 1994; 176:3162–3170
    [Google Scholar]
  8. Irwin S. W., Averil N., Cheng C. Y., Schryvers A. B. Preparation and analysis of isogenic mutants in the transferrin receptor protein genes, tbpA and tbpB, from Neisseria meningitidis. Mol Microbiol 1993; 8:1125–113
    [Google Scholar]
  9. Pintor M., Ferreirós C. M., Criado M. T. Energy-independent binding of iron complexed to small organic chelants by Neisseria meningitides. J Gen Appl Microbiol 1994; 40:23–34
    [Google Scholar]
  10. Pintor M., Ferreirós C. M., Criado M. T., Ferrón L. Expression levels of human transferrin receptors in Neisseria species. J Microbiol Methods 1992; 15:321–326
    [Google Scholar]
  11. Pintor M., Ferron L., Gomez J. A. Blocking of iron uptake from transferrin by antibodies against the transferrin binding proteins in Neisseria meningitidis. Microb Pathog 1996; 20:127–139
    [Google Scholar]
  12. Powell N. B. L., Bishop K., Gorringe A. R., Schryvers A. B. Co-localization of the meningococcal binding proteins (Tbp1 and Tbp2) and evaluation of their relative roles in binding human transferrin. In Zollinger W. D., Frasch C. E., Deal C. D. (eds) Abstracts of the Tenth International Pathogenic Neisseria Conference; Baltimore: 1996584
    [Google Scholar]
  13. Boulton I. C., Gorringe A. R., Allison N., Gomisky B., Evans R. W. Characterization of the interaction between Neisseria meningitidis transferrin binding proteins and transferrin by gel filtration and surface plasmon resonance. In Zollinger W. D., Frasch C. E., Deal C. D. (eds) Abstracts of the Tenth International Pathogenic Neisseria Conference; Baltimore: 1996562–563
    [Google Scholar]
  14. Comelissen C. N., Sparling P. F. Binding and surface exposure characteristics of the gonococcal transferrin receptor are dependent on both transferrin-binding proteins. J Bacteriol 1996; 178:1437–1444
    [Google Scholar]
  15. Danve B., Lissolo L., Mignon M. Transferrin-binding proteins isolated from Neisseria meningitidis elicit protective and bactericidal antibodies in laboratory animals. Vaccine 1993; 11:1214–1220
    [Google Scholar]
  16. Ala’Aldeen D. A. A., Borriello S. P. The meningococcal transferrin-binding proteins 1 and 2 are both surface exposed and generate bactericidal antibodies capable of killing homologous and heterologous strains. Vaccine 1996; 14:49–53
    [Google Scholar]
  17. Rokbi B., Mazarin V., Maitre-Wilmotte G., Quentin-Millet M.-J. Identification of two major families of transferrin receptors among Neisseria meningitidis strains based on antigenic and genomic features. FEMS Microbiol Lett 1993; 110:51–58.vn
    [Google Scholar]
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