RT Journal Article SR Electronic(1) A1 Appuhamy, S. A1 Low, J. C. A1 Coote, J. G. A1 Parton, R.YR 1998 T1 PCR methods and plasmid profile analysis for characterisation of Histophilus ovis strains JF Journal of Medical Microbiology, VO 47 IS 11 SP 987 OP 992 DO https://doi.org/10.1099/00222615-47-11-987 PB Microbiology Society, SN 1473-5644, AB Summary The value of polymerase chain reaction (PCR)-based DNA fingerprinting and plasmid profile analysis for differentiation of Histophilus ovis isolates was assessed. Nineteen isolates of H. ovis were typed by PCR-ribotyping, repetitive extragenic palindromic element (REP)-PCR and enterobacterial repetitive intergenic consensus (ERIC)-PCR. These methods distinguished five types by PCR-ribotyping, 11 types by REP-PCR and seven types by ERIC-PCR. The ribotyping method produced a relatively simple pattern and a small number of distinct types and was useful for differentiation of H. ovis from the phenotypically similar organism, Haemophilus somnus. REP- and ERIC-PCR both produced complex banding patterns, but increased the discrimination between strains. Plasmids were found in 12 of the 19 isolates and there were four different plasmid profiles. A combination of the PCR methods and plasmid profile analysis provided a high resolution typing method for H. ovis. , UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-47-11-987