1887

Abstract

is a micro-aerophilic urease-positive bacterium that has recently been isolated from the intestinal mucosa of rats. The purpose of this investigation was to study the ultrastructural details of this micro-organism in both pure culture and in the gastrointestinal tract of germ-free mice infected with . The micro-organism was a fusiform to slightly spiral gram-negative cell, 4–6 μm long and 0.6–0.7 μm wide, with four to seven bipolar sheathed flagella. The cytoplasm presented several irregular and also globular granules. On each side of the polar regions of the cells, there was a highly electron-dense band, the ‘polar membrane’. Coccoidal forms were seen in old cultures. showed several ultrastructural characteristics of the genus and much resemblance to and mainly colonised the large bowel of the gnotobiotic mice where it could be seen in the lumen and also inside the enterocytes. Vacuolation of the ileal epithelial cells, loss of microvilli and pronounced desquamation of the enterocytes of the caecum were observed in the bowel colonised by the bacterium. These observations raise the possibility that could cause some harm to the host at least in particular circumstances such as when it colonises the gastrointestinal tract of a germ-free host.

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1998-06-01
2024-12-14
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References

  1. Marshall B. J., Warren J. R. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984; 1:1311–1315
    [Google Scholar]
  2. Blaser M. J. Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis 1990; 161:626–633
    [Google Scholar]
  3. Graham D. Y., Go M. F. Helicobacter pylori: current status. Gastroenterology 1993; 105:279–282
    [Google Scholar]
  4. Parsonnet J., Friedman G. D., Vandersteen D. P. Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med 1991; 325:1127–1131
    [Google Scholar]
  5. Wotherspoon A. C., Doglioni C., Diss T. C. Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori. Lancet 1993; 342:575–577
    [Google Scholar]
  6. Lee A., Phillips M. W., O’Rourke J. L. Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. Int J Syst Bacteriol 1992; 42:27–36
    [Google Scholar]
  7. Fox J. G., Dewhirst F. E., Tully J. G. Helicobacter hepaticus sp. nov., a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice. J Clin Microbiol 1994; 32:1238–1245
    [Google Scholar]
  8. Fox J. G., Yan L. L., Dewhirst F. E. Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice. J Clin Microbiol 1995; 33:445–454
    [Google Scholar]
  9. Vandamme P., Falsen E., Rossau R. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol 1991; 41:88–103
    [Google Scholar]
  10. Franklin C. L., Beckwith C. S., Livingston R. S. Isolation of a novel Helicobacter species, Helicobacter cholecystus sp. nov., from the gallbladders of Syrian hamsters with cholangiofibrosis and centrilobular pancreatitis. J Clin Microbiol 1996; 34:2952–2958
    [Google Scholar]
  11. Mendes E. N., Queiroz D. M. M., Dewhirst F. E., Paster B. J., Moura S. B., Fox J. G. Helicobacter trogontum sp. nov., isolated from the rat intestine. Int J Syst Bacteriol 1996; 46:916–921
    [Google Scholar]
  12. Shen Z., Fox J. G., Dewhirst F. E. Helicobacter rodentium sp. nov., a urease-negative Helicobacter species isolated from laboratory mice. Int J Syst Bacteriol 1997; 47:627–634
    [Google Scholar]
  13. Gebhart C. J., Fennell C. L., Murtaugh M. P., Stamm W. E. Campylobacter cinaedi is normal intestinal flora in hamsters. J Clin Microbiol 1989; 27:1692–1694
    [Google Scholar]
  14. Schauer D. B., Ghori N., Falkow S. Isolation and characterization of “ Flexispira rappini” from laboratory mice. J Clin Microbiol 1993; 31:2709–2714
    [Google Scholar]
  15. Queiroz D. M. M., Contigli C., Coimbra R. S. Spiral bacterium associated with gastric, ileal and caecal mucosa of mice. Lab Anim 1992; 26:288–294
    [Google Scholar]
  16. Lee A., O’Rourke J. Ultrastructure of Helicobacter organisms and possible relevance for pathogenesis. In Goodwin C. S., Worsley B. W. (eds) Helicobacter pylori. Biology and clinical practice Boca Raton F. L.: CRC Press; 199315–36
    [Google Scholar]
  17. Bode G., Malfertheiner P., Ditschuneit H. Pathogenetic implications of ultrastructural findings in Campylobacter pylori related gastroduodenal disease. Scand J Gastroenterol 1988; 23: Suppl 14225–39
    [Google Scholar]
  18. Bode G., Mauch F., Ditschuneit H., Malfertheiner P. Identification of structures containing polyphosphate in Helicobacter pylori. J Gen Microbiol 1993; 139:3029–3033
    [Google Scholar]
  19. Brock T. D., Madigan M. T., Martinko J. M., Parker J. Cell biology. In Biology of microorganisms 7th edn New Jersey: Prentice Hall; 199443–88
    [Google Scholar]
  20. Lee A., O’Rourke J. Gastric bacteria other than Helicobacter pylori. Gastroenterol Clin North Am 1993; 22:21–42
    [Google Scholar]
  21. Zeller J., Takeuchi A. Infection of the colon of the rhesus monkey by spiral-shaped organisms. Vet Pathol 1982; 19: Suppl 726–32
    [Google Scholar]
  22. Dubois A., Fiala N., Heman-Ackah L. M. Natural gastric infection with Helicobacter pylori in monkeys: a model for spiral bacteria infection in humans. Gastroenterology 1994; 106:1405–1417
    [Google Scholar]
  23. Sherman P. M. Adherence and internalization of H. pylori by epithelial cells. In Hunt R. H., Tytgat G. N. J. (eds) Helicobacter pylori. Basic mechanisms to clinical cure Dordrecht: Kluwer Academic Publishers; 1993148–162
    [Google Scholar]
  24. O’Rourke J., Lee A., Fox J. G. An ultrastructural study of Helicobacter mustelae and evidence of a specific association with gastric mucosa. J Med Microbiol 1992; 36:420–427
    [Google Scholar]
  25. Phillips M. W., Lee A. Isolation and characterization of a spiral bacterium from the crypts of rodent gastrointestinal tracts. Appl Environ Microbiol 1983; 45:675–683
    [Google Scholar]
  26. Bode G., Malfertheiner P., Lehnhardt G., Ditschuneit H. Virulence factors of Helicobacter pylori-ultrastructural features. In Malfertheiner P., Ditschuneit H. (eds) Helicobacter pylori, gastritis and ulcer Berlin: Springer-Verlag; 199063–73
    [Google Scholar]
  27. Ogata T., Araki K. Electron microscopic study of the morphological changes of gastric mucus cell induced by Helicobacter pylori in human gastric ulcers. J Submicrosc Cytol Pathol 1996; 28:255–264
    [Google Scholar]
  28. Cover T. L., Cao P., Lind C. D., Tham K. T., Blaser M. Correlation between vacuolating cytotoxin production by Helicobacter pylori isolates in vitro and in vivo. Infect Immun 1993; 61:5008–5012
    [Google Scholar]
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