1887

Abstract

Summary

The ability of the RC14 biosurfactant ‘surlactin’ to inhibit the initial adhesion of various uropathogenic bacteria and two yeast strains to silicone rubber was investigated in a parallel-plate flow chamber in filter-sterilised pooled human urine. A parallel-plate flow chamber with a silicone rubber bottom plate was filled with a 1.0 mg/ml biosurfactant solution for adsorption overnight (18 h). Subsequently, the adhesion of the bacterial or yeast cells from a urine suspension under low flow (shear rate 15 s) was followed by automated image analysis. Control tests were with untreated silicone rubber. Initial deposition rates and numbers of adhering cells after 4 h of flow were determined. Surlactin layers caused a marked inhibition of the initial deposition rates and adhesion numbers after 4 h for the majority of the bacteria (11 of 15 strains tested) and this inhibition was particularly effective against and . Although the initial deposition rates of the two strains were reduced by . 50% in comparison with the controls, the numbers of yeast cells adhering after 4 h were similar.

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1998-12-01
2022-06-27
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References

  1. Nickel J. C., Feero P., Costerton J. W., Wilson E. Incidence and importance of bacteriuria in postoperative, short-term urinary catheterization. Can J Surg 1989; 32:131–132
    [Google Scholar]
  2. Kunin C. M., McCormack R. C. Prevention of catheter-induced urinary-tract infections by sterile closed drainage. N Engl J Med 1966; 274:1155–1161
    [Google Scholar]
  3. Warren J. W., Platt R., Thomas R. J., Rosner B., Kass E. H. Antibiotic irrigation and catheter-associated urinary-tract infections. N Engl J Med 1978; 299:570–573
    [Google Scholar]
  4. Garibaldi R. A., Burke J. P., Dickman M. L., Smith C. B. Factors predisposing to bacteriuria during indwelling urethral catheterization. N Engl J Med 1974; 291:215–219
    [Google Scholar]
  5. Warren J. W., Muncie H. L., Bergquist E. J., Hoopes J. M. Sequelae and management of urinary infection in the patient requiring chronic catheterization. J Urol 1981; 125:1–8
    [Google Scholar]
  6. Platt R., Polk B. F., Murdock B., Rosner B. Risk factors for nosocomial urinary tract infection. Am J Epidemiol 1986; 124:977–985
    [Google Scholar]
  7. Neu H. C. Urinary tract infections. Am J Med 1992; 92: Suppl 4A63A–70S
    [Google Scholar]
  8. Warren J. W., Damron D., Tenney J. H., Hoopes J. M., Deforge B., Muncie H. L. Fever, bacteremia, and death as complications of bacteriuria in women with long-term urethral catheters. J Infect Dis 1987; 155:1151–1158
    [Google Scholar]
  9. Warren J. W., Tenney J. H., Hoopes J. M., Muncie H. L., Anthony W. C. A prospective microbiologic study of bacteriuria in patients with chronic indwelling urethral catheters. J Infect Dis 1982; 146:719–723
    [Google Scholar]
  10. Stickler D. J. Bacterial biofilms and the encrustation of urethral catheters. Biofouling 1996; 9:293–305
    [Google Scholar]
  11. Wise G. J., Silver D. A. Fungal infections of the genitourinary system. J Urol 1993; 149:1377–1388
    [Google Scholar]
  12. Nickel J. C., Downey J. A., Costerton J. W. Ultrastructural study of microbiologic colonization of urinary catheters. Urology 1989; 34:284–291
    [Google Scholar]
  13. Molander U., Milsom I., Ekelund P., Mellstrӧm D., Eriksson O. Effect of oral oestriol on vaginal flora and cytology and urogenital symptoms in the post-menopause. Maturitas 1990; 12:113–120
    [Google Scholar]
  14. Raz R., Stamm W. E. A controlled trial of intravaginal estriol in postmanopausal women with recurrent urinary tract infections. N Engl J Med 1993; 329:753–756
    [Google Scholar]
  15. Reid G., Bruce A. W., Taylor M. Influence of three-day antimicrobial therapy and Lactobacillus vaginal suppositories on recurrence of urinary tract infections. Clin Ther 1992; 14:11–16
    [Google Scholar]
  16. Reid G., Bruce A. W., Taylor M. Instillation of Lactobacillus and stimulation of indigenous organisms to prevent recurrence of urinary tract infections. Microecol Ther 1995; 23:32–45
    [Google Scholar]
  17. Reid G., Tieszer C. Preferential adhesion of urethral bacteria from a mixed population to a urinary catheter. Cells Mater 1993; 3:171–173
    [Google Scholar]
  18. Millsap K., Reid G., van der Mei H. C., Busscher H. J. Displacement of Enterococcus faecalis from hydrophobic and hydrophilic substrata by Lactobacillus and Streptococcus spp. as studied in a parallel plate flow chamber. Appl Environ Microbiol 1994; 60:1867–1874
    [Google Scholar]
  19. Chan R. C. Y., Reid G., Irvin R. T., Bruce A. W., Costerton J. W. Competitive exclusion of uropathogens from human uroepithelial cells by Lactobacillus whole cells and cell wall fragments. Infect Immun 1985; 47:84–89
    [Google Scholar]
  20. Hawthorn L. A., Reid G. Exclusion of uropathogen adhesion to polymer surfaces by Lactobacillus acidophilus. J Biomed Mater Res 1990; 24:39–46
    [Google Scholar]
  21. Klaenhammer T. R. Bacteriocins of lactic acid bacteria. Biochimie 1988; 70:337–349
    [Google Scholar]
  22. Velraeds M. M. C., van der Mei H. C., Reid G., Busscher H. J. Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates. Appl Environ Microbiol 1996; 62:1958–1963
    [Google Scholar]
  23. Velraeds M. M. C., van der Mei H. C., Reid G., Busscher H. J. Inhibition of initial adhesion of uropathogenic Enterococcus faecalis to solid substrata by an adsorbed biosurfactant layer from Lactobacillus acidophilus. Urology 1997; 49:790–794
    [Google Scholar]
  24. Velraeds M. M. C., van der Mei H. C., Reid G., Busscher H. J. Physico-chemical and biochemical characterization of biosurfactants released by Lactobacillus strains. Colloids Surf B: Biointerfaces 1996; 8:51–61
    [Google Scholar]
  25. Reid G., Cuperus P. L., Bruce A. W. Comparison of contact angles and adhesion to hexadecane of urogenital, dairy, and poultry lactobacilli: effect of serial culture passages. Appl Environ Microbiol 1992; 58:1549–1553
    [Google Scholar]
  26. De Man J. C., Rogosa M., Sharpe M. E. A medium for cultivation of lactobacilli. J Appl Microbiol 1969; 23:130–135
    [Google Scholar]
  27. Reid G., Navarro R., Velraeds M., van der Mei H. C., Busscher H. J. Inhibitory activity of different Lactobacillus biosurfactant fractions against enterococcal retention to surfaces. Infect Immun 1997 submitted
    [Google Scholar]
  28. Neu T. R. Significance of bacterial surface-active compounds in interaction of bacteria with interfaces. Microbiol Rev 1996; 60:151–166
    [Google Scholar]
  29. Jenny K., Deltrieu V., Kӓppeli O. Lipopeptide production by Bacillus Licheniformis. In Kosaric N. (ed) Biosurfactants-production, properties, applications (Surfactant Science Series, vol. 48.) New York: M Dekker; 1993135–156
    [Google Scholar]
  30. Neu T. R., Hӓrtner T., Poralla K. Surface active properties of viscosin: a peptidolipid antibiotic. Appl Microbiol Biotechnol 1990; 32:518–520
    [Google Scholar]
  31. Pratt-Terpstra I. H., Weerkamp A. H., Busscher H. J. Microbial factors in a thermodynamic approach of oral streptococcal adhesion to solid substrata. J Colloid Interface Sci 1988; 129:568–574
    [Google Scholar]
  32. Busscher H. J., Bellon-Fontaine M.-N. Mozes, Deposition of Leuconostoc mesenteroides and Streptococcus thermophilus to solid substrata in a parallel plate flow cell. Bio fouling 1990; 2:55–63
    [Google Scholar]
  33. Mobley H. L. T., Warren J. W. Urease-positive bacteriuria and obstruction of long-term urinary catheters. J Clin Microbiol 1987; 25:2216–2217
    [Google Scholar]
  34. Ørskov I., Ferencz A., Ørskov A. Tamm-Horsfall protein or uromucoid is the normal urinary slime that traps type 1 fimbriated Escherichia coli. Lancet 1980; 1:887
    [Google Scholar]
  35. Hawthorn L., Reid G. The effect of protein and urine on uropathogen adhesion to polymer substrata. J Biomed Mater Res 1990; 24:1325–1332
    [Google Scholar]
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