1887

Abstract

Summary

The release of interleukin-8 (IL-8) from human neutrophils was investigated after their internalisation of the invasive bacteria and . Three pairs of bacterial strains were used to study the influence of listeriolysin O, invasiveness and Yersinia virulence plasmid (pYV)-encoded proteins on neutrophil cytokine synthesis. The neutrophils secreted IL-8 into the culture supernate after exposure to all strains tested. With , the virulence factor listeriolysin O had no effect on IL-8 secretion, whereas a non-invasive mutant induced greater IL-8 release than the corresponding invasive strain. With , expression of virulence plasmid-encoded proteins appeared to be responsible for diminished IL-8 release. Northern blot analysis showed that the induced IL-8 release was always accompanied by an enhanced level of cytoplasmic IL-8 mRNA. Cytokine priming of neutrophils with granulocyte macrophage colony-stimulating growth factor (GM-CSF), unlike granulocyte colony-stimulating factor (G-CSF), led to further enhancement of IL-8 secretion induced after internalisation of the bacteria. Again, the increased IL-8 release, from GM-CSF primed neutrophils, was accompanied by an increased level of cytoplasmic IL-8 mRNA. The results emphasise the role of IL-8 in neutrophil-mediated host defence mechanisms during the acute phase of and infection.

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/content/journal/jmm/10.1099/00222615-47-1-55
1998-01-01
2019-11-18
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