1887

Abstract

Summary

The relative pathogenicity of and was investigated by assessing their colonisation and infectivity of the Sprague-Dawley rat oral mucosa. During an initial 21-week period with intermittent oral inoculation, both spp. demonstrated variable surface colonisation of the oral mucosa. After 3 days of oral inoculation, both yeast species were recovered from all animals. During the 21-week period the mean oral load of in the control group of rats varied between (26–274) × 10 cfu/ml whereas the two test groups of rats carrying CK9 and CK13 had a mean load of (2–10) × 10 cfu/ml. Although oral colonisation by was greater than that of , neither species induced candidal infection during this period. Subsequent immunosuppression of the rats by intramuscular cyclophosphamide (40 mg/kg body weight) initiated infection of the dorsal tongue (around the conical papillae area) after 4 weeks, in all of three animals while similar lesions due to were seen – albeit after 5–7 weeks – in three of eight animals. Characteristic histological changes of mucosal candidosis were discernible on the lingual mucosa of rats infected with both spp. including parakeratosis, absence of a stratum granulosum, thickened rete ridges, micro-abscess formation and polymorph infiltration of the lingual epithelium. Although both species produced fungal hyphae that penetrated the epithelium up to the prickle cell layer, hyphae tended to be relatively more profuse. Taken together these results substantiate, for the first time in an animal model, the clinical evidence that , once considered an innocuous commensal, is capable of transforming into an invasive pathogen under conditions of immunosuppression.

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1998-12-01
2022-09-28
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References

  1. Cawson R. A., Lehner T. Chronic hyperplastic candidiasis-candidal leukoplakia. Br J Dermatol 1968; 80:9–16
    [Google Scholar]
  2. Cawson R. A. Chronic oral candidosis, denture stomatitis and chronic hyperplastic candidosis. In Winner H. I., Hurley R. (eds) Symposium on Candida infections Edinburgh: Livingstone; 1966138–153
    [Google Scholar]
  3. Howlett J. A. The infection of rat tongue mucosa in vitro with five species of Candida. J Med Microbiol 1976; 9:309–316
    [Google Scholar]
  4. Partridge B. M. The use of the chorioallantoic membrane of the developing chick for culture of dermatophytes: a modified technic; a preliminary report upon its use for serial passage. J Invest Dermatol 1959; 32:605–619
    [Google Scholar]
  5. Jones J. H., Adams D. Experimentally induced acute oral candidosis in the rat. Br J Dermatol 1970; 83:670–673
    [Google Scholar]
  6. Adams D., Jones J. H. Life history of experimentally induced acute oral candidiasis in the rat. J Dent Res 1971; 50:643–644
    [Google Scholar]
  7. Jones J. H., Russell C. The histology of chronic candidal infection of the rat’s tongue and its relevance to human oral leukoplakia. J Clin Pathol 1973; 26:390
    [Google Scholar]
  8. Jones J. H., Russell C. The time scale of penetration of the rat’s oral epithelium by C. albicans. International Research Communications System 197373–126–3–5
    [Google Scholar]
  9. Allen C. M., Beck F. M. Strain-related differences in pathogenicity of Candida albicans for oral mucosa. J Infect Dis 1983; 147:1036–1040
    [Google Scholar]
  10. Allen C. M., Beck F. M., Lurie F. A., Pinsky H. M. Role of tetracycline in pathogenesis of chronic candidiasis of rat tongues. Infect Immun 1985; 47:480–483
    [Google Scholar]
  11. Allen C. M., Beck F. M. Differences in mucosal reaction related to Candida albicans isolates. J Oral Pathol 1987; 16:89–93
    [Google Scholar]
  12. Allen C. M. Animal models of oral candidiasis: a review. Oral Surg Oral Med Oral Pathol 1994; 78:216–221
    [Google Scholar]
  13. Horn R., Wong B., Kiehn T. E., Armstrong D. Fungemia in a cancer hospital: changing frequency, earlier onset, and results of therapy. Rev Infect Dis 1985; 7:646–655
    [Google Scholar]
  14. Wingard J. R., Merz W. G., Rinaldi M. G., Johnson T. R., Karp J. E., Saral R. Increase in Candida krusei infection among patients with bone marrow transplantation and neutropenia treated prophylactically with fluconazole. N Engl J Med 1991; 325:1274–1277
    [Google Scholar]
  15. Samaranayake Y. H., Samaranayake L. P. Candida krusei: biology, epidemiology, pathogenicity and clinical manifestations of an emerging pathogen. J Med Microbiol 1994; 41:295–310
    [Google Scholar]
  16. Samaranayake Y. H., Wu P. C., Samaranayake L. P., So M., Yuen K. Y. Adhesion and colonisation of Candida krusei on host surfaces. J Med Microbiol 1994; 41:250–258
    [Google Scholar]
  17. Samaranayake Y. H., Wu P. C., Samaranayake L. P., So M. Relationship between the cell surface hydrophobicity and adherence of Candida krusei and Candida albicans to epithelial and denture acrylic surfaces. APMIS 1995; 103:707–713
    [Google Scholar]
  18. Silverman S., Migliorati C. A., Epstein J. B., Samaranayake L. P. Laboratory diagnosis of oral candidosis. In Samaranayake L. P., MacFarlane T. W. (eds) Oral candidosis London: Wright; 1990213–237
    [Google Scholar]
  19. Russell C., Jones J. H. Effects of oral inoculation of Candida albicans in tetracycline-treated rats. J Med Microbiol 1973; 6:275–279
    [Google Scholar]
  20. Darcie J. V., Lewis S. M. Basic haematological techniques, In: Practical haematology. 7th edn London: Churchill Livingstone; 1991
    [Google Scholar]
  21. Odds F. C. Candida and candidosis. 2nd edn London: Bailliere Tindall; 1988
    [Google Scholar]
  22. Budtz-Jorgensen E. Denture stomatitis IV An experimental model in monkeys. Acta Odontol Scand 1971; 29:513–526
    [Google Scholar]
  23. Budtz-Jorgensen E. Cellular immunity in acquired candidiasis of the palate. Scand J Dent Res 1973; 81:372–382
    [Google Scholar]
  24. Olsen I., Haanaes H. R. Experimental palatal candidosis and saliva-flow in monkeys. Scand J Dent Res 1977; 85:135–141
    [Google Scholar]
  25. Mackinnon J. E. Nuevo sentido de variacion en Mycotorula (monilia) albicans. Archivos Sociedad de Biologia de Montevideo 1936; 7:162–163
    [Google Scholar]
  26. Russell C., Jones J. H. The effects of oral inoculation of the yeast and mycelial phases of Candida albicans in rats fed on normal and carbohydrate rich diets. Arch Oral Biol 1973; 18:409–412
    [Google Scholar]
  27. Russell C., Jones J. H., Gibbs A. C. C. The carriage of Candida albicans in the mouths of rats treated with tetracycline briefly or for a prolonged period. Mycopathologia 1976; 58:125–129
    [Google Scholar]
  28. Allen C. M., Blozis G. G., Rosen S., Bright J. S. Chronic candidiasis of the rat tongue: a possible model for human median rhomboid glossitis. J Dent Res 1982; 61:1287–1291
    [Google Scholar]
  29. Fisker A. V., Rindom Schiott C., Philipsen H. P. Long-term oral candidosis in rats. APMIS 1982; 90:221–227
    [Google Scholar]
  30. Jones J. H., Russell C., Young C., Owen D. Tetracycline and the colonization and infection of the mouths of germ-free and conventionalized rats with Candida albicans. J Antimicrob Chemother 1976; 2:247–253
    [Google Scholar]
  31. Russell J. H., Jones J. H. The histology of prolonged candidal infection of the rat’s tongue. J Oral Pathol 1975; 4:330–339
    [Google Scholar]
  32. Hassan O. E., Jones J. H., Russell C. Experimental oral candidal infection and carriage of oral bacteria in rats subjected to a carbohydrate-rich diet and tetracycline treatment. J Med Microbiol 1985; 20:291–298
    [Google Scholar]
  33. Fisker A. Y., Rindom Schiott C., Philipsen H. P. Short-term oral candidosis in rats, with special reference to the site of infection. APMIS 1982; 90:49–57
    [Google Scholar]
  34. Samaranayake L. P., MacFarlane T. W. On the role of dietary carbohydrates in the pathogenesis of oral candidosis. FEMS Microbiol Lett 1985; 27:1–5
    [Google Scholar]
  35. Samaranayake L. P., Hughes A., Weetman D. A., MacFarlane T. W. Growth and acid production of Candida species in human saliva Supplemented with glucose. J Oral Pathol 1986; 15:251–254
    [Google Scholar]
  36. Samaranayake L. P., Hughes A., MacFarlane T. W. The proteolytic potential of Candida albicans in human saliva Supplemented with glucose. J Med Microbiol 1984; 17:13–22
    [Google Scholar]
  37. Samaranayake L. P., Raeside J., MacFarlane T. W. Factors affecting the phospholipase activity of Candida species in vitro. Sabouraudia 1984; 22:201–207
    [Google Scholar]
  38. Seelig M. S. Mechanisms by which antibiotics increase the incidence and severity of candidiasis and alter the immunological defenses. Bacteriol Rev 1966; 30:442–459
    [Google Scholar]
  39. Solomon P. Oral moniliasis complicating combined broad-spectrum antibiotic and antifungal therapy. N Engl J Med 1961; 265:847–848
    [Google Scholar]
  40. King R. D., Lee J. C., Morris A. L. Adherence of Candida albicans and other Candida species to mucosal epithelial cells. Infect Immun 1980; 27:667–674
    [Google Scholar]
  41. Tobgi R. S. Differences in the pathogenic potential of Candida species especially C. albicans. PhD thesis University of Glasgow; 1989
    [Google Scholar]
  42. Ferguson M. M. Oral mucous membrane markers of internal disease; part II: disorders of the endocrine system, haemopoietic system and nutrition. In Dolby A. D. (ed) Oral mucosa in health and disease Oxford: Blackwell; 1975249–250
    [Google Scholar]
  43. Merz W. G., Karp J. E., Schron D., Saral R. Increased incidence of fungemia caused by Candida krusei. J Clin Microbiol 1986; 24:581–584
    [Google Scholar]
  44. Nguyen V. Q., Penn R. L. Candida krusei infectious arthritis. A rare complication of neutropenia. Am J Med 1987; 83:963–965
    [Google Scholar]
  45. McQuillen D. P., Zingman B. S., Meunier F., Levitz S. M. Invasive infections due to Candida krusei: report of ten cases of fungemia that include three cases of endophthalmitis. Clin Infect Dis 1992; 14:472–478
    [Google Scholar]
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