1887

Abstract

In April 1994, was isolated from five infants with watery diarrhoea in Arequipa, Peru, as part of a passive cholera surveillance system. The children ranged in age from 11 to 20 months and had acute diarrhoea, with two cases showing moderate dehydration. Two children also had traces of blood in liquid stool. The children were seen at two different hospitals, and no evidence of a common source of infection was found. No additional isolates were identified in the remaining surveillance period that covered the rest of 1994 and 1995. However, stool samples were not screened for enteric pathogens other than vibrios. strains isolated from stool samples produced opaque and translucent colonies on agar plates, suggesting capsular material. All isolates were resistant to ampicillin, erythromycin and streptomycin. Plasmid analysis revealed a common 200-kb plasmid in isolates from all cases and an additional 2.7-kb plasmid in three of the isolates. Ribotyping of each isolate after restriction with /I and III endonucleases demonstrated identical ribotyping patterns. The cases reported suggest that may be associated with diarrhoea in man by mechanisms so far unknown.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-45-6-494
1996-12-01
2024-11-08
Loading full text...

Full text loading...

/deliver/fulltext/jmm/45/6/medmicro-45-6-494.html?itemId=/content/journal/jmm/10.1099/00222615-45-6-494&mimeType=html&fmt=ahah

References

  1. Kelly M. T., Hickman-Brenner F. W., Farmer J. J. Vibrio. In Balows A., Hausler W. J., Herrmann K. L., Isenberg H. D., Shadomy H. J. (eds) Manual of clinical microbiology 5th edn Washington, DC: American Society for Microbiology; 1991384–395
    [Google Scholar]
  2. Gamaleia M. N. Vibrio metschnikovii (n. sp.) et ses rapports avec le microbe du cholera asiatique. Ann Inst Pasteur (Paris) 1888; 2:482–488
    [Google Scholar]
  3. Lee J. V., Donovan T. J., Fumiss A. L. Characterization, taxonomy, and emended description of Vibrio metschnikovii . Int J Syst Bacteriol 1978; 28:99–111
    [Google Scholar]
  4. Farmer J. J., Hickman-Brenner F. W., Fanning G. R., Gordon C. M., Brenner D. J. Characterization of Vibrio metschnikovii and Vibrio gazogenes by DNA-DNA hybridization and phenotype. J Clin Microbiol 1988; 26:1993–2000
    [Google Scholar]
  5. Jean-Jacques W., Rajashekaraiah K. R., Farmer J. J., Hickman F. W., Morris J. G., Kallick C. A. Vibrio metschnikovii bacteremia in a patient with cholecystitis. J Clin Microbiol 1981; 14:711–712
    [Google Scholar]
  6. Hansen W., Freney J., Benyagoub H., Letouzey, M-N., Gigi J., Wauters G. Severe human infections caused by Vibrio metschnikovii . J Clin Microbiol 1993; 31:2529–2530
    [Google Scholar]
  7. Miyake M., Honda T., Miwatani T. Purification and characterization of Vibrio metschnikovii cytolysin. Infect Immun 1988; 56:954–960
    [Google Scholar]
  8. Popovic T., Bopp C. A., Olsvik, Ø., Wachsmuth K. Epidemiologic application of a standardized ribotype scheme for Vibrio cholerae 01. J Clin Microbiol 1993; 31:2474–2482
    [Google Scholar]
  9. Dalsgaard A., Serichantalergs O., Pitarangsi C., Echeverria P. Molecular characterization and antibiotic susceptibility patterns of Vibrio cholerae non-Ol. Epidemiol Infect 1995; 114:51–63
    [Google Scholar]
  10. Dalsgaard A., Echeverria P., Larsen J. L., Siebeling R., Serichantalergs O., Huss H. H. Application of ribotyping for differentiating Vibrio cholerae non-Ol isolates from shrimp farms in Thailand. Appl Environ Microbiol 1995; 61:245–251
    [Google Scholar]
  11. Sakazaki R. Bacteriology of vibrio and related organisms. In Barua D., Greenough W. B. (eds) Cholera New York: Plenum Medical Book Company; 199237–55
    [Google Scholar]
  12. Ericsson H. M., Sherris J. C. Antibiotic sensitivity testing. Report of an international collaborative study. Acta Path Microbiol Scand B Microbiol Immunol 1971; 217: Suppl
    [Google Scholar]
  13. Kado C. l., Liu, S-T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145:1365–1373
    [Google Scholar]
  14. Olsen J. E., Larsen J. L. Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid. Appl Environ Microbiol 1990; 56:3130–3132
    [Google Scholar]
  15. Rochelle P. A., Fry J. C., Day M. J., Bale M. J. An accurate method for estimating sizes of small and large plasmids and DNA fragments by gel electrophoresis. J Gen Microbiol 1985; 132:53–59
    [Google Scholar]
  16. Threlfall E. J., Rowem B., Ferguson J. L., Ward L. R. Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain. J Hyg 1986; 97:419–426
    [Google Scholar]
  17. Murray M. G., Thompson W. F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 1980; 8:4321–4325
    [Google Scholar]
  18. Olsen J. E., Larsen J. L. Ribotypes and plasmid contents of Vibrio anguillarum strains in relation to serovar. Appl Environ Microbiol 1993; 59:3863–3870
    [Google Scholar]
  19. Kreger A., DeChatelet L., Shirley P. Interaction of Vibrio vulnificus with human polymorphonuclear leukocytes: association of virulence with resistance to phagocytosis. J Infect Dis 1981; 144:244–248
    [Google Scholar]
  20. Simpson L. M., White V. K., Zane S. F., Oliver J. D. Correlation between virulence and colony morphology in Vibrio vulnificus . Infect Immun 1987; 55:269–272
    [Google Scholar]
  21. Zakaria-Meehan Z., Massad G., Simpson L. M., Travis J. C., Oliver J. D. Ability of Vibrio vulnzjicus to obtain iron from hemoglobin-haptoglobin complexes. Infect Immun 1988; 56:275–277
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-45-6-494
Loading
/content/journal/jmm/10.1099/00222615-45-6-494
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error