Comparative cytotoxicity of purified Shiga-like toxin-lle on porcine and bovine aortic endothelial and human colonic adenocarcinoma cells Free

Abstract

Porcine and bovine aortic endothelial cells and human colonic adenocarcinoma cells were compared for their susceptibility to the toxic effect of purified Shiga-like toxin IIe (SLT-IIe), measured by the neutral red cytotoxicity assay. Cytotoxicity correlated with toxin binding as indicated by fluorescence activated cell sorter analysis and with the globotriosylceramide (Gb) and globotetraosylceramide (Gb) content of cells determined by high pressure liquid chromatography. One line of porcine aortic endothelial cells was 1400-fold more susceptible than the line of bovine aortic endothelial cells that was tested, but a second line of porcine aortic endothelial cells was highly refractory to SLT-IIe. Human colonic adenocarcinoma cells lacked detectable levels of Gb and were least susceptible to SLT-IIe.

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1996-11-01
2024-03-29
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References

  1. Gyles C. L. Escherichia coli cytotoxins and enterotoxins. Can J Microbiol 1992; 38:734–746
    [Google Scholar]
  2. Marques L. R. M., Peiris J. S. M., Cryz S. J., O’Brien A. D. Escherichia coli strains isolated from pigs with edema disease produce a variant of Shiga-like toxin II. FEMS Microbiol Lett 1987; 44:33–38
    [Google Scholar]
  3. Meyer T., Karch H. Genes coding for Shiga-like toxin and heat-stabile enterotoxin in porcine strains of Escherichia coli. FEMS Microbiol Lett 1989; 58:115–120
    [Google Scholar]
  4. Strockbine N. A., Marques L. R. M., Newland J. W., Smith H. W., Holmes R. K., O’Brien A. D. Two toxin-converting phages from Escherichia coli 0157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infect Immun 1986; 53:135–140
    [Google Scholar]
  5. Gyles C. L., DeGrandis S. A., MacKenzie C., Brunton J. L. Cloning and nucleotide sequence analysis of the genes determining verocytotoxin production in a porcine edema disease isolate of Escherichia coli. Microb Pathog 1988; 5:419–426
    [Google Scholar]
  6. Weinstein D. L., Jackson M. P., Samuel J. E., Holmes R. K., O’Brien A. D. Cloning and sequencing of a Shiga-like toxin type II variant from an Escherichia coli strain responsible for edema disease of swine. J Bacteriol 1988; 170:4223–1230
    [Google Scholar]
  7. Gannon V. P. J., Gyles C. L. Characteristics of the Shiga-like toxin produced by Escherichia coli associated with porcine edema disease. Vet Microbiol 1990; 24:89–100
    [Google Scholar]
  8. DeGrandis S., Law H., Brunton J., Gyles C., Lingwood C. A. Globotetraosylceramide is recognized by the pig edema disease toxin. J Biol Chem 1989; 264:12520–12525
    [Google Scholar]
  9. Keusch G. T., Jacewicz M., Acheson D. W. K., Donohue-Rolfe A., Kane A. V., McCluer R. H. Globotriaosylceramide, Gb3, is an alternative functional receptor for Shiga-like toxin 2e. Infect Immun 1995; 63:1138–1141
    [Google Scholar]
  10. Head S. C., Karmali M. A., Lingwood C. A. Preparation of VT1 and VT2 hybrid toxins from their purified dissociated subunits. J Biol Chem 1991; 266:3617–3621
    [Google Scholar]
  11. Zoja C., Coma D., Farina C. Verotoxin glycoplipid receptors determine the localization of microangiopathic process in rabbits given verotoxin-1. J Lab Clin Med 1992; 120:229–238
    [Google Scholar]
  12. Boyd B., Lingwood C. Verotoxin receptor glycolipid in human renal tissue. Nephron 1989; 51:207–210
    [Google Scholar]
  13. Richardson S. E., Rotman T. A., Jay V. Experimental verocytotoxemia in rabbits. Infect Immun 1992; 60:4154–4167
    [Google Scholar]
  14. Kavi J., Chant I., Maris M., Rose P. E. Cytopathic effect of verotoxin on endothelial cells. Lancet 1987; 2:1035
    [Google Scholar]
  15. Obrig T. G., Del Vecchio P. J., Karmali M. A., Petrie M., Moran T. P., Judge T. K. Pathogenesis of haemolytic uraemic syndrome. Lancet 1987; 2:687
    [Google Scholar]
  16. Gannon V. P. J., Gyles C. L., Wilcock B. P. Effects of Escherichia coli Shiga-like toxins (verotoxins) in pigs. Can J Vet Res 1989; 53:306–312
    [Google Scholar]
  17. Moyer M. P., Dixon P. S., Rothman S. W., Brown J. E. Cytotoxicity of Shiga toxin for primary cultures of human colonic and ileal epithelial cells. Infect Immun 1987; 55:1533–1535
    [Google Scholar]
  18. MacLeod D. L., Gyles C. L., Wilcock B. P. Reproduction of edema disease of swine with purified Shiga-like toxin II variant. Vet Pathol 1991; 28:66–73
    [Google Scholar]
  19. Valdivieso-Garcia A., Rosendal S., Allen O. B., Thompson C. M., Watson S. Cytotoxicity of Mycoplasma mycoides subspecies mycoides for cultured endothelial cells. Int J Med Microbiol 1989; 272:202–209
    [Google Scholar]
  20. Gannon V. P. J., Gyles C. L., Friendship R. W. Characteristics of verotoxigenic Escherichia coli from pigs. Can J Vet Res 1988; 52:331–337
    [Google Scholar]
  21. MacLeod D. L., Gyles C. L. Purification and characterization of an Escherichia coli Shiga-like toxin II variant. Infect Immun 1990; 58:1232–1239
    [Google Scholar]
  22. MacLeod D. L., Gyles C. L. Effects of culture conditions on yield of Shiga-like toxin-IIv from Escherichia coli. Can J Microbiol 1989; 35:623–629
    [Google Scholar]
  23. Valdivieso-Garcia A., Clarke R. C., Rahn K., Durette A., MacLeod D. L., Gyles C. L. Neutral red assay for measurement of quantitative vero cell cytotoxicity. Appl Environ Microbiol 1993; 59:1981–1983
    [Google Scholar]
  24. Rovozzo G. C., Burke C. N. A manual of basic virological techniques. Englewood Cliffs, NJ: Prentice-Hall Inc; 197387–93
    [Google Scholar]
  25. Ryan U. S., White L. A., Lopez M., Ryan J. W. Use of microcarriers to isolate and culture pulmonary microvascular endothelium. Tissue Cell 1982; 14:597–606
    [Google Scholar]
  26. Tyrrell G. J., Ramotar K., Toye B., Boyd B., Lingwood C. A., Brunton J. L. Alteration of the carbohydrate binding specificity of verotoxins from Galα1-4Gal to GalNAcß1-3 Galα1-4Gal and vice versa by site-directed mutagenesis of the binding subunit. Proc Natl Acad Sci USA 1992; 89:524–528
    [Google Scholar]
  27. Grant C. W. M. Cell surface structural implications of some experiments with isolated glycolipids and glycoproteins. Can J Biochem Cell Biol 1984; 62:1151–1157
    [Google Scholar]
  28. Forstner G., Sherman P., Forstner J. Mucus: function and structure. In Boedeker E. C. (ed) Attachment of organisms to the gut mucosa vol II Boca Raton, LL: CRC Press Inc; 198414–20
    [Google Scholar]
  29. Francis D. H., Moxley D. A., Andraos C. Y. Edema disease-like brain lesions in gnotobiotic piglets infected with Escherichia coli serotype 0157:H7. Infect Immun 1989; 57:1339–1342
    [Google Scholar]
  30. Wyrick P. B., Choong J., Davis C. H Entry of genital Chlamydia trachomatis into polarized human epithelial cells. Infect Immun 1989; 57:2378–2389
    [Google Scholar]
  31. Pudymaitis A., Lingwood C. A. Susceptibility to verotoxin as a function of the cell cycle. J Cell Physiol 1992; 150:632–639
    [Google Scholar]
  32. Boyd B., Tyrrell G., Malonbey M., Gyles C., Brunton J., Lingwood C. Alteration of the glycolipid binding specificity of the pig edema toxin from globotetraosyl to globotriaosyl ceramide alters in vivo tissue targetting and results in a verotoxin 1-like disease in pigs. J Exp Med 1993; 177:1745–1753
    [Google Scholar]
  33. Orbrig T. G., Louise C. B., Lingwood C. A., Boyd B., Barley-Maloney L., Daniel T. O. Endothelial heterogeneity in Shiga toxin receptors and responses. J Biol Chem 1993; 268:15484–15488
    [Google Scholar]
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