The involvement of 5-hydroxytryptamine (5-HT) and 5-HT receptors and prostaglandin E (PGE) in Typhimurium-induced fluid accumulation in the porcine small intestine was investigated. Typhimurium (10 and 10 cfu) and cholera toxin (CT; 20 μg) were instilled for 8 and 11 h in ligated loops in the porcine jejunum and ileum. Fluid accumulation and concentrations of Na, K, Cl, 5-HT and PGE in the fluid accumulated in the loops were measured. The fluid accumulation was also measured when Typhimurium (10 cfu) and CT (20 μg) were instilled for 8 h in ligated loops in jejunum and ileum in pigs given subcutaneous injections of saline or the 5-HT receptor antagonist ondansetron (200 μg/kg). Typhimurium (10 cfu) and CT both induced fluid accumulation in jejunum and ileum after 8 and 11 h. Both treatments also induced an increase in luminal release of 5-HT and PGE. The accumulated fluid was iso-osmotic and hyperosmotic in CT- and Typhimurium-treated loops, respectively. Ondansetron reduced the Typhimurium-induced fluid accumulation in both jejunum and ileum by 40%, while it failed to reduce the response to CT. These results demonstrate that 5-HT and PGE are released and 5-HT receptors activated in the secretory pathway of Typhimurium in the porcine small intestine.


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