1887

Abstract

Summary

The polymerase chain reaction (PCR) was evaluated retrospectively for its ability to detect and spp. in respiratory tract specimens obtained from adult patients with AIDS. Mycoplasma DNA was detected in specimens from 12 of 84 patients. Of the 107 specimens tested, 13 and seven positive PCR results were obtained with the genus- and species-specific oligonucleotide primers used, respectively, in two different steps. With the latter, one sample was positive for plus , another for plus , and five others were positive for . The unexpected detection of DNA in respiratory secretions from an adult AIDS patient suggested that this urogenital mycoplasma could have a role in determining or exacerbating respiratory tract infections in the HIV-positive population, but that its low rate of isolation could be related to the frequent failure of methods used currently to detect mycoplasmas.

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/content/journal/jmm/10.1099/00222615-47-11-983
1998-11-01
2022-09-30
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References

  1. Baum S. G., Taylor-Robinson D. Mycoplasma diseases. In Mandell G. F., Bennett J. E., Dobin R. (eds) Mandeb, Douglas and Bennett’s Principles and practice of infectious diseases 4th edn New York: Churchill Fivingstone; 19951701–1718
    [Google Scholar]
  2. Heggie A. D., Jacobs M. R., Butler V. T., Baley J. E., Boxerbaum B. Frequency and significance of isolation of Ureaplasma urealyticum and Mycoplasma hominis from cerebrospinal fluid and tracheal aspirate specimens from low birth weight infants. J Pediatr 1994; 124:956–961
    [Google Scholar]
  3. Taylor-Robinson D., Furr P. M., Webster F., Webster A. D. B. Ureaplasma urealyticum in the immunocompromised host. Pediatr Infect Dis 1986; 5: (Suppl 6) S236–S238
    [Google Scholar]
  4. Skakni F., Sardet F., Just J. Detection of Mycoplasma pneumoniae in clinical samples from pediatric patients by polymerase chain reaction. J Clin Microbiol 1992; 30:2638–2643
    [Google Scholar]
  5. Hartman B., Koss M., Hui A., Baumann W., Athos F., Boylen T. Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome (AIDS). Diagnosis with bronchial brushings, biopsy, and bronchoalveolar lavage. Chest 1985; 87:603–607
    [Google Scholar]
  6. Hague R. A., Bums S. E., Mok J. Y. Q., Yap P. F. Diagnosis of Pneumocystis carinii pneumonia from non-invasive sampling of respiratory secretions. Arch Dis Child 1990; 65:1364–1365
    [Google Scholar]
  7. Kellog D. E., Kwok S. Detection of human immunodeficiency virus. In Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. (eds) PCR protocols: a guide to methods and applications San Diego: Academic Press; 1990337–347
    [Google Scholar]
  8. Kwok S., Higuchi R. Avoiding false positives with PCR. Nature 1989; 339:237–238
    [Google Scholar]
  9. Saiki R. K., Gelfand D. H., Stoffel S. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988; 239:487–491
    [Google Scholar]
  10. Roulland-Dussoix D., Henry A., Femercier B. Detection of mycoplasmas in cell cultures by PCR: a one year study. J Microbiol Methods 1994; 19:127–134
    [Google Scholar]
  11. Van Kuppeveld F. J. M., van der Fogt J. T. M., Angulo A. F. Genus-and species-specific identification of Mycoplasmas by 16S rRNA amplification. Appl Environ Microbiol 1992; 58:2606–2615
    [Google Scholar]
  12. Blanchard A. Ureaplasma urealyticum urease genes; use of a UGA tryptophan codon. Mol Microbiol 1990; 4:669–676
    [Google Scholar]
  13. Blanchard A., Hentschel J., Duffy F., Baldus K., Cassell G. H. Detection of Ureaplasma urealyticum by polymerase chain reaction in the urogenital tract of adults, in amniotic fluid, and in the respiratory tract of newborns. Clin Infect Dis 1993; 17: Suppl 1S148–153
    [Google Scholar]
  14. Teel L. D., Finelli M. R., Johnson S. C. Isolation of Mycoplasma species from bronchoalveolar lavages of patients positive and negative for human immunodeficiency virus. J Clin Microbiol 1994; 32:1387–1389
    [Google Scholar]
  15. Cassell G. H., Waites K. B., Crouse D. T. Association of Ureaplasma urealyticum infection of the lower respiratory tract with chronic lung disease and death in very-low-birth-weight infants. Lancet 1988; 2:240–244
    [Google Scholar]
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