%0 Journal Article %A GrØndahl, Marie Louise %A Jensen, Gerda M. %A Nielsen, C. G. %A Skadhauge, E. %A Olsen, J. E. %A Hansen, M. B. %T Secretory pathways in Salmonella Typhimurium-induced fluid accumulation in the porcine small intestine %D 1998 %J Journal of Medical Microbiology, %V 47 %N 2 %P 151-157 %@ 1473-5644 %R https://doi.org/10.1099/00222615-47-2-151 %I Microbiology Society, %X The involvement of 5-hydroxytryptamine (5-HT) and 5-HT3 receptors and prostaglandin E2 (PGE2) in Salmonella Typhimurium-induced fluid accumulation in the porcine small intestine was investigated. Salmonella Typhimurium (108 and 1010 cfu) and cholera toxin (CT; 20 μg) were instilled for 8 and 11 h in ligated loops in the porcine jejunum and ileum. Fluid accumulation and concentrations of Na+, K+, Cl−, 5-HT and PGE2 in the fluid accumulated in the loops were measured. The fluid accumulation was also measured when Salmonella Typhimurium (1010 cfu) and CT (20 μg) were instilled for 8 h in ligated loops in jejunum and ileum in pigs given subcutaneous injections of saline or the 5-HT3 receptor antagonist ondansetron (200 μg/kg). Salmonella Typhimurium (1010 cfu) and CT both induced fluid accumulation in jejunum and ileum after 8 and 11 h. Both treatments also induced an increase in luminal release of 5-HT and PGE2. The accumulated fluid was iso-osmotic and hyperosmotic in CT- and Salmonella Typhimurium-treated loops, respectively. Ondansetron reduced the Typhimurium-induced fluid accumulation in both jejunum and ileum by c. 40%, while it failed to reduce the response to CT. These results demonstrate that 5-HT and PGE2 are released and 5-HT3 receptors activated in the secretory pathway of Typhimurium in the porcine small intestine. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-47-2-151