In-vivo administration of the culture supernates from resulted in thymus atrophy in C3H/HeJ mice, known to be lipopolysaccharide (LPS)-non-responders. The thymocytes underwent apoptosis as characterised by fragmented DNA ladders on agarose gel electrophoresis, a cell death detection ELISA and a morphological study by the TUNEL reaction. As a control, LPS treatment did not induce thymocyte apoptosis in C3H/HeJ mice. Flow cytometric analysis indicated that thymus atrophy was due predominantly to the deletion of CD4CD8T cells. When cells were undergoing apoptosis, an elevation in the percentage of T-cell receptor (TCR)-αβ cells was observed at 24 h, which was correlated with the increase in the percentages of cells expressing high levels of the Vβ6 and Vβ8 TCR. Gel electrophoretic analysis demonstrated the presence of protein bands with mol. wts ranging from 17 to 65 kDa in culture supernates.


Article metrics loading...

Loading full text...

Full text loading...


Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error