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Volume 46, Issue 2

Characterisation and protective capacity of monoclonal antibodies elicited in mice against protein epitopes of antibiotic-exposed Free

Abstract

The binding capacity and the protective activity of three monoclonal antibodies (MAbs) – ARM 1-4, ARM 1-7 and ARM 2-2 – obtained from spleen cells of mice immunised with O6:K– pre-treated with sub-MIC of aztreonam were studied. The MAbs belonged to IgG isotype and showed different reactivity toward protein epitopes of in an immunoblotting assay. ARM 1-4 recognised epitopes on molecules of 30 kDa and 40 kDa. ARM 1-7 identified an epitope of a molecule of 41 kDa, and ARM 2-2 recognised epitopes of molecules of 15 kDa and 41 kDa. In ELISA the MAbs cross-reacted with O7:K–, O111:B4 and O128:K– with different binding affinity. Furthermore, the MAbs showed complement-dependent bactericidal activity. The MAbs displayed different protective capacities when given to mice 90 min before lethal challenges with 2 × LD50, 4 × LD50 and 8 × LD50 of strains. In all but one instance (ARM 1-4 O7:K–) it was not possible to correlate protective capacity with binding affinity of a MAb to a given bacterial cell. Therefore, the epitopes recognised by the MAb may be more closely associated with bacterial virulence than in binding to the bacterial cell.

  • Received:
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© 1997 The Pathological Society of Great Britain and Ireland
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1997-02-01
2024-04-19
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Characterisation and protective capacity of monoclonal antibodies elicited in mice against protein epitopes of antibiotic-exposed Escherichia coli
J. Med. Microbiol. 46, 122 (1997); https://doi.org/10.1099/00222615-46-2-122
/content/journal/jmm/10.1099/00222615-46-2-122
/content/journal/jmm/10.1099/00222615-46-2-122
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