1887

Abstract

Thirteen restriction endonuclease-containing strains were isolated from a collection of 186 clinical isolates of spp. Among these, eight and five isolates carried isoschizomers of RII and I, respectively. The former restriction-modification (R-M) system was homologous to that of RII and was located on plasmids with sizes of 46.6 or 55.6 kb. Isolates producing I isoschizomers contained a 5.7-kb non-transferable plasmid. Together with antimicrobial susceptibility tests and plasmid profile studies, it is concluded that these two R-M systems are not widely spread but confined to strains with similar antibiotic resistance and plasmid profile.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-46-11-949
1997-11-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/46/11/medmicro-46-11-949.html?itemId=/content/journal/jmm/10.1099/00222615-46-11-949&mimeType=html&fmt=ahah

References

  1. Roberts R. J., Macelis D. REBASE - restriction enzymes and methylases. Nucleic Acids Res 1996; 24:223–235
    [Google Scholar]
  2. Roberts R. J., Halford S. E. Type II restriction endonucleases. In Linn S. M., Lloyd R. S., Roberts R. J. (eds) Nucleases 2nd edn Cold Spring Harbor: NY, Cold Spring Harbor Press; 199335–88
    [Google Scholar]
  3. Yoshida Y., Mise K. Occurrence of small Hsd plasmids in Salmonella typhi, Shigella boydii, and Escherichia coli. J Bacteriol 1986; 165:357–362
    [Google Scholar]
  4. Mise K., Nakajima K., Terakado N., Ishidate M. Production of restriction endonucleases using multicopy Hsd plasmids occurring naturally in pathogenic Escherichia coli and Shigella boydii. Gene 1986; 44:165–169
    [Google Scholar]
  5. Naito T., Kusano K., Kobayashi I. Selfish behavior of restriction-modification systems. Science 1995; 267:897–899
    [Google Scholar]
  6. Hong Kong Government Department of Health. Notification of Infectious Diseases1990–92
    [Google Scholar]
  7. Ling J., Kam K. M., Lam A. W., French G. L. Susceptibilities of Hong Kong isolates of multiply resistant Shigella spp. to 25 antimicrobial agents, including ampicillin plus sulbactam and new 4-quinolones. Antimicrvb Agents Chemother 1988; 32:20–23
    [Google Scholar]
  8. Ling J. M., Shaw P. C., Kam K. M., Cheng A. F., French G. L. Molecular studies of plasmids of multiply-resistant Shigella spp. in Hong Kong. Epidemiol Infect 1993; 110:437–446
    [Google Scholar]
  9. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol 1976; 127:1529–1537
    [Google Scholar]
  10. Leung S. M., Kam K. M., Chan K. Y., Shaw P. C. Purification and characterization of restriction endonuclease Bco I from a soil isolate of Bacillus coagulans. FEMS Microbiol Lett 1990; 66:153–156
    [Google Scholar]
  11. Kado C. L., Liu S.-T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145:1365–1373
    [Google Scholar]
  12. Sambrook J., Fritsch E. F., Maniatis T. Molecular cloning: a laboratory manual. 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989
    [Google Scholar]
  13. Wilson G. G. Organization of restriction-modification systems. Nucleic Acids Res 1991; 19:2539–2566
    [Google Scholar]
  14. Miyahara M., Maruyama T., Wake A., Mise K. Widespread occurrence of the restriction endonuclease Yenl, an isoschizomer of Pstl, in Yersinia entercolitica serotype 08. Appl Environ Microbiol 1988; 54:577–580
    [Google Scholar]
  15. Miyahara M., Kudoh Y., Mise K. Widespread occurrence of specific restriction endonucleases in Salmonella infantis, Salmonella thompson, and Salmonella blockley isolated from humans in Japan. Appl Environ Microbiol 1990; 56:2248–2250
    [Google Scholar]
  16. Rouland-Doussoix D., Yoshimori R., Greene P., Betlach M., Goodman H. M., Boyer H. W. R factor-controlled restriction and modification of deoxyribonucleic acid. In Schlessinger D. (ed) Microbiology Washington, DC: American Society for Microbiology; 1975187–198
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-46-11-949
Loading
/content/journal/jmm/10.1099/00222615-46-11-949
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error