1887

Abstract

A rapid, simplified procedure combining random amplified polymorphic DNA (RAPD) fingerprinting of boiled cultures with high resolution agarose gel electrophoresis was used to compare strains from 46 pathogenic leptospiral serovars. The serovars were placed in eight groups on the basis of RAPD profile similarities. Groups 1–7 corresponded with the genome species and . The eighth group did not correspond with a known genome species and may represent a new genome species. Primer choice determined the degree of discrimination possible between closely related serovars and genotypes. This procedure, unlike other procedures used for analysing taxonomic relationships between leptospiral serovars, does not require extensive DNA purification, polyacrylamide gel electrophoresis or autoradiography.927

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1997-11-01
2022-01-20
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References

  1. Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990; 18:6531–6535
    [Google Scholar]
  2. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990; 18:7213–7218
    [Google Scholar]
  3. Brousseau R., Saint-Onge A., Préfontaine G., Masson L., Cabana J. Arbitrary primer polymerase chain reaction, a powerful method to identify Bacillus thuringiensis serovars and strains. Appl Environ Microbiol 1993; 59:114–119
    [Google Scholar]
  4. Jayarao B. M., Bassam B. J., Caetano-Anolles G., Gresshoff P. M., Oliver S. P. Subtyping of Streptococcus uberis by DNA amplification fingerprinting. J Clin Microbiol 1992; 30:1347–1350
    [Google Scholar]
  5. Mazurier S., van de Giessen A., Heuvelman K., Wernars K. RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA. Lett Appl Microbiol 1992; 14:260–262
    [Google Scholar]
  6. Welsh J., Pretzman C., Postic D., Girons I. S., Baranton G., McClelland M. Genomic fingerprinting by arbitrarily primed polymerase chain reaction resolves Borrelia burgdorferi into three distinct phyletic groups. Int J Syst Bacteriol 1992; 42:370–377
    [Google Scholar]
  7. Ralph D., McClelland M., Welsh J., Baranton G., Perolat P. Leptospira species categorized by arbitrarily primed polymerase chain reaction (PCR) and by mapped restriction polymorphisms in PCR-amplified rRNA genes. J Bacteriol 1993; 175:973–981
    [Google Scholar]
  8. Corney B. G., Colley J., Djordjevic S. P., Whittington R., Graham G. C. Rapid identification of some Leptospira isolates from cattle by random amplified polymorphic DNA fingerprinting. J Clin Microbiol 1993; 31:2927–2932
    [Google Scholar]
  9. Pérolat P., Merien F., Ellis W. A., Baranton G. Characterization of Leptospira isolates ffom serovar hardjo by ribotyping, arbitrarily primed PCR, and mapped restriction site polymorphisms. J Clin Microbiol 1994; 32:1949–1957
    [Google Scholar]
  10. Ramadass P., Jarvis B. D. W., Comer R. J., Penny D., Marshall R. B. Genetic characterization of pathogenic Leptospira species by DNA hybridization. Int J Syst Bacteriol 1992; 42:215–219
    [Google Scholar]
  11. Yasuda P. H., Steigerwalt A. G., Sulzer K. R., Kauffmann A. F., Rogers F., Brenner D. J. Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospimceae with proposals for seven new Leptospira species. Int J Syst Bacteriol 1987; 37:407–415
    [Google Scholar]
  12. Ramadass P., Jarvis B. D. W., Comer R. J., Cinco M., Marshall R. B. DNA relatedness among strains of Leptospira biflexa. Int J Syst Bacteriol 1990; 40:231–235
    [Google Scholar]
  13. Sambrook J., Fritsch E. F., Maniatis T. Molecular cloning: alaboratory manual. 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989
    [Google Scholar]
  14. Faine S. Leptospira and leptospirosis. Boca Raton, FL: CRC Press; 1994
    [Google Scholar]
  15. Thiermann A. B., Handsaker A. L., Foley J. W., White F. H., King-scote B. F. Reclassification of North American leptospiral isolates belonging to serogroups Mini and Sejroe by restriction endonuclease analysis. Am J Vet Res 1986; 47:61–66
    [Google Scholar]
  16. Pérolat P., Lecuyer I., Postic D., Baranton G. Diversity of ribosomal DNA fingerprints of Leptospira serovars provides a database for subtyping and species assignation. Res Microbiol 1993; 144:5–15
    [Google Scholar]
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