To analyse the immune response of mice to infection, BALB/c mice were inoculated intraperitoneally with strain 406K, and the titres of -specific immunoglobulins - IgM, IgG1, IgG2a, IgG2b and IgG3 - were determined by ELISA. IgM antibodies appeared first, followed by IgG2a and IgG3, and then IgG1 and IgG2b. The protective activity of individual classes and subclasses of -specific immunoglobulins was then determined by passive immunisation of BALB/c mice with immunoglobulin preparations purified by affinity chromatography. The mice were then challenged by intraperitoneal inoculation of The study demonstrated that -specific IgM and IgG3 protected against the development of spirochaetaemia and death after borrelial infection, whereas -specific IgG1, IgG2a and IgG2b had low protective activities.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error