1887

Abstract

Pyrolysis mass spectrometry (PyMS), a highly discriminatory method for comparison of isolates, was used to assess the homogeneity of colonies taken from apparently pure cultures of in 10 urine specimens. Ten randomly selected colonies were subcultured from each urine for comparison. For six urines, the set of 10 single-colony isolates proved indistinguishable by PyMS. However, for four urines there was clear heterogeneity. For two urines, one of the isolates was distinct, and the remaining nine indistinguishable. One urine yielded seven indistinguishable isolates, and three further individually distinct isolates. One urine yielded two clusters of indistinguishable isolates, one comprising seven and the other three isolates. Thus, significant heterogeneity (as measured by PyMS) occurs in apparently pure cultures of from urinary tract infection. The nature of this heterogeneity remains to be established, as does the significance, if any, of this phenomenon in urinary tract infection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-45-5-349
1996-11-01
2019-11-13
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-45-5-349
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error