1887

Abstract

Summary

The value of polymerase chain reaction (PCR)-based DNA fingerprinting and plasmid profile analysis for differentiation of isolates was assessed. Nineteen isolates of were typed by PCR-ribotyping, repetitive extragenic palindromic element (REP)-PCR and enterobacterial repetitive intergenic consensus (ERIC)-PCR. These methods distinguished five types by PCR-ribotyping, 11 types by REP-PCR and seven types by ERIC-PCR. The ribotyping method produced a relatively simple pattern and a small number of distinct types and was useful for differentiation of from the phenotypically similar organism, . REP- and ERIC-PCR both produced complex banding patterns, but increased the discrimination between strains. Plasmids were found in 12 of the 19 isolates and there were four different plasmid profiles. A combination of the PCR methods and plasmid profile analysis provided a high resolution typing method for

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/content/journal/jmm/10.1099/00222615-47-11-987
1998-11-01
2019-11-18
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-47-11-987
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