Cell-bound haemolytic activity was observed in isolates of complex (MAC) from AIDS patients. type strains showed negligible activity. None of the culture supernates exhibited any haemolytic activity. Zwitterionic detergent 3-[(3-cholamidopropyl)-dimethyl-ammonio]-1-propanesulphonate (CHAPS) was used to extract haemolysin from ethanol-treated complex strain 101 (MAC101) cells. Haemolysin was isolated from CHAPS extract (CE) by metal affinity chromatography and identified as a 32-kDa protein by polyclonal antibodies raised against haemolysin. Treatment of CE with trypsin resulted in reduction of haemolytic activity, whereas heating at 100°C for 10 min did not affect its activity. A similar 32-kDa haemolysin was extracted from cells of K128 which was isolated from a monkey infected with simian immunodeficiency virus (SIV). The haemolysin produced by strains isolated from AIDS patients may be associated with the pathogenesis of infection.


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