Genetic Differentiation of Australian Isolates of by Pulsed-Field Gel Electrophoresis Free

Abstract

As part of an epidemiological study of tuberculosis in Australia, 84 isolates of from patients were analysed by pulsed-field gel electrophoresis (PFGE). The isolates were genetically heterogeneous, with 66 different DNA banding patterns obtained following digestion of genomic DNA with 1 and 53 patterns with 1. When the results were compared with those previously obtained in restriction fragment length polymorphism analysis (RFLP), in 87% of cases the results with 1 were consistent with those obtained with insertion sequence IS6110 as a probe in RFLP. However, PFGE was able to differentiate four of eight isolates which were identical with IS6110 typing. The high polymorphism amongst strains and the high average age of the patients (51 years) suggested that most organisms were cultured from patients who had reactivation of existing infections. Isolates with identical DNA patterns were found in different states of Australia, but no one strain predominated in any area. This suggests that tuberculosis has been introduced into Australia from various sources.

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1997-06-01
2024-03-29
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References

  1. Dolin P. J., Raviglione M. C., Kochi A. Global tuberculosis incidence and mortality during 1990-2000. Bull World Health Organ 1994; 72:213–220
    [Google Scholar]
  2. Hargreaves J. Tuberculosis notifications in Australia, 1992. Comm Dis Intel 1994; 18:330–339
    [Google Scholar]
  3. Dawson D. J., Cheah D. F., Chew W. K., Haverkort F. C., Lumb R., Sievers A. S. Tuberculosis in Australia, 1989-1992. Bacteriologically confirmed cases and drug resistance. Med J Aust 1995; 162:287–290
    [Google Scholar]
  4. Cheah D. Tuberculosis notification rates, Australia, 1991. Comm Dis Intel 1991; 16:389–400
    [Google Scholar]
  5. Dwyer B., Jackson K., Raios K., Sievers A., Wilshire E., Ross B. DNA restriction fragment analysis to define an extended cluster of tuberculosis in homeless men and their associates. J Infect Dis 1993; 167:490–494
    [Google Scholar]
  6. Oliver G. Tuberculosis notification in Australia. Comm Dis Intel 1996; 20:108–115
    [Google Scholar]
  7. Alperstein G., Fett M. J., Reznik R., Thomas M., Senthil M. The prevalence of tuberculosis infection among year 8 schoolchildren in inner Sydney in 1992. Med J Aust 1994; 160:197–201
    [Google Scholar]
  8. Small P. M., van Embden J. D. A. Molecular epidemiology of tuberculosis. In Bloom B. R. (ed) Tuberculosis: pathogenesis, protection, and control Washington DC: ASM Press; 1994569–582
    [Google Scholar]
  9. Jones W. D., Good R. C., Thompson N. J., Kelly G. D. Bacteriophage types of Mycobacterium tuberculosis in the United States. Am Rev Respir Dis 1982; 125:640–643
    [Google Scholar]
  10. Jones W. D., Woodley C. L. Phage-type patterns of Mycobacterium tuberculosis from Southeast Asian immigrants. Am Rev Respir Dis 1983; 127:348–349
    [Google Scholar]
  11. Snider D. E., Jones W. D., Good R. C. The usefulness of phage typing Mycobacterium tuberculosis isolates. Am Rev Respir Dis 1984; 130:1095–1099
    [Google Scholar]
  12. Maslow J. N., Mulligan M. E., Arbeit R. D. Molecular epidemiology: application of contemporary techniques to the typing of microorganisms. Clin Infect Dis 1993; 17:153–164
    [Google Scholar]
  13. Drobniewski F. A., Kent R. J., Stoker N. G., Uttley A. H. C. Molecular biology in the diagnosis and epidemiology of tuberculosis. J Hasp Infect 1994; 28:249–263
    [Google Scholar]
  14. Collins D. M., de Lisle G. W. DNA restriction endonuclease analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG. J Gen Microbiol 1984; 130:1019–1021
    [Google Scholar]
  15. Collins D. M., de Lisle G. W. DNA restriction endonucleases analysis of Mycobacterium bovis and other members of the tuberculosis complex. J Clin Microbiol 1985; 21:562–564
    [Google Scholar]
  16. Edlin B. R., Tokars J. I., Grieco M. H. An outbreak of multidrug-resistant tuberculosis among hospitalized patients with the acquired immunodeficiency syndrome. New Engl J Med 1992; 326:1514–1521
    [Google Scholar]
  17. Yuen L. K. W., Ross B. C., Jackson K. M., Dwyer B. Characterisation of Mycobacterium tuberculosis strains from Vietnamese patients by Southern blot hybridization. J Clin Microbiol 1993; 31:1615–1618
    [Google Scholar]
  18. Bono M., Jemmi T., Bemasconi C., Burki D., Telenti A., Bodmer T. Genotypic characterization of Mycobacterium avium strains recovered from animals and their comparison to human strains. Appl Environ Microbiol 1995; 61:371–373
    [Google Scholar]
  19. Guerrero C., Bemasconi C., Burki D., Bodmer T., Telenti A. A novel insertion element from Mycobacterium avium, IS 1245, is a specific target for analysis of strain relatedness. J Clin Microbiol 1995; 33:304–307
    [Google Scholar]
  20. Tenover F. C., Arbeit R. D., Goering R. V. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33:2233–2239
    [Google Scholar]
  21. Zhang Y., Mazurek G. H., Kave M. D. DNA polymorphisms in strains of Mycobacterium tuberculosis analyzed by pulsed-field gel electrophoresis: a tool for epidemiology. J Clin Microbiol 1992; 30:1551–1556
    [Google Scholar]
  22. Olson E. S., Forbes K. J., Watt B., Pennington T. H. Population genetics of Mycobacterium tuberculosis complex in Scotland analysed by pulsed-field gel electrophoresis. Epidemiol Infect 1995; 114:153–160
    [Google Scholar]
  23. Ross B. C., Raios K., Jackson K., Dwyer B. Molecular cloning of a highly repeated DNA element from Mycobacterium tuberculosis and its use as an epidemiological tool. J Clin Microbiol 1992; 30:942–946
    [Google Scholar]
  24. Feizabadi M. M., Robertson I. D., Cousins D. Y., Hampson D. J. Genomic analysis of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex by isoenzyme analysis and pulsed-field gel electrophoresis. J Clin Microbiol 1996; 34:1136–1142
    [Google Scholar]
  25. Yang Z. H., Mtoni I., Chondi M. DNA fingerprinting and phenotyping of Mycobacterium tuberculosis isolates from human immunodeficiency virus (HlV)-seropositive and HIV-seronegative patients in Tanzania. J Clin Microbiol 1995; 33:1064–1069
    [Google Scholar]
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