1887

Abstract

The susceptibility of male Swiss white mice (MF1) to -induced arthritis was investigated with wild-type strain allelic replacement mutants. Comparison was made with a known mouse arthritogenic strain. The development and severity of arthritis were dependent both on the numbers of live bacteria injected intravenously and also on the mutant used; the ID50 ranged from (5 X 10) – (1 X 10) cfu. The results indicate that expression of the genes associated with virulence, including those for protein A and -haemolysin, play a major role in the pathogenesis of staphylococcal septic arthritis. When either virulence component was carried by the variant, a greater degree of inflammation, pannus formation and cartilage destruction was detected histologically. Loss of one or more virulence factors lowered the septic arthritis severity score based on clinical and histological parameters.

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1997-03-01
2024-12-04
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References

  1. Kellgren J. H., Ball J., Fairbrother R. W., Barnes K. L. Suppurating arthritis complicating rheumatoid arthritis. BMJ 1958; 1:1193–1200
    [Google Scholar]
  2. Mitchell M., Howard B., Haller J., Sartoris D. J., Resnick D. Septic arthritis. Radiol Clin North Am 1988; 26:1295–1313
    [Google Scholar]
  3. Goldenberg D. L. Septic arthritis in chronic joint disease. Rheumatology Review 1992; 1:147–149
    [Google Scholar]
  4. Mikhail I. S., Alarcon G. S. Nongonococcal bacterial arthritis. Rheum Dis Clin North Am 1993; 19:311–331
    [Google Scholar]
  5. Bremell T., Lange S., Yacoub A., Rydén C., Tarkowski A. Experimental Staphylococcus aureus arthritis in mice. Infect Immun 1991; 59:2615–2623
    [Google Scholar]
  6. Riegels-Nielsen P., Frimodt-Moller N., Jensen J. S. Rabbit model of septic arthritis. Acta Orthop Scand 1987; 58:14–19
    [Google Scholar]
  7. Bremell T., Abdelnour A., Tarkowski A. Histopathological and serological progression of experimental Staphylococcus aureus arthritis. Infect Immun 1992; 60:2976–2985
    [Google Scholar]
  8. Sturrock R. D., Giffard C. J., Gemmell C. G., Reid R. Staphylococcus aureus infection in mice: a model for septic arthritis. Clin Rheumatol 1993; 12:35
    [Google Scholar]
  9. Abdelnour A., Arvidson S., Bremell T., Rydén C., Tarkowski A. The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine arthritis model. Infect Immun 1993; 61:3879–3885
    [Google Scholar]
  10. Goutcher S., Reid R., Sturrock R. D., Gemmell C. G. Influence of virulence factors of Staphylococcus aureus in the development of septic arthritis in a mouse model. Br J Rheumatol 1994; 33: Abstracts Suppl: (S2) 3
    [Google Scholar]
  11. O’Reilly M., de Azavedo J. C. S., Kennedy S., Foster T. J. Inactivation of alpha-haemolysin gene of Staphylococcus aureus 8325-4 by site-directed mutagenesis and studies on the expression of its haemolysins. Microb Pathog 1986; 1:128–138
    [Google Scholar]
  12. Kornblum J., Kreiswirth B. N., Projan S. J., Ross H., Novick R. P. Agr: a polycistronic locus regulating exoprotein synthesis in Staphylococcus aureus . In Novick R. P., Skurray P. (eds) The molecular biology of staphylococci New York: VCH Publishers; 1990373–402
    [Google Scholar]
  13. Gemmell C. G., Tree R., Patel A., O’Reilly M., Foster T. J. Susceptibility to opsonophagocytosis of protein A, α-haemolysin and β-toxin deficient mutants of S. aureus isolated by allele relacement. In Jeljaszewicz J., Ciborowski P. (eds) The staphylococci (Zentralbl Balcteriol Suppl 21) Stuttgart: Gustav Fischer; 1991273–277
    [Google Scholar]
  14. Rydén C., Yacoub A. I., Maxe D. Specific binding of bone sialoprotein to Staphylococcus aureus isolated from patients with osteomyelitis. Eur J Biochem 1989; 184:331–336
    [Google Scholar]
  15. Tissi L., Marconi P., Mosci P. Experimental model of type IV Streptococcus agalactiae (group B Streptococcus) infection in mice with early development of septic arthritis. Infect Immun 1990; 58:3093–3100
    [Google Scholar]
  16. Patel A. H., Nowlan P., Weavers E. D., Foster T. Virulence of protein A-deficient and alpha-toxin-deficient mutants of Staphylococcus aureus isolated by allelle replacement. Infect Immun 1987; 55:3103–3110
    [Google Scholar]
  17. Recsei P., Kreiswirth B., O’Reilly M., Schlievert P., Gruss A., Novick R. P. Regulation of exoprotein gene expression in Staphylococcus aureus by agr. Mol Gen Genet 1986; 202:58–61
    [Google Scholar]
  18. Hurst N. P., Nuki G. Evidence for defect of complement-mediated phagocytosis by monocytes from patients with rheumatoid arthritis and cutaneous vasculitis. BMJ 1987; 282:2081–2083
    [Google Scholar]
  19. King S. E., Parker J., Cooper R., Sturrock R. D., Gemmell C. G. Polymorphonuclear leucocyte function in rheumatoid arthritis. Br J Rheumatol 1986; 25:26–33
    [Google Scholar]
  20. Goulding N. J., Guyre P. M. Impairment of neutrophil Fcγ receptor mediated transmembrane signalling in active rheumatoid arthritis. Ann Rheum Dis 1992; 51:594–599
    [Google Scholar]
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