1887

Abstract

Summary

The use of repetitive extragenic palindromic (REP)-PCR to characterise non-capsulate (NCHI) for epidemiological studies was validated by application to four outbreak-associated and three epidemiologically unrelated NCHI strains of the same phenotype which had been well characterised by other methods. The REP-PCR patterns were reproducible and showed the unrelated isolates to be distinguishable from each other, whereas the outbreak-associated isolates were indistinguishable. The results were concordant with those from outer-membrane protein enriched profiles, ribotyping and randomly amplified polymorphic DNA analysis. When applied to six further isolates from two different suspected outbreaks, rapid results were obtained from boiled supernates prepared from one colony and indicated that the isolates in question were not related. REP-PCR provides a rapid method of strain characterisation suitable for NCHI, which is ideal for use in conjunction with other methods.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-47-11-1031
1998-11-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jmm/47/11/medmicro-47-11-1031.html?itemId=/content/journal/jmm/10.1099/00222615-47-11-1031&mimeType=html&fmt=ahah

References

  1. Anderson J. R., Smith M. D., Kibbler C. C., Holton J., Scott G. M. A nosocomial outbreak due to non-encapsulated Haemophilus influenzae, analysis of plasmids coding for antibiotic resistance. J Hosp Infect 1994; 27:17–27
    [Google Scholar]
  2. Goetz M. B., O’Brien H., Musser J. M., Ward J. I. Nosocomial transmission of disease caused by nontypeable strains of Haemophilus influenzae. Am J Med 1994; 96:342–347
    [Google Scholar]
  3. Murphy T. F. Invasive H. influenzae infections in adults? Another clinical pearl down the drain!. Infect Dis Clin Pract 1994; 3:41–43
    [Google Scholar]
  4. Deulofeu F., Nava J. M., Bella F. Prospective epidemiological study of invasive Haemophilus influenzae disease in adults. Eur J Clin Microbiol Infect Dis 1994; 13:633–638
    [Google Scholar]
  5. Jordens J. Z., Leaves N. I., Anderson E. C., Slack M. P. E. Polymerase chain reaction-based strain characterization of noncapsulate Haemophilus influenzae. J Clin Microbiol 1993; 31:2981–2987
    [Google Scholar]
  6. Kostman J. R., Alden M. B., Mair M., Edlind T. D., LiPuma J. J., Stull T. L. A universal approach to bacterial molecular epidemiology by polymerase chain reaction ribotyping. J Infect Dis 1995; 171:204–208
    [Google Scholar]
  7. Jordens J. Z., Leaves N. I. Source of variation detected in ribotyping patterns of Haemophilus influenzae: comparison of traditional ribotyping, PCR-ribotyping and rDNA restriction analysis. J Med Microbiol 1997; 46:163–112
    [Google Scholar]
  8. Versalovic J., Koeuth T., Lupski J. R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 1991; 19:6823–6831
    [Google Scholar]
  9. Lessing M. P. A., Jordens J. Z., Bowler I. C. J. Molecular epidemiology of a multiple strain outbreak of methicillin-resistant Staphylococcus aureus amongst patients and staff. J Hosp Infect 1995; 31:253–260
    [Google Scholar]
  10. Jordens J. Z., Paul J., Bates J., Beaumont C., Kimari J., Gilks C. Characterisation of Streptococcus pneumoniae from human immunodeficiency virus-seropositive patients with acute and recurrent pneumonia. J Infect Dis 1995; 172:983–987
    [Google Scholar]
  11. van Belkum A., Duim B., Regelink A., Moller L., Quint W., van Alphen L. Genomic DNA fingerprinting of clinical Haemophilus influenzae isolates by polymerase chain reaction amplification: comparison with major outer-membrane protein and restriction fragment length polymorphism analysis. J Med Microbiol 1994; 41:63–68
    [Google Scholar]
  12. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol 1976; 93:9–62
    [Google Scholar]
  13. Versalovic J., Kapur V., Mason E. O. Penicillin-resistant Streptococcus pneumoniae strains recovered in Houston: identification and molecular characterization of multiple clones. J Infect Dis 1993; 167:850–856
    [Google Scholar]
  14. Meunier J.-R., Grimont P. A. D. Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res Microbiol 1993; 144:373–379
    [Google Scholar]
  15. Davin-Regli A., Abed Y., Charrel R. N., Bollet C., de Micco P. Variations in DNA concentrations significantly affect the reproducibility of RAPD fingerprint patterns. Res Microbiol 1995; 146:561–568
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-47-11-1031
Loading
/content/journal/jmm/10.1099/00222615-47-11-1031
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error