1887

Abstract

A questionnaire was sent to the 48 national typing centres for and 31 replies were received. Methods of phage typing varied and molecular methods were not universally available, although pulsed-field gel electrophoresis was offered by 13 centres. Results for a quality control phage typing exercise were received from 25 centres. Increased standardisation of methods and definitions are indicated. Differences from the consensus patterns were mainly due to typing at an inappropriate dilution of phage, but five strains caused difficulties in many centres. Overall reproducibility was good. Phage typing remains a cost-effective method for epidemiological studies, particularly on a large scale. The strains selected for the quality control exercise included many strains suitable for controlling molecular methods as well as testing phage typing. Molecular methods help in the validation of the conclusions which may be drawn from phage typing.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-46-6-511
1997-06-01
2022-09-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/46/6/medmicro-46-6-511.html?itemId=/content/journal/jmm/10.1099/00222615-46-6-511&mimeType=html&fmt=ahah

References

  1. Blair J. E., Williams R. E. O. Phage typing of staphylococci. Bull World Health Organ 1961; 24:771–784
    [Google Scholar]
  2. Parker M. T. Phage-typing of Staphylococcus aureus. In Norris J. R., Ribbons D. W. (eds) Methods in microbiology vol 7B London: Academic Press; 19721–28
    [Google Scholar]
  3. Richardson J. F., Noble W. C., Marples R. R. Species identification and epidemiological typing of the staphylococci. In Board R. G., Jones D., Skinner F. A. (eds) Identification methods in applied and environmental microbiology. Society for Applied Bacteriology Technical Series no. 29 Oxford: Blackwell Scientific Publications; 1992193–219
    [Google Scholar]
  4. Asheshov E. H., Skalova R. International Committee on Systematic Bacteriology. Subcommittee on the phage-typing of staphylococci. Minutes of the meeting, 26 September 1974. Int J Syst Bacteriol 1975; 25:233–234
    [Google Scholar]
  5. Marples R. R. Extraordinary meeting of the International Union of Microbiological Societies Subcommittee on the phagetyping of Staphylococci. Minutes of the meeting, 30 June 1992. Int J Syst Bacteriol 1993; 43:187
    [Google Scholar]
  6. Cookson B. D., Aparicio P., Deplano A., Struelens M., Goering R., Marples R. Inter-centre comparison of pulsed-field gel electrophoresis for the typing of methicillin-resistant Staphylococcus aureus. J Med Microbiol 1996; 44:179–184
    [Google Scholar]
  7. Dave J., Reith S., Nash J. Q., Marples R. R., Dulake C. A double outbreak of exfoliative toxin-producing strains of Staphylococcus aureus in a maternity unit. Epidemiol Infect 1994; 112:103–114
    [Google Scholar]
  8. Williams R. E. O., Rippon J. E. Bacteriophage typing of Staphylococcus aureus. J Hyg 1952; 50:320–353
    [Google Scholar]
  9. Tenover F. C., Arbeit R., Archer G. Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. J Clin Microbiol 1994; 32:407–415
    [Google Scholar]
  10. Bannerman T. L., Hancock G. A., Tenover F. C., Miller J. M. Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus. J Clin Microbiol 1995; 33:551–555
    [Google Scholar]
  11. Anon Epidemic methicillin resistant Staphylococcus aureus. Commun Dis Rep CDR Weekly 1996; 6:197
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-46-6-511
Loading
/content/journal/jmm/10.1099/00222615-46-6-511
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error