@article{mbs:/content/journal/jmm/10.1099/00222615-47-6-505, author = "Osaki, Takako and Yamaguchi, Hiroyuki and Taguchi, Haruhiko and Fukuda, Minoru and Kawakami, Hayato and Hirano, Hiroshi and Watanabe, Sumio and Takagi, Atsushi and Kamiya, Shigeru", title = "Establishment and characterisation of a monoclonal antibody to inhibit adhesion of Helicobacter pylori to gastric epithelial cells", journal= "Journal of Medical Microbiology", year = "1998", volume = "47", number = "6", pages = "505-512", doi = "https://doi.org/10.1099/00222615-47-6-505", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-47-6-505", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Monoclonal antibodies (MAbs) that inhibit adhesion of Helicobacter pylori to human gastric cancer (MKN45) cells were established to clarify the mechanism of adhesion of H. pylori. Of 53 hybridoma clones screened by the primary inhibition assay for adhesion, MAb A20 of IgM class was selected on the basis of both its reactivity to whole cells of H. pylori by ELISA and its inhibitory effect on adhesion of H. pylori. The adhesion of H. pylori strain TK1029 to MKN45 cells was inhibited by MAb A20, depending on the concentration of the MAb. The MAb recognised the surface antigen, lipopolysaccharide (LPS) of H. pylori, suggesting that LPS is associated with adhesion of H. pylori to human gastric epithelial cells.", }