1887

Abstract

Summary

The purpose of this study was to investigate and determine the value of a novel, simple and inexpensive selective medium for isolation of yeasts of spp. – ground red hot pepper agar (GRHP). The study compared GRHP and Sabouraud dextrose agar (SDA), an insufficiently selective medium routinely used for primary isolation of yeasts. The comparison was based on qualitative and quantitative characterisation of growth of 25 bacterial strains, measurement of growth of 22 yeast strains and testing on clinical specimens. Qualitative tests on bacteria showed either significantly less growth on GRHP than on SDA, or no growth on GRHP. Quantitative tests confirmed these results; the number of colonies of all tested bacterial species and strains on GRHP was significantly lower than on SDA. With regard to the isolation of spp., GRHP had the same properties as SDA. Statistical analysis showed no significant differences in the growth of spp. and strains on the two media. All these results were confirmed by tests on clinical material. The results clearly show that GRHP agar is an economical medium for the isolation of yeasts of spp., with excellent selectivity.

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1998-11-01
2022-01-21
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References

  1. Odds F. C. Candida infections: an overview. Crit Rev Microbiol 1987; 15:1–5
    [Google Scholar]
  2. Saral R. Candida and Aspergillus infections in immunocompromised patients: an overview. Rev Infect Dis 1991; 13:487–492
    [Google Scholar]
  3. Wingard J. R. Infections due to resistant Candida species in patients with cancer who are receiving chemotherapy. Clin Infect Dis 1994; 19: Suppl 1S49–S53
    [Google Scholar]
  4. Nieto-Rodriguez J. A., Kusne S., Manez R. Factors associated with the development of candidemia and candidemia-related death among liver transplant recipients. Ann Surg 1996; 223:70–76
    [Google Scholar]
  5. Jarvis W. R. Epidemiology of nosocomial fungal infections, with emphasis on Candida species. Clin Infect Dis 1995; 20:1526–1530
    [Google Scholar]
  6. Fridkin S. K., Jarvis W. R. Epidemiology of nosocomial fungal infections. Clin Microbiol Rev 1996; 9:499–511
    [Google Scholar]
  7. Merz W. G., Sandford G., Evans G. L. Clinical evaluation of the addition of gentamicin to commercially prepared mycological media. J Clin Microbiol 1976; 3:496–500
    [Google Scholar]
  8. Mossel D. A. A., Kleynen-Semmeling A. M. C., Vincentie H. M., Beerens H., Catsaras M. Oxytetracycline-glucose-yeast extract agar for selective enumeration of moulds and yeasts in foods and clinical material. J Appl Bacteriol 1970; 33:454–457
    [Google Scholar]
  9. Bergman J. J., Berg A. O., Schneeweiss R., Heidrich F. E. Clinical comparison of microscopic and culture techniques in the diagnosis of Candida vaginitis. J Fam Pract 1984; 18:549–552
    [Google Scholar]
  10. Bernal S., Martin Mazuelos E., Garcia M., Aller A. I., Martinez M. A., Gutierrez M. J. Evaluation of CHROMagar Candida medium for the isolation and presumptive identification of species of Candida of clinical importance. Diagn Microbiol Infect Dis 1996; 24:201–204
    [Google Scholar]
  11. Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. (eds) Manual of clinical microbiology 6th edn Washington, DC: ASM Press; 1995
    [Google Scholar]
  12. Yugoslav Official Register 1979; 1: (January 5th) 21
  13. Odds F. C. Sabouraud(’s) agar. J Med Vet Mycol 1991; 29:355–359
    [Google Scholar]
  14. Cordell G. A., Araujo O. E. Capsaicin: identification, nomenclature, and pharmacotherapy. Ann Pharmacother 1993; 27:330–336
    [Google Scholar]
  15. Jones N. L., Shabib S., Sherman P. M. Capsaicin as an inhibitor of the growth of the gastric pathogen Helicobacter pylori. FEMS Microbiol Lett 1997; 146:223–227
    [Google Scholar]
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