1887

Abstract

Ultrastructural characteristics of 15 strains and isolates of ehrlichiae belonging to three genogroups, or clades of genetically related organisms united in the genera and a strain of which represents a fourth genogroup in this cluster of species, were studied in continuous cell culture or (Oklahoma strain and VHE isolate), (AS 145), (Arkansas, 91HE17 and Sapulpa), human granulocytic ehrlichiae (HGE)(BDS, 96HE27, 96HE37, #54, #55 and #72), (MRK), (Miyayama), (HRC-IL). was studied in the naturally infected mosquito cell line Aa23. All organisms were similar in the normal ultrastructure of individual cells and in the ability to form abnormal, pathological ehrlichial cells of the same type irrespective of the species. Normally all ehrlichiae studied in cell culture existed in two morphological forms – reticulate and dense-cored cells, both of which could divide by binary fission. Most alterations were related to their membranes, especially the cell wall. Differences in the structure of intravacuolar microcolonies (morulae) of ehrlichiae and their inter-relations with the host cells allowed differentiation of the genogroups: the genogroup formed large morulae, with many ehrlichiae, often suspended in a fibrillar matrix, and the host cell mitochondria and endoplasmic reticulum usually aggregated near the morulae and were in contact with the morula membrane; the -HGE group morulae had no fibrillar matrix, no contacts with host cell mitochodria, and they did not aggregate around the morulae; group usually developed in small individual vacuoles that did not fuse with each other and divided along with the ehrlichiae.

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1998-03-01
2022-10-03
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References

  1. Smith R. D., Sells D. M., Stephenson E. H., Ristic M. R., Huxsoll D. L. Development of Ehrlichia canis, causative agent of canine ehrlichiosis, in the tick Rhipicephalus sanguineus and its differentiation from a symbiotic rickettsia. Am J Vet Res 1976; 37:119–126
    [Google Scholar]
  2. Anderson B. E., Sims K. G., Olson J. G. Amblyomma americanum: a potential vector of human ehrlichiosis. Am J Trop Med Hyg 1993; 49:239–244
    [Google Scholar]
  3. Magnarelli L. A., Stafford K. C., Mather T. N., Yeh M.-T., Horn K. D., Dumler J. S. Hemocytic rickettsia-like organisms in ticks: serologic reactivity with antisera to ehrlichiae and detection of DNA of agent of human granulocytic ehrlichiosis by PCR. J Clin Microbiol 1995; 33:2710–2714
    [Google Scholar]
  4. Pancholi P., Kolbert C. P., Mitchell P. D. Ixodes dammini as a potential vector of human granulocytic ehrlichiosis. J Infect Dis 1995; 172:1007–1012
    [Google Scholar]
  5. Kelly D. J., Lee M., Lewis G. E. A light and electron microscopic examination of Ehrlichia sennetsu in cultured human endothelial cells. Jpn J Med Sci Biol 1985; 38:155–168
    [Google Scholar]
  6. Maeda K., Markowitz N., Hawley R. C., Ristic M., Cox D., McDade J. E. Human infection with Ehrlichia canis, a leukocytic rickettsia. N Engl J Med 1987; 316:853–856
    [Google Scholar]
  7. Anderson B. E., Dawson J. E., Jones D. C., Wilson K. H. Ehrlichia chaffeensis, a new species associated with human ehrlichiosis. J Clin Microbiol 1991; 29:2838–2842
    [Google Scholar]
  8. Chen S.-M., Dumler J. S., Bakken J. S., Walker D. H. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J Clin Microbiol 1994; 32:589–595
    [Google Scholar]
  9. Walker D. H., Dumler J. S. Emergence of the ehrlichioses as human health problems. Emerg Infect Dis 1996; 2:18–29
    [Google Scholar]
  10. Kawahara M., Suto C., Rikihisa Y., Yamamoto S., Tsuboi Y. Characterization of ehrlichial organisms isolated from a wild mouse. J Clin Microbiol 1993; 31:89–96
    [Google Scholar]
  11. Wen B., Rikihisa Y., Mott Y., Fuerst P. A., Kawahara M., Suto C. Ehrlichia muris sp. nov., identified on the basis of 16S rRNA base sequences and serological, morphological, and biological characteristics. Int J Syst Bacteriol 1995; 45:250–254
    [Google Scholar]
  12. Hart A., Kocan K. M., Bezuidenhout J. D., Prozesky L. Ultrastructural morphology of Cowdria ruminantium in midgut epithelial cells of adult Amblyomma hebraeum female ticks. Onderstepoort J Vet Res 1991; 58:187–193
    [Google Scholar]
  13. Jongejan F., Zandbergen T. A., van de Wiel P. A., de Groot M., Uilenberg G. The tick-borne rickettsia Cowdria ruminantium has a Chlamydia-like developmental cycle. Onderstepoort J Vet Res 1991; 58:227–237
    [Google Scholar]
  14. Kocan K. M., Bezuidenhout J. D. Morphology and development of Cowdria ruminantium in Amblyomma ticks. Onderstepoort J Vet Res 1987; 54:177–182
    [Google Scholar]
  15. Kocan K. M., Bezuidenhout J. D., Hart A. Ultrastructural features of Cowdria ruminantium in midgut epithelial cells and salivary glands of nymphal Amblyomma hebraeum. Onderstepoort J Vet Res 1987; 54:87–92
    [Google Scholar]
  16. Kocan K. M., Morzaria S. P., Voigt W. P., Kiarie J., Irvin A. D. Demonstration of colonies of Cowdria ruminantium in midgut epithelial cells of Amblyomma variegatum. Am J Vet Res 1987; 48:356–360
    [Google Scholar]
  17. Dumler J. S., Asanovich K. M., Bakken J. S., Richter P., Kimsey R., Madigan J. E. Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and human granulocytic ehrlichia. J Clin Microbiol 1995; 33:1098–1103
    [Google Scholar]
  18. Harvey J. W., Simpson C. F., Gaskin J. M. Cyclic thrombocytopenia induced by a Rickettsia-like agent in dogs. J Infect Dis 1978; 137:182–188
    [Google Scholar]
  19. Kocan K. M., Stich R. W., Claypool P. L., Ewing S. A., Hair J. A., Barron S. J. Intermediate site of development of Anaplasma marginale in feeding adult Dermacentor andersoni ticks that were infected as nymphs. Am J Vet Res 1990; 51:128–132
    [Google Scholar]
  20. Kocan K. M., Stiller D. Development of Anaplasma ovis (Rickettsiales Anaplasmataceae) in male Dermacentor andersoni (Acari Ixodidae) transferred from infected to susceptible sheep. J Med Entomol 1992; 29:98–107
    [Google Scholar]
  21. Kocan K. M., Stiller D., Goff W. L. Development of Anaplasma marginale in male Dermacentor andersoni transferred from parasitemic to susceptible cattle. Am J Vet Res 1992; 53:499–507
    [Google Scholar]
  22. Chaichanasiriwithaya W., Rikihisa Y., Yamamoto S. Antigenic, morphologic, and molecular characterization of new Ehrlichia risticii isolates. J Clin Microbiol 1994; 38:3026–3033
    [Google Scholar]
  23. Rikihisa Y. Cross-reacting antigens between Neorickettsia helminthoeca and Ehrlichia species, shown by immunofluorescence and western immunoblotting. J Clin Microbiol 1991; 29:2024–2029
    [Google Scholar]
  24. Rikihisa Y., Stills H., Zimmerman G. Isolation and continuous culture of Neorickettsia helminthoeca in a macrophage cell line. J Clin Microbiol 1991; 29:1928–1933
    [Google Scholar]
  25. Wen B., Rikihisa Y., Yamamoto S., Kawabata N., Fuerst P. A. Characterisation of the SF agent, an Ehrlichia sp. isolated from the fluke Stellantchasmus falcatus, by 16S rRNA base sequence, serological, and morphological analyses. Int J Syst Bacteriol 1996; 46:149–154
    [Google Scholar]
  26. Brouqui P., Birg M. L., Raoult D. Cytopathic effect, plaque formation, and lysis of Ehrlichia chaffeensis grown on continuous cell lines. Infect Immun 1994; 62:405–411
    [Google Scholar]
  27. Chen S.-M., Popov V. L., Feng H.-M., Wen J., Walker D. H. Cultivation of Ehrlichia chaffeensis in mouse embryo, Vero, BGM, and L929 cells and study of Ehrlichia-induced cytopathic effect and plaque formation. Infect Immun 1995; 63:647–655
    [Google Scholar]
  28. Goodman J. L., Nelson C., Vitale B. Direct cultivation of the causative agent of human granulocytic ehrlichiosis. N Engl J Med 1996; 334:209–215
    [Google Scholar]
  29. Munderloh U. G., Madigan J. E., Dumler J. S. Isolation of the equine granulocytic ehrlichiosis agent, Ehrlichia equi, in tick cell culture. J Clin Microbiol 1996; 34:664–670
    [Google Scholar]
  30. Rikihisa Y., Perry B. D. Causative ehrlichial organisms in Potomac horse fever. Infect Immun 1985; 49:513–517
    [Google Scholar]
  31. Wells M. Y., Rikihisa Y. Lack of lysosomal fusion with phagosomes containing Ehrlichia risticii in P388D1 cells: abrogation of inhibition with oxytetracycline. Infect Immun 1988; 56:3209–3215
    [Google Scholar]
  32. Rikihisa Y. The tribe Ehrlichieae and ehrlichial diseases. Clin Microbiol Rev 1991; 4:286–308
    [Google Scholar]
  33. Dawson J. E., Rikihisa Y., Ewing S. A., Fishbein D. B. Serologic diagnosis of human ehrlichiosis using two Ehrlichia canis isolates. J Infect Dis 1991; 163:564–567
    [Google Scholar]
  34. Dawson J. E., Anderson B. E., Fishbein D. B. Isolation and characterization of an Ehrlichia sp. from a patient diagnosed with human ehrlichiosis. J Clin Microbiol 1991; 29:2741–2745
    [Google Scholar]
  35. Messick J. B., Rikihisa Y. Presence of parasite antigen of the surface of P388D] cells infected with Ehrlichia risticii. Infect Immun 1992; 60:3079–3086
    [Google Scholar]
  36. Iqbal Z., Rikihisa Y. Reisolation of Ehrlichia canis from blood and tissues of dogs after doxycycline treatment. J Clin Microbiol 1994; 32:1644–1649
    [Google Scholar]
  37. Popov V. L., Chen S.-M., Feng H.-M., Walker D. H. Ultrastructural variation of cultured Ehrlichia chaffeensis. J Med Microbiol 1995; 43:411–421
    [Google Scholar]
  38. Dumler J. S., Chen S.-M., Asanovich K., Trigiani E., Popov V. L., Walker D. H. Isolation of characterization of a new strain of Ehrlichia chaffeensis from a patient with nearly fatal monocytic ehrlichiosis. J Clin Microbiol 1995; 33:1704–1711
    [Google Scholar]
  39. Rikihisa Y., Zhi N., Wormser G. P., Wen B., Horowitz H. W., Hechemy K. E. Ultrastructural and antigenic characterization of a granulocytic ehrlichiosis agent directly isolated and stably cultivated from a patient in New York state. J Infect Dis 1997; 175:210–213
    [Google Scholar]
  40. Chen S.-M., Yu X.-J., Popov V. L., Westerman E. L., Hamilton F. G., Walker D. H. Genetic and antigenic diversity of Ehrlichia chaffeensis: comparative analysis of a novel human strain from Oklahoma and previously isolated strains. J Infect Dis 1997; 175:856–863
    [Google Scholar]
  41. O’Neill S. L., Pettigrew M. M., Sinkins S. P., Braig H. R., Andreadis T. G., Tesh R. B. In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. Insect Mol Biol 1997; 6:33–39
    [Google Scholar]
  42. Madigan J. E., Richter P. J., Kimsey R. B., Barlough J. E., Bakken J. S., Dumler J. S. Transmission and passage in horses of the agent of human granulocytic ehrlichiosis. J Infect Dis 1995; 172:1141–1144
    [Google Scholar]
  43. Ito S., Rikihisa Y. Techniques for electron microscopy of rickettsiae. In Burgdorfer W., Anacker R. L. (eds) Rickettsiae and rickettsial diseases New York: Academic Press; 1981213–227
    [Google Scholar]
  44. Perez M., Rikihisa Y., Wen B. Ehrlichia canis-like agent isolated from a man in Venezuela: antigenic and genetic characterization. J Clin Microbiol 1996; 34:2133–2139
    [Google Scholar]
  45. Rikihisa Y., Logan L. L. Unusual peptidoglycan-like substance in Cowdria ruminantium in the endothelial cells of the choroid plexus of the goat. Proceedings of the XIth International Congress on Electron Microscopy 19863345–3346
    [Google Scholar]
  46. Rikihisa Y., Perry B. D., Cordes D. O. Ultrastructural study of ehrlichial organisms in the large colons of ponies infected with Potomac horse fever. Infect Immun 1985; 49:505–512
    [Google Scholar]
  47. Kocan K. M., Hair J. A., Ewing S. A. Ultrastructure of Anaplasma marginale Theiler in Dermacentor andersoni Stiles and Dermacentor variabilis (Say). Am J Vet Res 1980; 41:1966–1976
    [Google Scholar]
  48. Kocan K. M., Ewing S. A., Holbert D., Hair J. A. Morphologic characteristics of colonies of Anaplasma marginale Theiler in midgut epithelial cells of Dermacentor andersoni Stiles. Am J Vet Res 1982; 43:586–593
    [Google Scholar]
  49. Potgieter F. T., Kocan K. M., McNew R. W., Ewing S. A. Demonstration of colonies of Anaplasma marginale in the midgut of Rhipicephalus simus. Am J Vet Res 1983; 44:2256–2261
    [Google Scholar]
  50. Kocan K. M., Yellin T. N., Ewing S. A., Hair J. A., Barron S. J. Morphology of colonies of Anaplasma marginale in nymphal Dermacentor andersoni. Am J Vet Res 1984; 45:1434–1440
    [Google Scholar]
  51. Avakyan A. A., Popov V. L. Rickettsiaceae and Chlamydiaceae: comparative electron microscopic studies. Acta Virol 1984; 28:159–173
    [Google Scholar]
  52. Domingue G. J., Woody H. B. Bacterial persistence and expression of disease. Clin Microbiol Rev 1997; 10:320–344
    [Google Scholar]
  53. Rikihisa Y., Zhang Y., Park J. Inhibition of infection of macrophages with Ehrlichia risticii by cytochalasins, mono-dansylcadaverine, and taxol. Infect Immun 1994; 62:5126–5132
    [Google Scholar]
  54. Hildebrandt P. K., Conroy J. D., McKee A. E., Nyindo M. B. A., Huxsoll D. L. Ultrastructure of Ehrlichia canis. Infect Immun 1973; 7:265–271
    [Google Scholar]
  55. Tuomi J., von Bonsdorff C.-H. Electron microscopy of tick-borne fever agent in bovine and ovine phagocytizing leukocytes. J Bacteriol 1996; 92:1478–1492
    [Google Scholar]
  56. Sells D. M., Hildebrandt P. K., Lewis G. E., Nyindo M. B. A., Ristic M. Ultrastructural observations on Ehrlichia equi organisms in equine granulocytes. Infect Immun 1976; 13:273–280
    [Google Scholar]
  57. Taraska T., Ward D. M., Ajioka R. S. The late chlamydial inclusion membrane is not derived from the endocytic pathway and is relatively deficient in host proteins. Infect Immun 1996; 64:3713–3727
    [Google Scholar]
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