@article{mbs:/content/journal/jmm/10.1099/00222615-46-8-675, author = "Yokota, M. and Morshed, M.G. and Nakazawa, T. and Konishi, H.", title = "Protective activity of Borrelia duttonii-specific immunoglobulin subclasses in mice", journal= "Journal of Medical Microbiology", year = "1997", volume = "46", number = "8", pages = "675-680", doi = "https://doi.org/10.1099/00222615-46-8-675", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-46-8-675", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "To analyse the immune response of mice to Borrelia duttonii infection, BALB/c mice were inoculated intraperitoneally with B. duttonii strain 406K, and the titres of B. duttonii-specific immunoglobulins – IgM, IgG1, IgG2a, IgG2b and IgG3 – were determined by ELISA. IgM antibodies appeared first, followed by IgG2a and IgG3, and then IgG1 and IgG2b. The protective activity of individual classes and subclasses of B. duttonii-specific immunoglobulins was then determined by passive immunisation of BALB/c mice with immunoglobulin preparations purified by affinity chromatography. The mice were then challenged by intraperitoneal inoculation of B. duttonii. The study demonstrated that B. duttonii-specific IgM and IgG3 protected against the development of spirochaetaemia and death after borrelial infection, whereas B. duttonii-specific IgG1, IgG2a and IgG2b had low protective activities.", }