1887

Abstract

A massive accumulation of neutrophils, mainly due to enhanced interleukin-8 (IL-8) levels, is believed to contribute to the deleterious effects of lung infection, e.g., in cystic fibrosis (CF). Antibodies to phospholipase C, an exoenzyme of , are detected early and at high levels in CF patients. However, produces at least two types of phospholipase C (PLC), one haemolytic (PLC-H) and the other non-haemolytic (PLC-N), both with mol. wts of . 77 kDa. Experiments were performed to evaluate the potential contribution of PLC to neutrophil accumulation during infection. Therefore, PLC-H and PLC-N were compared with regard to IL-8 generation from human monocytes. Purified PLC-H as well as culture supernates (mol. wt > 50 kDa) of a strain capable of producing both PLC-H and PLC-N, and mutant strains deficient in the production of one or other phospholipase, or both, were examined. Purified PLC-H (only at low concentrations up to 1 unit/4 × 10monocytes), induced a dose-dependent increase in IL-8 release and IL-8-specific mRNA expression over that of unstimulated cells (at 4-, 12- and 24-h incubation times). Higher concentrations of PLC-H led to a decrease in IL-8 release and IL-8-specific mRNA expression. These findings were confirmed by the results obtained with the supernates of cultures of mutant strains of PAO1 that produced either a PLC-H or PLC-N or neither. Stimulation and inhibition of IL-8 release and mRNA expression were associated with a culture supernate fraction of mol. wt > 50 kDa and containing PLC-H. These results contribute to the understanding of the role of both PLC in IL-8 generation during their interaction with human monocytes.

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1997-06-01
2022-12-02
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References

  1. Baltimore R. S., Christie C. D., Smith G. J. Immunohistopathologic localization of Pseudomonas aeruginosa in lungs from patients with cystic fibrosis. Implications for the pathogenesis of progressive lung deterioration. Am Rev Respir Dis 1989; 140:1650–1661
    [Google Scholar]
  2. Bergmann U., Scheffer J., Roller M. Induction of inflammatory mediators (histamine and leukotrienes) from rat peritoneal mast cells and human granulocytes by Pseudomonas aeruginosa strains from bum patients. Infect Immun 1989; 57:2187–2195
    [Google Scholar]
  3. Döring G. Chronic Pseudomonas aeruginosa lung infection in cystic fibrosis patients. In Campa M., Bendinelli M., Friedman H. (eds) Pseudomonas aeruginosa as an opportunistic pathogen New York, NY: Plenum Press; 1993245–273
    [Google Scholar]
  4. Hoiby N. Cystic fibrosis infection. Schweiz Med Wochenschr 1991; 121:105–109
    [Google Scholar]
  5. Pier G. B. Pulmonary disease associated with Pseudomonas aeruginosa in cystic fibrosis: current status of the host-bacterium interaction. J Infect Dis 1985; 151:575–580
    [Google Scholar]
  6. Fick R. B., Standiford T. J., Kunkel S. L., Strieter R. M. Interleukin-8 (IL-8) and neutrophil accumulation in the inflammatory airways disease of cystic fibrosis (CF). Clin Res 1991; 39: 292A (Abstract)
    [Google Scholar]
  7. Wiener-Kronish J. P., Sakuma T., Kudoh I. Alveolar epithelial injury and pleural empyema in acute P aeruginosa pneumonia in anaesthetized rabbits. J Appl Physiol 1993; 75:1661–1669
    [Google Scholar]
  8. Bonfield T. L., Ghnaim H. A., Panuska J. R., Konstan M., Berger M. Cytokines in the lungs of cystic fibrosis patients. FASEB Research Summer Conference; Vermont: 1993
    [Google Scholar]
  9. Konstan M. W., Walenga R. W., Hiliard K. A., Berger M. Eicosanoid content of bronchoalveolar lavage fluid is markedly elevated in cystic fibrosis. Pediatr Pulm 1991; 6:302–309
    [Google Scholar]
  10. Willems J., Joniau M., Cinque S., van Damme J. Human granulocyte chemotactic peptide (IL-8) as a specific neutrophil degranulator; comparison with other monokines. Immunology 1989; 67:540–542
    [Google Scholar]
  11. Berka R. M., Gray G. L., Vasil M. L. Studies on phospholipase C (heat-labile hemolysin) in Pseudomonas aeruginosa. Infect Immun 1981; 34:1071–1074
    [Google Scholar]
  12. Meyers D. J., Berk R. S. Characterization of phospholipase C from Pseudomonas aeruginosa as a potent inflammatory agent. Infect Immun 1990; 58:659–666
    [Google Scholar]
  13. Vasil M. L., Graham L. M., Ostroff R. M., Shortridge V. D., Vasil A. L. Phospholipase C: molecular biology and contribution to the pathogenesis of Pseudomonas aeruginosa. In Homma J. Y., Tanimoto H., Holder I. A., Hoiby N., Döring G. (eds) Pseudomonas aeruginosa in human diseases Antibiotics and Chemotherapy vol 44 Basel: Karger; 199134–47
    [Google Scholar]
  14. Granström M., Ericsson A., Strandvik B. Relation between antibody response to Pseudomonas aeruginosa exoproteins and colonization/infection in patients with cystic fibrosis. Acta Paediatr Scand 1984; 73:772–777
    [Google Scholar]
  15. Hollsing A. E., Granström M., Vasil M. L., Wretlind B., Strandvik B. Prospective study of serum antibodies to Pseudomonas aeruginosa exoproteins in cystic fibrosis. J Clin Microbiol 1987; 25:1868–1874
    [Google Scholar]
  16. Ostroff R. M., Wretlind B., Vasil M. L. Mutations in the hemolytic-phospholipase C operon result in decreased virulence of Pseudomonas aeruginosa PAO1 grown under phosphate-limiting conditions. Infect Immun 1989; 57:1369–1373
    [Google Scholar]
  17. Ostroff R. M., Vasil A. L., Vasil M. L. Molecular comparison of a nonhemolytic and a hemolytic phospholipase C from Pseudomonas aeruginosa. J Bacteriol 1990; 172:5915–5923
    [Google Scholar]
  18. Shortridge V. D., Lazdunski A., Vasil M. L. Osmoprotectants and phosphate regulate expression of phospholipase C in Pseudomonas aeruginosa. Mol Microbiol 1992; 6:863–871
    [Google Scholar]
  19. Graham L. M., Vasil A. L., Vasil M. L., Voelkel N. F., Stenmark K. R. Decreased pulmonary vasoreactivity in an animal model of chronic Pseudomonas pneumonia. Am Rev Respir Dis 1990; 142:221–229
    [Google Scholar]
  20. König B., Jaeger K.-E., König W. Induction of inflammatory mediator release (12-hydroxyeicosatetraenoic acid) from human platelets by Pseudomonas aeruginosa. Int Arch Allergy Immunol 1994; 104:33–41
    [Google Scholar]
  21. Brunschwig E., Darzins A. A two-component T7 system for the overexpression of genes in Pseudomonas aeruginosa. Gene 1992; 111:35–41
    [Google Scholar]
  22. Boyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one step centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl 97 196877–78
    [Google Scholar]
  23. Tucker S. B., Pierre R. V., Jordon R. E. Rapid identification of monocytes in a mixed mononuclear cell preparation. J Immunol Methods 1977; 14:267–269
    [Google Scholar]
  24. König B., Ceska M., Konig W. Effect of Pseudomonas aeruginosa on interleukin-8 release from human phagocytes. Int Arch Allergy Immunol 1995; 106:357–365
    [Google Scholar]
  25. Kindt G. C., Gadek J. E., Weiland J. E. Initial recruitment of neutrophils to alveolar structures in acute lung injury. J Appl Physiol 1991; 70:1575–1585
    [Google Scholar]
  26. König W., Schonfeld W., Raulf M. The neutrophil and leukotrienes – role in health and disease. Eicosanoids 1990; 3:1–22
    [Google Scholar]
  27. Baggiolini M., Clark-Lewis I. Interleukin-8, a chemotactic and inflammatory cytokine. FEBS Lett 1992; 307:97–101
    [Google Scholar]
  28. König B., König W. Induction and suppression of cytokine release (tumour necrosis factor-alpha; interleukin-6; interleukin 1β) by Escherichia coli pathogenicity factors (adhesins, alpha-haemolysin). Immunology 1993; 78:526–533
    [Google Scholar]
  29. Massion P. P., Inoue H., Richman-Eisenstat J. Novel Pseudomonas product stimulates interleukin-8 production in airway epithelial cells in vitro. J Clin Invest 1994; 93:26–32
    [Google Scholar]
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