Mucosa-associated bacterial flora of the human colon Free

Abstract

Biopsy samples of mucosa were taken during colonoscopy from the proximal colon and rectum of 12 patients, six with ulcerative colitis (UC) and six with non-inflammatory conditions. After anaerobic transport to the laboratory, biopsy specimens were examined by quantitative bacteriological culture on selective and non-selective media for total aerobic count, total anaerobic count, spp., lactobacilli, bifidobacteria and asaccharolytic, lactic acid producers. Isolates of the genus were identified to species level. Counts from proximal colonic and rectal biopsy samples in the same patient were not significantly different. Viable aerobic counts (aerobes and facultative organisms) ranged from 2.4 × 10 to 1.3 × 10 cfu/sample biopsy (5.6 mg) and total anaerobic counts were 10–10 times higher at (1.4 × 10)–(3 × 10) cfu/sample. spp. predominated at both sites (range 8.6 × 10 to 1.4 × 10 cfu/sample), comprising 66% of total counts from proximal colon (range in individual patients 31–80%) and 68.5% from rectum (range 38–91%). Lactobacilli were isolated from eight biopsy samples from five patients, counts ranging from 3.6 × 10 to 1 × 10 cfu/sample; bifidobacteria were isolated from both sites from 10 of the 12 patients, counts ranging from 50 to 1.8 × 10 cfu/sample. From the 24 biopsy samples, 235 isolates representing 11 species of were identified. For any individual patient, only a few species (2–7; mean 4.4) of were found, with just one or two species predominating. was cultured from both samples of seven patients (where it was the major isolate in four) and from single samples of two others; was cultured from both sites in six patients, being the major isolate in one patient and second commonest in three, but was not detected in the other six; the majority of other isolates were and was isolated from both biopsy samples in all three UC patients with active inflammation (16 of the 60 isolates from these patients) but from only four of the other 18 samples from non-inflamed colonic mucosa (nine of 175 isolates).

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1997-01-01
2024-03-28
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References

  1. Duerden B. I., Drasar B. S. Anaerobes in human disease. London: Edward Arnold; 1991
    [Google Scholar]
  2. Namavar F., Theunissen E. B. M., Verweij-Van Vught A. M. J. J., et al. Epidemiology of the Bacteroides fragilis group in the colonic flora in 10 patients with colonic cancer. J Med Microbiol 1989; 29:171–176
    [Google Scholar]
  3. Peach S. L., Tabaqchali S. Mucosa-associated flora of the human gastrointestinal tract in health and disease. Eur J Chemother Antibiot 1982; 2:41–50
    [Google Scholar]
  4. Hudson M. J., Hill M. J., Elliott P. R., Berghouse L. M., Bumham W. R, Lennard-Jones J. E. The microbial flora of the rectal mucosa and faeces of patients with Crohn’s disease before and during antimicrobial chemotherapy. J Med Microbiol 1984; 18:335–345
    [Google Scholar]
  5. Hudson M. J. Anaerobes and inflammatory bowel disease. In Borriello S. P., Hardie J. M. (eds) Recent advances in anaerobic bacteriology Dordrecht: Nijhoff; 198760–71
    [Google Scholar]
  6. Hartley M. G., Hudson M. J., Swarbrick E. T., Grace R. H., Gent A. E., Hellier M. D. Sulphasalazine treatment and the colorectal mucosa-associated flora in ulcerative colitis. Aliment Pharmacol Ther 1996; 10:157–163
    [Google Scholar]
  7. McCourtie J., Poxton I. R., Spence J. A., Aitchison G. U. Preliminary study of the anaerobic bacteria isolated from subgingival plaque from sheep. Vet Microbiol 1989; 21:139–146
    [Google Scholar]
  8. Brown R., Collee J. G., Poxton I. R. Bacteroides, Fusobacterium and other Gram negative rods; anaerobic cocci; identification of anaerobes. In Collee J. G., Fraser A. G., Marmion B. P., Simmons A. (eds) Mackie and McCartney’s Practical medical microbiology 14th edn New York; Churchill Livingstone: 1996501–519
    [Google Scholar]
  9. Giaffer M. H., Holdsworth C. D., Duerden B. L. The assessment of faecal flora in patients with inflammatory bowel disease by a simplified bacteriological technique. J Med Microbiol 1991; 35:238–243
    [Google Scholar]
  10. Brook I. Recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals. J Clin Microbiol 1988; 26:1181–1188
    [Google Scholar]
  11. Brook I. Bacteroides infections in children. J Med Microbiol 1995; 43:92–98
    [Google Scholar]
  12. Onderdonk A. B., Kasper D. L., Cisneros R. L., Bartlett J. G. The capsular polysaccharide of Bacteroides fragilis as a virulence factor: comparison of the pathogenic potential of encapsulated and unencapsulated strains. J Infect Dis 1977; 136:82–89
    [Google Scholar]
  13. Casciato D. A., Rosenblatt J. E., Bluestone R., Goldberg L. S., Finegold S. M. Susceptibility of isolates of Bacteroides to the bactericidal activity of normal human serum. J Infect Dis 1979; 140:109–113
    [Google Scholar]
  14. Rotimi V. O., Eke P. L. The bactericidal action of human serum on Bacteroides species. J Med Microbiol 1984; 18:355–363
    [Google Scholar]
  15. Allan E., Poxton I. R. The influence of growth medium on serum sensitivity of Bacteroides species. J Med Microbiol 1994; 41:45–50
    [Google Scholar]
  16. Botta G. A., Arzese A., Minisini R., Trani G. Role of structural and extracellular virulence factors in gram-negative anaerobic bacteria. Clin Infect Dis 1994; 18: Suppl 4S260–S264
    [Google Scholar]
  17. Brook I. The role of encapsulated anaerobic bacteria in synergistic infections. FEMS Microbiol Rev 1994; 13:65–74
    [Google Scholar]
  18. Patrick S. The virulence of Bacteroides fragilis. Rev Med Microbiol 1993; 4:40–49
    [Google Scholar]
  19. Tzianabos A. O., Onderdonk A. B., Rosner B., Cisneros R. L., Kasper D. L. Structural features of polysaccharides that induce intraabdominal abscesses. Science 1993; 262:416–419
    [Google Scholar]
  20. Tzianabos A. O., Onderdonk A. B., Smith R. S., Kasper D. L. Structure-function relationships for polysaccharide-induced intra-abdominal abscesses. Infect Immun 1994; 62:3590–3593
    [Google Scholar]
  21. Poxton I. R., Edmond D. M. Biological activity of Bacteroides lipopolysaccharide - reappraisal. Clin Infect Dis 1995; 20: Suppl 2S149–S153
    [Google Scholar]
  22. Delahooke D. M., Barclay G. R., Poxton I. R. A re-appraisal of the biological activity of bacteroides lipopolysaccharide. J Med Microbiol 1995; 42:102–112
    [Google Scholar]
  23. Delahooke D. M., Barclay G. R., Poxton I. R. Tumor necrosis factor induction by an aqueous phenol-extracted lipopolysaccharide complex from Bacteroides species. Infect Immun 1995; 63:840–846
    [Google Scholar]
  24. Allan E., Poxton I. R., Barclay G. R. Anti-bacteroides lipopolysaccharide IgG levels in healthy adults and sepsis patients. FEMS Immunol Med Microbiol 1995; 11:5–12
    [Google Scholar]
  25. Poxton I. R., Myers C. J., Johnstone A., Drudy T. A., Ferguson A. An ELISA to measure mucosal IgA specific for Bacteroides surface antigens in whole gut lavage fluid. Microbial Ecol Hlth Dis 1995; 8:129–136
    [Google Scholar]
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