1887

Abstract

The 16S ribosomal RNA (rRNA) gene of the phylogenetic subdivision containing gram-positive bacteria with a high G + C content was detected specifically in clinical specimens from patients suspected of having Whipple’s disease. The primary structure of 16S rDNA amplified from clinical samples was determined by cloning and sequencing. Two sorts of sequences were identified: one corresponded exactly to the rRNA sequence of (GenBank accession no. M87484) while the other was related to that of members of the genus No sequence related to spp. or was observed. Exhaustive examination of negative specimens with broad-range eubacterial primers detected one sequence related to Enterobacter-iaceae and another related to spp. To speed identification of , a nested amplification method was devised. A first amplification specific for the gram-positive bacteria subdivision was performed, followed by a second amplification with -specific primers. The amplified product was checked by digestion with II, I, and I endonucleases. These techniques were applied to DNA extracted from seven intestinal biopsy samples, two cerebrospinal fluid samples and one articular fluid from patients suspected of having Whipple’s disease. 16S rDNA was found in two of the biopsy samples, one of the cerebrospinal fluid samples and in the articular fluid.

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1997-04-01
2024-12-12
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References

  1. Dobbins W. O. Whipple’s disease. Springfield: Charles C Thomas; 1987
    [Google Scholar]
  2. Maizel H., Ruffin J. M., Dobbins W. O. Whipple’s disease: a review of 19 patients from one hospital and a review of the literature since 1950. Medicine (Baltimore) 1970; 49:175–205
    [Google Scholar]
  3. Feurle G. E., Volk B., Waldherr R. Cerebral Whipple’s disease with negative jejunal histology. N Engl J Med 1979; 300:907–908
    [Google Scholar]
  4. Reiman A. D. Whipple’s disease. In Blaser M. J., Smith P. D., Ravdin J. I., Greenberg H. B., Guerrant R. L. (eds) Infections of the gastrointestinal tract New York: Raven Press; 1995919–936
    [Google Scholar]
  5. Black-Schaffer B. The tinctorial demonstration of a glycoprotein in Whipple’s disease. Proc Soc Exp Biol Med 1949; 72:225–227
    [Google Scholar]
  6. Sieracki J. C., Fine G. Whipple’s disease: observations on systemic involvement. II. Gross and histologic observations. Arch Pathol 1959; 67:81–93
    [Google Scholar]
  7. Silva M. T., Macedo P. M., Nunes J. F.M. Ultrastructure of bacilli and the bacillary origin of the macrophagic inclusions in Whipple’s disease. J Gen Microbiol 1985; 131:1001–1013
    [Google Scholar]
  8. Strom R. L., Gruninger R. P. AIDS with Mycobacterium avium-intracellulare lesions resembling those of Whipple’s disease. N Engl J Med 1983; 309:1323–1324
    [Google Scholar]
  9. Roth R. I., Owen R. L., Keren D. F. AIDS with Mycobacterium avium-intracellulare lesions resembling those of Whipple’s disease. N Engl J Med 1983; 309:1324–1325
    [Google Scholar]
  10. Wang H. H., Tollerud D., Danar D., Hanff P., Gottesdiener K., Rosen S. Another Whipple-like disease in AIDS?. N Engl J Med 1986; 314:1577–1578
    [Google Scholar]
  11. Upton A. C. Histochemical investigation of the mesenchymal lesions in Whipple’s disease. Am J Clin Pathol 1952; 22:755–764
    [Google Scholar]
  12. Rodarte J. R., Garrison C. O., Holley K. E., Fontana R. S. Whipple’s disease simulating sarcoidosis. A case with unique clinical and histological features. Arch Int Med 1972; 129:479–482
    [Google Scholar]
  13. Hawkins C. F., Farr M., Morris C. J., Hoare A. M., Williamson N. Detection by electron microscope of rod-shaped organisms in synovial membrane from a patient with the arthritis of Whipple’s disease. Ann Rheum Dis 1976; 35:502–509
    [Google Scholar]
  14. Dobbins W. O. The diagnosis of Whipple’s disease. N Engl J Med 1995; 332:390–392
    [Google Scholar]
  15. Meier J., Berwanger I., Zangana N., Hellerich O. [Whipple disease with negative duodenal histology – a case report and review of the literature.]. Z Rheumatol 1994; 53:357–362
    [Google Scholar]
  16. Rickman L. S., Freeman W. R., Green W. R. Uveitis caused by Tropheryma whippelii (Whipple’s bacillus). N Engl J Med 1995; 332:363–366
    [Google Scholar]
  17. Fantry G. T., James S. P. Whipple’s disease. Dig Dis 1995; 13:108–118
    [Google Scholar]
  18. Adams M., Rhyner P. A., Day J., Dearmond S., Smuckler E. A. Whipple’s disease confined to the central nervous system. Ann Neurol 1987; 21:104–108
    [Google Scholar]
  19. Yardley J. H., Hendrix T. R. Combined electron and light microscopy in Whipple’s disease. Demonstration of ‘bacillary bodies’ in the intestine. Bull John Hopk Hosp 1961; 109:80
    [Google Scholar]
  20. Crockett Chears W., Ashworth C. T. Electron microscopic study of the intestinal mucosa in Whipple’s disease. Demonstration of encapsulated bacilliform bodies in the lesion. Gastroenterology 1961; 41:129–138
    [Google Scholar]
  21. Chen K., Neimark H., Rumore P., Steinman C. R. Broad range DNA probes for detecting and amplifying eubacterial nucleic acids. FEMS Microbiol Lett 1989; 57:19–24
    [Google Scholar]
  22. Wilson K. H., Blitchington R., Frothingham R., Wilson J. A.P. Phylogeny of the Whipple’s-disease-associated bacterium. Lancet 1991; 338:474–475
    [Google Scholar]
  23. Reiman A. D., Schmidt T. M., MacDermott R. P., Falkow S. Identification of the uncultured bacillus of Whipple’s disease. N Engl J Med 1992; 327:293–301
    [Google Scholar]
  24. Muller C., Stain C., Burghuber O. Tropheryma whippelii in peripheral blood mononuclear cells and cells of pleural effusion. Lancet 1993; 341:701
    [Google Scholar]
  25. von Herbay A., Ditton H. J., Maiwald M., Meier-Willersen H. J. Nachweis von Tropheryma whippelii mit der Polymerase-Kettenreaktion vor und nach Therapie eines Morbus Whipple. [Detection of Tropheryma whippelii using the polymerase chain reaction before and after therapy of Whipple’s disease.]. Dtsch Med Wochenschr 1994; 119:1679
    [Google Scholar]
  26. Riederer J. Alterer patient mit Arthralgien, Fieberschiiben, Schwache und Gewichtsabnahme. [An elderly patient with arthralgia, fever, weakness and weight loss. Diagnosis of Whipple’s disease by PCR detection of Tropheryma whippelii.]. Fortschr Med 1995; 113:70–72
    [Google Scholar]
  27. Maiwald M., Meier-Willersen H. J., Hartmann M., von Herbay A. Detection of Tropheryma whippelii DNA in a patient with AIDS. J Clin Microbiol 1995; 33:1354–1356
    [Google Scholar]
  28. Lowsky R., Archer G. L., Fyles G. Diagnosis of Whipple’s disease by molecular analysis of peripheral blood. N Engl J Med 1994; 331:1343–1346
    [Google Scholar]
  29. Wendler D., Mendoza E., Schleiffer T., Zander M., Maier M. Tropheryma whippelii endocarditis confirmed by polymerase chain reaction. Eur Eleart J 1995; 16:424–425
    [Google Scholar]
  30. Woese C. R. Bacterial evolution. Microbiol Rev 1987; 51:221–271
    [Google Scholar]
  31. Fresard A., Guglielminotti C., Berthelot P. Prosthetic joint infection caused by Tropheryma whippelii (Whipple’s bacillus). Clin Infect Dis 1996; 22:575–576
    [Google Scholar]
  32. Cohen L., Berthet K., Dauga C., Thivart L., Pierrot-Deseilligny C. Polymerase chain reaction of cerebrospinal fluid to diagnose Whipple’s disease. Lancet 1996; 347:329
    [Google Scholar]
  33. Dauga C., Miras I., Grimont P. A.D. Identification of Bartonella henselae and B. quintana 16SrDNA sequences by branch-, genus- and species-specific amplification. J Med Microbiol 1996; 45:192–199
    [Google Scholar]
  34. Sarkar G., Sommer S. S. Shedding light on PCR contamination. Nature 1990; 343:27
    [Google Scholar]
  35. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. Complete nucleotide sequence of 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci USA 1978; 75:4801–4805
    [Google Scholar]
  36. Bottger E. C. Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA. FEMS Microbiol Lett 1989; 65:171–176
    [Google Scholar]
  37. Rashtchian A., Buchman G. W., Schuster D. M., Berninger M. S. Uracil DNA glycosylase-mediated cloning of polymerase chain reaction-amplified DNA: application to genomic and cDNA cloning. Anal Biochem 1992; 206:91–97
    [Google Scholar]
  38. Maniatis T., Sambrook J., Fritsch E. F. (eds) Molecular cloning: a laboratory manual 2nd edn Cold Spring Harbor, New York: Cold Spring Harbor Laboratory; 1989
    [Google Scholar]
  39. Blakesley R. W. Cycle sequencing. In Griffin H., Griffin A. (eds) Methods in molecular biology, DNA sequencing protocols Totowa, NJ: Humana Press Inc; 199323
    [Google Scholar]
  40. Swofford D. PAUP: phylogenetic analysis using parsimony, version 3.0. Computer program distributed by the Illinois Natural History Survey; Champaign, Illinois, USA: 1990
    [Google Scholar]
  41. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120
    [Google Scholar]
  42. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
    [Google Scholar]
  43. Felsenstein J. PHYLIP – Phylogeny Inference Package (version 3.2). Cladistics 1989; 5:164–166
    [Google Scholar]
  44. Ruimy R., Riegel P., Boiron P., Monteil H., Christen R. Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences. Int J Syst Bacteriol 1995; 45:740–746
    [Google Scholar]
  45. Funke G., Lawson P. A., Bernard K. A., Collins M. D. Most Corynebacterium xerosis strains identified in the routine clinical laboratory correspond to Corynebacterium amycolatum . J Clin Microbiol 1996; 34:1124–1128
    [Google Scholar]
  46. Gupta S., Pinching A. J., Onwubalili J., Vince A., Evans D. J., Hogson H. J.F. Whipple’s disease with unusual clinical, bacteriologic, and immunologic findings. Gastroenterology 1986; 90:1286–1289
    [Google Scholar]
  47. Ryser R. J., Locksley R. M., Eng S. C., Dobbins W. O., Schoenknecht R. D., Rubin C. E. Reversal of dementia associated with Whipple’s disease by trimethoprim-sulfamethoxazole, drugs that penetrate the blood-brain barrier. Gastroenterology 1984; 86:745–752
    [Google Scholar]
  48. Knox D. L., Bayless T. M., Pittman F. E. Neurologic disease in patients with treated Whipple’s disease. Medicine 1976; 55:467–476
    [Google Scholar]
  49. Tan T. Q., Vogel H., Tharp B. R., Carrol C. L., Kaplan S. L. Presumed central nervous system Whipple’s disease in a child: case report. Clin Infect Dis 1995; 20:883–889
    [Google Scholar]
  50. Reiman D. A., Loutit J. S., Schmidt T. M., Falkow S., Tompkins L. S. The agent of bacillary angiomatosis. An approach to the identification of uncultured pathogens. N Engl J Med 1990; 323:1573–1580
    [Google Scholar]
  51. Boddinghaus B., Rogall T., Flohr T., Blocker H., Bottger E. Detection and identification of mycobacteria by amplification of rRNA. J Clin Microbiol 1990; 28:1751–1759
    [Google Scholar]
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