1887

Abstract

has emerged in the last few years as an important nosocomial pathogen. Many methods for typing this organism have been described. In this study the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was shown to be a convenient typing method for . Different combinations of primers previously used for typing other gram-negative bacilli were assessed. The combination of primer HLWL-74 and 1254 gave distinguishable patterns for different serotypes and proved to be the most satisfactory. By applying this combination to 175 isolates of , which could be classified into 38 groups on the basis of serotyping and phage typing, 73 different RAPD patterns with good reproducibility were obtained. This is, to our knowledge, the first application of the method to a large collection of S. rnarcescens representing a wide range of serotypes.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-46-11-913
1997-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/46/11/medmicro-46-11-913.html?itemId=/content/journal/jmm/10.1099/00222615-46-11-913&mimeType=html&fmt=ahah

References

  1. Cox C. E. Aztreonam therapy for complicated urinary tract infections caused by multidrug-resistant bacteria. Rev Infect Dis 1985; 7: Suppl 1S767–S770
    [Google Scholar]
  2. Sautter R. L., Mattman H. L., Legaspi R. C. Serratia marcescens meningitis associated with contaminated benzalkonium chloride solution. Infect Control 1984; 5:223–225
    [Google Scholar]
  3. Körner R. J., Nicol A., Reeves D. S., MacGowan A. P., Hows J. Ciprofloxacin resistant Serratia marcescens endocarditis as a complication of non-Hodgkin’s lymphoma. J Infect 1994; 29:73–76
    [Google Scholar]
  4. Heltberg O., Skov F., Gerner-Smidt P. Nosocomial epidemic of Serratia marcescens septicemia ascribed to contaminated blood transfusion bags. Transfusion 1993; 33:221–227
    [Google Scholar]
  5. Hogman C. F., Fritz H., Sandberg L. Posttransfusion Serratia marcescens septicemia (editorial). Transfusion 1993; 33:189–191
    [Google Scholar]
  6. Coria-Jimenes R., Ortiz-Torres C. Aminoglycoside resistance patterns of Serratia marcescens strains of clinical origin. Epidemiol Infect 1994; 112:125–131
    [Google Scholar]
  7. Wheat R. P., Zuckerman A., Rantz L. A. Infection due to chromobacteria: report of eleven cases. AMA Arch Intern Med 1951; 88:461–466
    [Google Scholar]
  8. Larose P., Picard B., Thibault M., Grimont F., Goullet P. Nosocomial Serratia marcescens individualized by five typing methods in a regional hospital. J Hosp Infect 1990; 15:167–172
    [Google Scholar]
  9. Rubin S. J. Biotyping, serotyping, zymotyping and antibiogram typing of Serratia marcescens. In The genus Serratia Florida: CRC Press; 1980101–118
    [Google Scholar]
  10. Pitt T. L. State of the art: typing of Serratia marcescens. J Hosp Infect 1982; 3:9–14
    [Google Scholar]
  11. Chetoui H., Delhalle E., Osterrieth P., Rousseaux D. Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping. J Clin Microbiol 1995; 33:2637–2642
    [Google Scholar]
  12. Bingen E. H., Mariani-Kurkdjian P., Lambert-Zechovsky N. Y. Ribotyping provides efficient differentiation of nosocomial Serratia marcescens isolates in a paediatric hospital. J Clin Microbiol 1992; 30:2088–2091
    [Google Scholar]
  13. Pitt T. L., Erdman Y. J. Serological typing of Serratia marcescens. Methods Microbiol 1984; 15:173–211
    [Google Scholar]
  14. Aucken H. M., Merkouroglou M., Miller A. W., Galbraith L., Wilkinson S. G. Structural and serological studies of lipopoly-saccharides from proposed new serotypes (025 and 026) of Serratia marcescens. FEMS Microbiol Lett 1995; 130:267–272
    [Google Scholar]
  15. Pitt T. L., Erdman Y. J., Bucher C. The epidemiological type identification of Serratia marcescens from outbreaks of infection in hospitals. J Hyg 1980; 84:269–283
    [Google Scholar]
  16. Alonso R., Aucken H. M., Perez-Diaz J. C., Cookson B. D., Baquero F., Pitt T. L. Comparison of serotype, biotype and bacteriocin type with rDNA RFLP patterns for the type identification of Serratia marcescens. Epidemiol Infect 1993; 111:99–107
    [Google Scholar]
  17. Arzese A., Botta G. A., Gesu G. P., Schito P. Evaluation of a computer-assisted method of analysing SDS-PAGE protein profiles in tracing a hospital outbreak of Serratia marcescens. J Infect 1988; 17:35–42
    [Google Scholar]
  18. Duggan T. G., Leng R. A., Hancock B. M., Cursons R. T. Serratia marcescens in a newborn unit - microbiological features. Pathology 1984; 16:189–191
    [Google Scholar]
  19. Miranda G., Kelly C., Solorzano F., Leanos B., Coria R., Patterson J. E. Use of pulsed-field gel electrophoresis typing to study an outbreak of infection due to Serratia marcescens in a neonatal intensive care unit. J Clin Microbiol 1996; 34:3138–3141
    [Google Scholar]
  20. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990; 18:7213–7218
    [Google Scholar]
  21. Xia H. X., Windle H. J., Marshall D. G., Smyth C. J., Keane C. T., O’Morain C. A. Recrudescence of Helicobacter pylori after apparently successful eradication: novel application of randomly amplified polymorphic DNA fingerprinting. Gut 1995; 37:30–34
    [Google Scholar]
  22. Marshall D. G., Chua A., Keeling P. W. N., Sullivan D. J., Coleman D. C., Smyth C. J. Molecular analysis of Helicobacter pylori populations in antral biopsies from individual patients using randomly amplified polymorphic DNA (RAPD) fingerprinting. FEMS Immunol Med Microbiol 1995; 10:317–323
    [Google Scholar]
  23. Bingen E., Biossinot C., Desjardins P. Arbitrarily primed polymerase chain reaction provides rapid differentiation of Proteus mirabilis isolates from a pediatric hospital. J Clin Microbiol 1993; 31:1055–1059
    [Google Scholar]
  24. Madico G., Akopyants N. S., Berg D. E. Arbitrarily primed PCR DNA fingerprinting of Escherichia coli O157: H7 strains by using templates from boiled cultures. J Clin Microbiol 1995; 33:1534–1536
    [Google Scholar]
  25. Grattard F., Pozzetto B., Berthelot P. Arbitrarily primed PCR, ribotyping, and plasmid pattern analysis applied to investigation of a nosocomial outbreak due to Enterobacter cloacae in a neonatal intensive care unit. J Clin Microbiol 1994; 32:596–602
    [Google Scholar]
  26. Bosi C., Davin-Regli A., Charrel R., Rocca B., Monnet D., Bollet C. Serratia marcescens nosocomial outbreak due to contamination of hexetidine solution. J Hosp Infect 1996; 33:217–224
    [Google Scholar]
  27. Coleman D., Falkiner F. R., Carr M. E., Dowd G., Dougan G., Keane C. T. Simultaneous oubreaks of infection due to Serratia marcescens in a general hospital. J Hosp Infect 1984; 5:270–282
    [Google Scholar]
  28. Grimont P. A. D., Grimont F. Biotyping of Serratia marcescens and its use for epidemiological studies. J Clin Microbiol 1978; 8:73–83
    [Google Scholar]
  29. Liu P. Y.-F., Lau Y.-J., Hu B.-S. Use of PCR to study epidemiology of Serratia marcescens isolates in nosocomial infection. J Clin Microbiol 1994; 32:1935–1938
    [Google Scholar]
  30. Akopyanz N., Bukanov N. O., Westblom T. U., Kresovich S., Berg D. E. DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res 1992; 20:5137–5142
    [Google Scholar]
  31. Riain U. N., Cormican M. G., Flynn J., Smith T., Glennon M. PCR based fingerprinting of Enterobacter cloacae. J Hosp Infect 1994; 27:237–240
    [Google Scholar]
  32. Power E. G. M. RAPD typing in microbiology - a technical review. J Hosp Infect 1996; 34:247–265
    [Google Scholar]
  33. Innis M. A., Gelfand D. H. Optimization of PCRs. In Innis M. A., Gelfand D. H., Sninsky J. J., White T. L. (eds) PCR protocols. A guide to methods and applications San Diego: Academic Press; 19903–20
    [Google Scholar]
  34. Cookson B. D., Aparicio P., Deplano A., Struelens M., Goering R., Marples R. Inter-centre comparison of pulsed-field gel electrophoresis for the typing of methicillin-resistant Staphylococcus aureus. J Med Microbiol 1996; 44:179–184
    [Google Scholar]
  35. Tenover F. C., Arbeit R. D., Goering R. V. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33:2233–2239
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-46-11-913
Loading
/content/journal/jmm/10.1099/00222615-46-11-913
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error