1887

Journal of Medical Microbiology logo

Volume 45, Issue 5

Serotyping and categorisation of strains isolated between 1958 and 1992 from diarrhoeal diseases in Asia Free

Abstract

A total of 3065 strains of isolated between 1958 and 1992 from patients with diarrhoea in different countries were examined for virulence factors by hybridisation with biotinylated DNA probes for genes that coded for production of heat-labile and heat-stable enterotoxins, enteroinvasiveness, production of verotoxins and attaching-and-effacing factor and were serotyped. Of the 3065 strains, 1998 were placed into one of four pathogenic categories by their virulence factors: 1057 enterotoxigenic (ETEC) comprising 30 O-groups, 73 serovars and 137 untypable strains; 132 enteroinvasive (EIEC) comprising 11 O-groups and 13 serovars; 64 verotoxin-producing (VTEC) comprising 11 O-groups, 17 serovars and 13 untypable strains; and 745 enteropathogenic (EPEC) comprising 34 O-groups, 92 serovars and 91 untypable strains. The remaining 1067 strains did not hybridise with any of the DNA probes used. About half the number of O-groups recognised were not restricted to a single pathogenic category, although the combinations of O- and H-antigens were different in each category.

  • Received:
  • Revised:
  • Published Online:
© 1996 The Pathological Society of Great Britain and Ireland
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-45-5-353
1996-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/45/5/medmicro-45-5-353.html?itemId=/content/journal/jmm/10.1099/00222615-45-5-353&mimeType=html&fmt=ahah

References

  1. Sakazaki R., Tamura K., Saito M. Enteropathogenic Escherichia coli associated with diarrhoea in children and adults. Jpn J Med Sci Biol 1967; 20:387–399
     [Google Scholar]
  2. Sakazaki R., Tamura K., Nakamura A. Further studies on enteropathogenic Escherichia coli associated with diarrhoeal diseases in children and adults. Jpn J Med Sci Biol 1974; 27:7–18
     [Google Scholar]
  3. Ewing W. H. Edwards and Ewing’s Identification of Enterobac-teriaceae. 4th edn New York: Elsevier; 1986
     [Google Scholar]
  4. Echeverria R., Seriwatana J., Sethabutr O., Chatkaemorakot A. Detection of diarrhoeagenic Escherichia coli using nucleotide probes. In Macario A. T. L., Conway de Macario E. (eds) Microbial gene Orlando: Academic Press; 199095–141
     [Google Scholar]
  5. Jerse A. E., Yu J., Tall B. D., Kaper J. B. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci USA 1990; 87:7839–7843
     [Google Scholar]
  6. Gicquelais K. G., Baldini M. M., Martinez J. Practical and economical method for using biotinylated DNA probes with bacterial colony blots to identify diarrhea-causing Escherichia coli. J Clin Microbiol 1990; 28:2485–2490
     [Google Scholar]
  7. Dallas W. S., Falkow S. The molecular nature of heat-labile enterotoxin (LT) of Escherichia coli. Nature 1979; 277:406–407
     [Google Scholar]
  8. Sommerfelt H., Kalland K. G., Raj P., Moseley S. L., Bhan M. K., Bjorvatn B. Cloned polynucleotide and synthetic oligonucleotide probes used in colony hybridization are equally efficient in the identification of enterotoxigenic Escherichia coli. J Clin Microbiol 1988; 26:2275–2278
     [Google Scholar]
  9. Boileau C. R., d’Hauteville H. M., Sansonetti P. J. DNA hybridization technique to detect Shigella species and enteroinvasive Escherichia coli. J Clin Microbiol 1984; 20:959–961
     [Google Scholar]
  10. Newland J. W., Neill R. J. DNA probes for Shiga-like toxins I and II toxin-converting bacteriophages. J Clin Microbiol 1988; 26:1292–1297
     [Google Scholar]
  11. Nataro J. P., Scaletsky I. C. A., Kaper J. B., Levine M. M., Trabulsi L. R. Plasmid-mediated factors conferring diffuse and localized adherence of enteropathogenic Escherichia coli. Infect Immun 1985; 48:378–383
     [Google Scholar]
  12. Schmidt H., Knop C., Franke S., Aleksic S., Heesemann J., Karch H. Development of PCR for screening of enteroaggregative Escherichia coli. J Clin Microbiol 1995; 33:701–705
     [Google Scholar]
  13. Bhan M. K., Raj P., Levine M. M. Enteroaggregative Escherichia coli associated with persistent diarrhoea in a cohort of rural children in India. J Infect Dis 1989; 159:1061–1064
     [Google Scholar]
  14. Cravioto A., Tello A., Navarro A. Association of Escherichia coli HEp-2 adherence patterns with type and duration of diarrhoea. Lancet 1991; 337:262–264
     [Google Scholar]
  15. Scotland S. M., Smith H. R., Said B., Willshaw G. A., Cheasty T., Rowe B. Identification of enteropathogenic Escherichia coli isolated in Britain as enteroaggregative or as members of a subclass of attaching-and-effacing E. coli not hybridising with the EPEC adherence-factor probe. J Med Microbiol 1991; 35:278–283
     [Google Scholar]
  16. Giron J. A., Jones T., Millan-Velasco F. Diffuse-adhering Escherichia coli (DAEC) as a putative cause of diarrhea in Mayan children in Mexico. J Infect Dis 1991; 163:507–513
     [Google Scholar]
  17. Gomes T. A. T., Blake P. A., Trabulsi L. R. Prevalence of Escherichia coli strains with localized, diffuse, and aggregative adherence to HeLa cells in infants with diarrhea and matched controls. J Clin Microbiol 1989; 27:266–269
     [Google Scholar]
  18. Tacket C. O., Moseley S. L., Kay B., Losonsky G., Levine M. M. Challenge studies in volunteers using Escherichia coli strains with diffuse adherence to HEp-2 cells. J Infect Dis 1990; 162:550–552
     [Google Scholar]
  19. Ghosh A. R., Nair G. B., Naik T. N., Mousumi, Paul., Pal S. C., Sen D. Entero-adherent Escherichia coli is an important diarrhoeagenic agent in infants aged below 6 months in Calcutta, India. J Med Microbiol 1992; 36:264–268
     [Google Scholar]
  20. Brook M. G., Smith H. R., Bannister B. A. Prospective study of verocytotoxin-producing, enteroaggregative and diffusely adherent Escherichia coli in different diarrhoeal states. Epidemiol Infect 1994; 112:63–67
     [Google Scholar]
  21. Nataro J. P., Kaper J. B., Robins-Browne R., Prado V., Vial P., Levine M. M. Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatr Infect Dis 1987; 6:829–831
     [Google Scholar]
  22. Ørskov F., Ørskov I. Escherichia coli serotyping and disease in man and animals. Can J Microbiol 1992; 38:699–704
     [Google Scholar]
  23. DeBoy J. M., I. I., Wachsmuth I. K., Davis B. R. Serotypes of enterotoxigenic Escherichia coli isolated in the United States. Infect Immun 1980; 29:361–368
     [Google Scholar]
  24. Echeverria P., Ørskov F., Ørskov I., Plianbangchang D. Serotypes of enterotoxigenic Escherichia coli in Thailand and the Philippines. Infect Immun 1982; 36:851–856
     [Google Scholar]
  25. Echeverria P., Taylor D. N., Betthelheim K. A. HeLa cell adherent Escherichia coli in children under 1 year of age in Thailand. J Clin Microbiol 1987; 25:1472–1475
     [Google Scholar]
  26. Echeverria P., Taylor D. N., Seriwatana J., Brown J. E., Lexomboom U. Examination of colonies and stool blots for detection of enteropathogens by DNA hybridization with eight DNA probes. J Clin Microbiol 1989; 27:331–334
     [Google Scholar]
  27. Echeverria P., Ørskov F., Ørskov I. Attaching and effacing enteropathogenic Escherichia coli as a cause of infantile diarrhea in Bangkok. J Infect Dis 1991; 164:550–554
     [Google Scholar]
  28. Rowe B., Gross R., Takeda Y. Serotyping and enterotoxigenic Escherichia coli isolated from diarrhoeal travellers from various countries. FEMS Microbiol Lett 1983; 20:187–189
     [Google Scholar]
  29. Bopp C. A., Greene K. D., Downes E. P., Sowers E. G., Wells J. G., Wachsmuth I. K. Unusual verotoxin-producing Escherichia coli associated with hemorrhagic colitis. J Clin Microbiol 1987; 25:1486–1489
     [Google Scholar]
  30. Betthelheim K. A., Brown J. W., Lolokha S., Echeverria P. Serotypes of Escherichia coli that hybridize with DNA probes for genes encoding Shiga-like toxin I, Shiga-like toxin II, and serogroup O157 enterohemorrhagic E. coli fimbriae from adults with diarrhea in Thiland. J Clin Microbiol 1990; 28:295–298
     [Google Scholar]
  31. Blanco J., Blanco M., Garabal J. I., Gonzalez E. A. Enterotoxins, colonization factors and serotypes of enterotoxigenic Escherichia coli from human and animals. Microbiologia 1991; 7:57–73
     [Google Scholar]
  32. Blanco J., Gonzalez E. A., Blanco M. Enterotoxigenic Escherichia coli associated with infant diarrhoea in Galicia, north-western Spain. J Med Microbiol 1991; 35:162–167
     [Google Scholar]
  33. Knutton S., Phillips A. D., Smith H. R. Screening of enteropathogenic Escherichia coli in infants with diarrhea by the fluorescent-actin staining test. Infect Immun 1991; 59:365–371
     [Google Scholar]
  34. Scotland S. M., Willshaw G. A., Smith H. R., Said B., Stokes N., Rowe B. Virulence properties of Escherichia coli strains belonging to serogroups O25, O55, O111 and O128 isolated in the United Kingdom in 1991 from patients with diarrhoea. Epidemiol Infect 1993; 111:429–438
     [Google Scholar]
  35. Bockemuhl J., Aleksic S., Karch H. Serological and biochemical properties of Shiga-like toxin (verocytotoxin)-producing strains of Escherichia coli, other than O-group 157, from patients in Germany. Int J Med Microbiol Virol Parasitol Infect Dis 1992; 276:189–195
     [Google Scholar]
  36. Wolf M. K., Taylor D. N., Boedeker E. C. Characterization of enterotoxigenic Escherichia coli isolated from U.S. troops deployed to the Middle East. J Clin Microbiol 1993; 31:851–856
     [Google Scholar]
  37. Law D. Adhesion and its role in the virulence of enteropathogenic Escherichia coli. Clin Microbiol Rev 1994; 7:152–173
     [Google Scholar]
  38. Campos L. C., Whittman T. S., Gomez T. A. T., Andrade J. R. C., Trabulsi L. R. Escherichia coli serogroup O111 includes several clones of diarrheagenic strains with different virulence properties. Infect Immun 1994; 62:3282–3288
     [Google Scholar]
  39. Yam W. C., Robins-Browne R. M., Lung M. L. Genetic relationships and virulence factors among classical enteropathogenic Escherichia coli serogroup 0126 strains. J Med Microbiol 1994; 40:229–235
     [Google Scholar]
  40. Schmidt H., Rüssmann H., Karch H. Virulence determinants in nontoxinogenic Escherichia coli 0157 strains that cause infantile diarrhea. Infect Immun 1993; 61:4894–4898
     [Google Scholar]
  41. Scotland S. M., Willshaw G. A., Cheasty T., Rowe B. Strains of Escherichia coli O157:H8 from human diarrhoea belong to attaching and effacing class of E. coli. J Clin Pathol 1992; 45:1075–1078
     [Google Scholar]
  42. Zadik P. M., Chapman P. A., Siddons C. A. Use of tellurite for the selection of verocytotoxigenic Escherichia coli O157. J Med Microbiol 1993; 39:155–158
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-45-5-353
Loading
Serotyping and categorisation of Escherichia coli strains isolated between 1958 and 1992 from diarrhoeal diseases in Asia
J. Med. Microbiol. 45, 353 (1996); https://doi.org/10.1099/00222615-45-5-353
/content/journal/jmm/10.1099/00222615-45-5-353
/content/journal/jmm/10.1099/00222615-45-5-353
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error