1887

Abstract

The production of fatty-acid modifying enzyme (FAME) – first identified as a possible virulence factor in – has also been identified in This extracellular enzyme inactivates bactericidal fatty acids by esterifying them to cholesterol. FAME may provide protection for by inactivating these lipids present on the skin. Over 88% of 51 randomly collected isolates produced FAME; 92.2% and 13.7% of the same strains produced lipase and slime, respectively. There appeared to be no correlation of lipase activity or slime production with FAME production. The temperature optimum for FAME was between 20°C and 35°C, and the pH optimum was 6.0. Optimal enzyme activity was present at NaCl concentrations of between 250 and 500 m. FAME was not detected in culture filtrates until early stationary phase, indicating some regulatory control over enzyme production.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-46-8-693
1997-08-01
2022-08-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/46/8/medmicro-46-8-693.html?itemId=/content/journal/jmm/10.1099/00222615-46-8-693&mimeType=html&fmt=ahah

References

  1. Foster T. J. Staphylococcus. In Baron S. (ed) Medical microbiology 4th edn Galveston, TX: University of Texas Medical Branch at Galveston; 1996187–197
    [Google Scholar]
  2. Goldmann D. A., Pier G. B. Pathogenesis of infections related to intravascular cathererization. Clin Microbiol Rev 1993; 6:176–192
    [Google Scholar]
  3. Christensen G. C. The “Sticky” problem of Staphylococcus epidermidis sepsis. Hosp Pract 199327–38
    [Google Scholar]
  4. Kanai K., Kondo E. Antibactericidal and cytotoxic aspect of long-chain fatty acids as cell surface events: selected topics. Jpn J Med Sci Biol 1919; 32:135–174
    [Google Scholar]
  5. Shryock T. R., Dye E. S., Kapral F. A. The accumulation of bactericidal lipids in staphylococcal abscesses. J Med Microbiol 1992; 36:332–336
    [Google Scholar]
  6. Shryock T. R., Kapral F. A. The production of bactericidal fatty acids from glycerides in staphylococcal abscesses. J Med Microbiol 1992; 36:288–292
    [Google Scholar]
  7. Engler H. D., Kapral F. A. The production of a bactericidal monoglyceride in staphylococcal abscesses. J Med Microbiol 1992; 37:238–244
    [Google Scholar]
  8. Mortensen J. E., Shryock T. R., Kapral F. A. Modification of bactericidal fatty acids by an enzyme of Staphylococcus aureus . J Med Microbiol 1992; 36:293–298
    [Google Scholar]
  9. Kapral F. A., Smith S., Lal D. The esterification of fatty acids by Staphylococcus aureus fatty acid modifying (FAME) and its inhibition by glycerides. J Med Microbiol 1992; 37:235–237
    [Google Scholar]
  10. Long J. P., Hart J., Albers W., Kapral F. A. The production of fatty acid modifying enzyme (FAME) and lipase by various staphylococcal species. J Med Microbiol 1992; 37:232–234
    [Google Scholar]
  11. Chamberlain N. R., Imanoel B. Genetic regulation of fatty acid modifying enzyme from Staphylococcus aureus . J Med Microbiol 1996; 44:125–129
    [Google Scholar]
  12. Christensen G. D., Simpson W. A., Bisno A. L., Beachey E. H. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun 1982; 37:318–326
    [Google Scholar]
  13. Farrell A. M., Foster T. J., Holland K. T. Molecular analysis and expression of the lipase of Staphylococcus epidermidis . J Gen Microbiol 1993; 139:267–277
    [Google Scholar]
  14. Komblum J., Kreiswirth B. N., Projan S. J., Ross H., Novick R. P. Ag–r: a polycistronic locus regulating exoprotein synthesis in Staphylococcus aureus . In Novik R. P. (ed) Molecular biology of the staphylococci New York: VCH Publishers Inc; 1990373–402
    [Google Scholar]
  15. Novick R. P., Ross H. F., Projan S. J., Komblum J., Kreiswirth B., Moghazeh S. Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. EMBO J 1993; 12:3967–3975
    [Google Scholar]
  16. Balaban N., Novick R. P. Translation of RNAIII, the Staphylococcus aureus agr regulatory RNA molecule, can be activated by a 3′-end deletion. FEMS Microbiol Lett 1995; 133:155–161
    [Google Scholar]
  17. Willem J. B., Wamel V., Verhoef J., Fluit A. D.C. Cloning and characterization of an accessory gene regulator (<rgr)-locus in Staphylococcus epidermidis . In Abstracts of the 96th General Meeting of the American Society for Microbiology 1996 Washington, DC: American Society for Microbiology; Abstract B-338 p 213
    [Google Scholar]
  18. Willem J. B., Wamel V., Verhoef J., Bakker M., Fleer A., Fluit A. D.C. The correlation between virulence and delta-toxin production in Staphylococcus epidermidis . In Abstracts of the 96th General Meeting of the American Society of Microbiology 1996 Washington, DC: American Society for Microbiology; Abstract D-119 p 263
    [Google Scholar]
  19. Deighton M., Pearson S., Capstick J., Spelman D., Borland R. Phenotypic variation of Staphylococcus epidermidis isolated from a patient with native valve endocarditis. J Clin Microbiol 1992; 30:2385–2390
    [Google Scholar]
  20. Takeda S., Pier G. B., Kojima Y. Protection against endocarditis due to Staphylococcus epidermidis by immunization with capsular polysaccharide/adhesin. Circulation 1991; 84:2539–2546
    [Google Scholar]
  21. Tojo M., Yamashita N., Goldmann D. A., Pier G. B. Isolation and characterization of a capsular polysaccharide adhesin from Staphylococcus epidermidis . J Infect Dis 1988; 157:713–722
    [Google Scholar]
  22. Peters G., Locci R., Pulverer G. Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters. J Infect Dis 1982; 146:479–482
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-46-8-693
Loading
/content/journal/jmm/10.1099/00222615-46-8-693
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error