1887

Journal of Medical Microbiology logo

Volume 47, Issue 2

Interactions between species and platelets Free

Abstract

spp. are able to cause disseminated disease in immunocompromised patients. This study examined the interactions of spp. with platelets, complement and polymorphonuclear leucocytes (PMNLs). With the exception of , all other spp., including a strain previously classified as , aggregated human platelets at a ratio of yeast cells: platelets of 1:80. Usually, those species and strains that aggregated platelets were either killed or prevented from growing in platelet-rich plasma indicating that the aggregation released microbicidal or microbistatic substances that were active against spp. All spp. were resistant to attack by complement in 50% serum. However, all species activated complement as determined by the presence of C3 fragments on their surface, in particular a 195-kDa fragment corresponding to C3c, two fragments at 67 and 40 kDa corresponding to iC3b, and a 33-kDa fragment corresponding to C3d. When strains were tested for their ability to stimulate the release of pro-inflammatory substances from platelets and PMNLs, it was found that most strains stimulated PMNLs to release interleukin(IL)-8 but not IL-1 or leucotriene B. The ability of to evade complement-mediated killing and not to aggregate platelets may contribute to the survival of this species in the blood during vascular infections.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© 1998 The Pathological Society of Great Britain and Ireland
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-47-2-103
1998-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/47/2/medmicro-47-2-103.html?itemId=/content/journal/jmm/10.1099/00222615-47-2-103&mimeType=html&fmt=ahah

References

  1. Odds F. C. Candida and candidosis. 2nd edn London: Bailliere Tindall; 1988175–180
     [Google Scholar]
  2. MacFarlane T. W., Samaranayake L. P. Fungal infections. In Clinical oral microbiology London: Wright; 1989122–139
     [Google Scholar]
  3. Scheld W. M., Sande M. A. Endocarditis and intravascular infections. In Mandell G. L., Bennett J. E., Dobin R. (eds) Principles and practice of infectious diseases vol 1 4th edn New York: Churchill Livingstone; 1995740–783
     [Google Scholar]
  4. Walsh T. J., Pizzo P. A. Treatment of systemic fungal infections: recent progress and current problems. Eur J Clin Microbiol Infect Dis 1988; 7:460–475
     [Google Scholar]
  5. Reyes M. P., Lemer A. M. Endocarditis caused by Candida species. In Body G. P., Fainstein V. (eds) Candidiasis New York: Raven Press; 1985203–209
     [Google Scholar]
  6. Hallum J. L., Williams T. W. Candida endocarditis. In Bodey G. P. (ed) Candidiasis: pathogenesis, diagnosis and treatment New York: Raven Press; 1993257–369
     [Google Scholar]
  7. Jakab E., Paulsson M., Ascencio F., Ljungh A. Expression of vitronectin and fibronectin binding by Candida albicans yeast cells. APMIS 1993; 101:187–193
     [Google Scholar]
  8. Filler S. G., Pfunder A. S., Spellberg B. J., Spellberg J. P., Edwards J. E. Candida albicans stimulates cytokine production and leukocyte adhesion molecule expression by endothelial cells. Infect Immun 1996; 64:2609–2617
     [Google Scholar]
  9. Maisch P. A., Calderone R. A. Role of surface mannan in adherence of Candida albicans to fibrin-platelet clots formed in vitro. Infect Immun 1981; 32:92–97
     [Google Scholar]
  10. Ford I., Douglas C. W. I., Heath J., Rees C., Preston F. E. Evidence for the involvement of complement proteins in platelet aggregation by Streptococcus sanguis NCTC 7863. Br J Haematol 1996; 94:729–739
     [Google Scholar]
  11. Lehrer R. I., Barton A., Daher K. A., Harwig S. S. L., Ganz T., Selsted M. E. Interaction of human defensins with Escherichia coli. Mechanisms of bactericidal activity. J Clin Invest 1989; 84:553–561
     [Google Scholar]
  12. Spitznagel J. K. Antibiotic proteins of human neutrophils. J Clin Invest 1990; 86:1381–1386
     [Google Scholar]
  13. Yeaman M. R., Tang Y.-Q., Shen A. J., Bayer A. S. Selsted ME. Purification and in vitro activities of rabbit platelet microbicidal proteins. Infect Immun 1997; 65:1023–1031
     [Google Scholar]
  14. Domer J. E., Lehrer R. I. Introduction to Candida: systemic candidiasis. In Murphy J. W., Freidman H., Bendinelli M. (eds) Fungal infections and immune responses New York: Plenum Press; 199349–116
     [Google Scholar]
  15. Miles A. A., Misra S. S., Irwin J. O. The estimation of the bactericidal power of the blood. J Hyg 1938; 38:732–748
     [Google Scholar]
  16. Gordon D. L., Rice J., Finlay-Jones J. J., McDonald P. J., Hostetter M. K. Analysis of C3 deposition and degradation on bacterial surfaces after opsonization. J Infect Dis 1988; 157:697–704
     [Google Scholar]
  17. Klotz S. A., Harrison J. L., Misra R. P. Aggregated platelets enhance adherence of Candida yeasts to endothelium. J Infect Dis 1989; 160:669–677
     [Google Scholar]
  18. Skerl K. G., Calderone R. A., Sreevalson T. Platelet interactions with Candida albicans. Infect Immun 1981; 34:938–943
     [Google Scholar]
  19. Cameron B. J., Douglas L. J. Blood group glycolipids as epithelial cells receptors for Candida albicans. Infect Immun 1996; 64:891–896
     [Google Scholar]
  20. Gartner T. K., Bennett J. S. The tetrapeptide analogue of the cell attachment site of fibronectin inhibits platelet aggregation and fibrinogen binding to activated platelets. J Biol Chem 1985; 260:11891–11894
     [Google Scholar]
  21. Mohri H., Ohkubo T. How vitronectin binds to activated glycoprotein IIb-IIIa complex and its function in platelet aggregation. Am J Clin Pathol 1991; 96:605–609
     [Google Scholar]
  22. Preissner K. T., Jenne D. Structure of vitronectin and its biological role in homeostasis. Thromb Haemost 1991; 66:123–132
     [Google Scholar]
  23. DeMuri G. P., Hostetter M. K. Evidence for a β 1 integrin fibronectin receptor in Candida tropicalis. J Infect Dis 1996; 174:127–132
     [Google Scholar]
  24. Herzberg M. C., MacFarlane G. D., Gong K. The platelet interactivity phenotype of Streptococcus sanguis influences the course of experimental endocarditis. Infect Immun 1992; 60:4809–4818
     [Google Scholar]
  25. Koo S.-P., Yeaman M. R., Bayer A. S. Staphylocidal action of thrombin-induced platelet microbicidal protein is influenced by microenvironment and target cell growth phase. Infect Immun 1996; 64:3758–3764
     [Google Scholar]
  26. Oppenheim J. J., Zachariae C. O. C., Mukaida N., Matsushima K. Properties of the novel proinflammatory supergene “intercrine” cytokine family. Annu Rev Immunol 1991; 9:617–648
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-47-2-103
Loading
Interactions between Candida species and platelets
J. Med. Microbiol. 47, 103 (1998); https://doi.org/10.1099/00222615-47-2-103
/content/journal/jmm/10.1099/00222615-47-2-103
/content/journal/jmm/10.1099/00222615-47-2-103
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error