1887

Abstract

The new Sanofi Diagnostics Pasteur Chlamydia Microplate EIA shortened assay was evaluated by comparison with the original standard assay and cell culture. A total of 853 paired male and female genital tract specimens was tested with both Sanofi Chlamydia Microplate EIA shortened and standard assays and the results were compared with those of cell culture. For confirmation, a blocking assay run in the shortened format was used. Discrepancies between the three methods were resolved by a direct fluorescent antibody (DFA) test on the EIA samples or the culture retentate, or both. After resolution of discrepant results, the standard assay had a sensitivity, specificity, positive predictive value and negative predictive value of 98.5%, 100%, 100% and 99.9%, respectively. The shortened assay results were 100%, 100%, 100% and 100%, respectively. The shortened assay takes approximately 1.5 h less time than the standard assay and this study demonstrated that they have equivalent sensitivity and specificity. The improvement in turnaround time enables results to be reported on the same day.

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1997-08-01
2024-07-25
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References

  1. Homes K. K. The chlamydia epidemic. JAMA 1981; 245:1718–1723
    [Google Scholar]
  2. Schachter J. Epidemiology of Chlamydia trachomatis infections. In De la Maza L. M., Peterson E. M. (eds) Medical virology II New York: Elsevier Biomedical; 1983111–120
    [Google Scholar]
  3. Washington A. E., Johnson R. E., Sanders L. L. Chlamydia trachomatis infections in the United States. What are they costing us?. JAMA 1987; 257:2070–2072
    [Google Scholar]
  4. Washington A. E., Katz P. Cost of and payment source for pelvic inflammatory disease. Trends and projections, 1983 through 2000. JAMA 1991; 266:2565–2569
    [Google Scholar]
  5. Chemesky M. A., Jang D., Lee H. Diagnosis of Chlamydia trachomatis infections in men and women by testing first-void urine by ligase chain reaction. J Clin Microbiol 1994; 32:2682–2685
    [Google Scholar]
  6. Thejls H., Gnarpe J., Gnarpe H. Expanded gold standard in the diagnosis of Chlamydia trachomatis in a low prevalence population: Diagnostic efficacy of tissue culture, direct immunofluorescence, enzyme immunoassay, PCR and serology. Genitourin Med 1994; 70:300–303
    [Google Scholar]
  7. Moncada J., Schachter J., Bolan G. Evaluation of Syva’s enzyme immunoassay for the detection of Chlamydia trachomatis in urogenital specimens. Diag Microbiol Infect Dis 1992; 15:663–668
    [Google Scholar]
  8. Clark A., Stamm W. E., Gaydos C. Multicenter evaluation of the AntigEnz Chlamydia enzyme immunoassay for diagnosis of Chlamydia trachomatis genital infection. J Clin Microbiol 1992; 30:2762–2764
    [Google Scholar]
  9. Chan E. L., Brandt K., Kozoriz D., Mushanski L., Spence C., Horsman G. The use of a confirmation assay to increase the sensitivity and specificity of the Chlamydiazyme test. Am J Clin Pathol 1994; 102:724–728
    [Google Scholar]
  10. Chan E. L., Brandt K., Horsman G. B. A 1-year evaluation of Syva Micro Trak Chlamydia enzyme immunoassay with selective confirmation by direct fluorescent-antibody assay in a high-volume laboratory. J Clin Microbiol 1994; 32:2208–2211
    [Google Scholar]
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