1887

Abstract

Random amplified polymorphic DNA (RAPD) analysis was evaluated for its capacity to distinguish species and strains within species of groups A, C and G streptococci. The 99 strains tested, previously typed by multilocus enzyme electrophoresis (MLEE), included 41 group A streptococci (), 25 group G . (GGS), seven , 11 , four , three and eight . The combined data obtained with three single primers distinguished 82 types. RAPD analysis provided taxonomic results that were in general agreement with previous species classification based on DNA-DNA homology and MLEE. The intraspecies typing efficiency of the technique was significantly improved by the parallel use of several primers. RAPD analysis had greater discriminatory power than MLEE for GAS and GGS. There was not total agreement between the two techniques as RAPD distinguished strains with identical electrophoretic types, whereas MLEE differentiated strains with identical PCR types. RAPD analysis did not distinguish all GAS strains with different biotypes and its already high discriminatory power was further enhanced by concomitant biotyping.

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1996-10-01
2024-04-18
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References

  1. Kaplan E. L. The resurgence of group A streptococcal infections and their sequelae. Eur J Clin Microbiol Infect Dis 1991; 10:55–57
    [Google Scholar]
  2. Nielsen S. V., Kolmos H. J. Bacteremia due to different groups of β-haemolytic streptococci: a two-year survey and presentation of a case of recurring infection due to Streptococcus ‘equisimilis’. Infection 1993; 21:358–361
    [Google Scholar]
  3. Skogberg K., Simonen H., Renkonen, O-V, Valtonen W. Beta-haemolytic group A, B, C and G streptococcal septicaemia: a clinical study. Scand J Infect Dis 1988; 20:119–125
    [Google Scholar]
  4. Bamham M., Kerby J., Chandler R. S., Millar M. R. Group C streptococci in human infection: a study of 308 isolates with clinical correlations. Epidemiol Infect 1989; 102:379–390
    [Google Scholar]
  5. Efstratiou A. Outbreaks of human infection caused by pyogenic streptococci of Lancefield groups C and G. J Med Microbiol 1989; 29:207–219
    [Google Scholar]
  6. Mastro T. D., Farley T. A., Elliott J. A. An outbreak of surgical wound infections due to group A Streptococcus carried on the scalp. N Engl J Med 1990; 323:968–972
    [Google Scholar]
  7. Nicolle L. E., Hume K., Sims H., Rosenal T., Sandham D. An outbreak of group A streptococcal bacteremia in an intensive care unit. Infect Control 1986; 7:177–180
    [Google Scholar]
  8. Colman G., Tanna A., Efstratiou A., Gaworzewska E. T. The serotypes of Streptococcus pyogenes present in Britain during 1980-1990 and their association with disease. J Med Microbiol 1993; 39:165–178
    [Google Scholar]
  9. Bouvet A., Geslin P., Kriz-Kuzemenska P., Blanc V., Devine C., Grimont F. Restricted association between biotypes and serotypes within group A streptococci. J Clin Microbiol 1994; 32:1312–1317
    [Google Scholar]
  10. Bert F., Picard B., Lambert-Zechovsky N., Goullet P. Identification and typing of pyogenic streptococci by enzyme electrophoretic polymorphism. J Med Microbiol 1995; 42:442–451
    [Google Scholar]
  11. Musser J. M., Hauser A. R., Kim M. H., Schlievert P. M., Nelson K., Selander R. K. Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression. Proc Natl Acad Sci USA 1991; 88:2668–2672
    [Google Scholar]
  12. Cleary P. P., Kaplan E. L., Livdahl C., Skjold S. DNA fingerprints of Streptococcus pyogenes are M-type specific. J Infect Dis 1988; 158:1317–1323
    [Google Scholar]
  13. Cleary P. P., Kaplan E. L., Handley J. P. Clonal basis for resurgence of serious Streptococcus pyogenes disease in the 1980s. Lancet 1992; 339:518–521
    [Google Scholar]
  14. Seppälä H., Vuopio-Varkila J., Osterblad M. Evaluation of methods for epidemiologic typing of group A streptococci. J Infect Dis 1994; 169:519–525
    [Google Scholar]
  15. Bruneau S., de Montclos H., Drouet E., Denoyel G. A. rRNA gene restriction patterns of Streptococcus pyogenes epidemiological applications and relation to serotypes. J Clin Microbiol 1994; 32:2953–2958
    [Google Scholar]
  16. Single L. A., Martin D. R. Clonal differences within M-types of the group A Streptococcus revealed by pulsed field gel electrophoresis. FEMS Microbiol Lett 1992; 91:85–90
    [Google Scholar]
  17. Barnham M., Cole G., Efstratiou A., Tagg J. R., Skjold S. A. Characterization of Streptococcus zooepidemicus (Lancefield group C) from human and selected animal infections. Epidemiol Infect 1987; 98:171–182
    [Google Scholar]
  18. Galan J. E., Timoney J. F. Immunologic and genetic comparison of Streptococcus equi isolates from the United States and Europe. J Clin Microbiol 1988; 26:1142–1146
    [Google Scholar]
  19. Martin N. J., Kaplan E. L., Gerber M. A. Comparison of epidemic and endemic group G streptococci by restriction enzyme analysis. J Clin Microbiol 1990; 28:1881–1886
    [Google Scholar]
  20. Skjold S. A., Quie P. G., Fries L. A., Bamham M., Cleary P. P. DNA fingerprinting of Streptococcus zooepidemicus (Lancefield group C) as an aid to epidemiological study. J Infect Dis 1987; 155:1145–1150
    [Google Scholar]
  21. Devriese L. A., Hommez J., Kilpper-Balz R., Schleifer K.-H. Streptococcus canis sp. nov: a species of group G streptococci from animals. Int J Syst Bacteriol 1986; 36:422–425
    [Google Scholar]
  22. Farrow J. A. E., Collins M. D. Taxonomic studies on streptococci of serological groups C, G and L and possibly related taxa. Syst Appl Microbiol 1984; 5:483–493
    [Google Scholar]
  23. Kilpper-Bälz R., Schleifer K. H. Nucleic acid hybridization and cell wall composition studies of pyogenic streptococci. FEMS Microbiol Lett 1984; 24:355–364
    [Google Scholar]
  24. Holt J. G., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T. Bergey’s Manual of determinative bacteriology. 9th edn Baltimore; Williams and Wilkins. 1994: 552:
    [Google Scholar]
  25. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990; 18:7213–7218
    [Google Scholar]
  26. Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990; 18:6531–6535
    [Google Scholar]
  27. Van Belkum A. DNA fingerprinting of medically important microorganisms by use of PCR. Clin Microbiol Rev 1994; 7:174–184
    [Google Scholar]
  28. Seppälä H., He Q., Österblad M., Huovinen P. Typing of group A streptococci by random amplified polymorphic DNA analysis. J Clin Microbiol 1994; 32:1945–1948
    [Google Scholar]
  29. Bassam B. J., Caetano-Anollés G., Gresshoff P. M. DNA amplification fingerprinting of bacteria. Appl Microbiol Biotechnol 1992; 38:70–76
    [Google Scholar]
  30. Hunter P. R., Gaston M. A. Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J Clin Microbiol 1988; 26:2465–2466
    [Google Scholar]
  31. Welsh J., Pretzman C., Postic D., Saint Girons I., Baranton G., McClelland M. Genomic fingerprinting by arbitrarily primed polymerase chain reaction resolves Borrelia burgdorferi into three distinct phyletic groups. Int J Syst Bacteriol 1992; 42:370–377
    [Google Scholar]
  32. Ralph D., McClelland M., Welsh J., Baranton G., Perolat P. Leptospira species categorized by arbitrarily primed polymerase chain reaction (PCR) and by mapped restriction polymorphisms in PCR amplified rRNA genes. J Bacteriol 1993; 175:973–981
    [Google Scholar]
  33. Tibayrenc M., Neubauer K., Bamabe C., Guerrini F., Skarecky D., Ayala F.J. Genetic characterization of six parasitic protoza: parity between random-primer DNA typing and multilocus enzyme electrophoresis. Proc Natl Acad Sci USA 1993; 90:1335–1339
    [Google Scholar]
  34. Wang G., Whittman T. S., Berg C. M., Berg D. E. RAPD (arbitrary primer) PCR is more sensitive than multilocus enzyme electrophoresis for distinguishing related bacterial strains. Nucleic Acids Res 1993; 21:5930–5933
    [Google Scholar]
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