%0 Journal Article %A Thakur, Archana %A Prakash, Kunti %T Detection of antibody to C-carbohydrate of group A streptococci with enzyme-treated whole bacterial cells as antigen for ELISA %D 1996 %J Journal of Medical Microbiology, %V 45 %N 3 %P 214-218 %@ 1473-5644 %R https://doi.org/10.1099/00222615-45-3-214 %I Microbiology Society, %X Antibody to specific cell-wall carbohydrate of group A streptococci in human sera was determined by enzyme-linked immunosorbent assay (ELISA) with enzyme-treated whole cells and purified group A carbohydrate (ACHO) as antigens. The optimal concentration of enzyme-treated whole bacterial cells to coat wells was 2 × 108 cells/ml and for purified ACHO antigen the optimal concentration was 1 µ/ml. Sera from patients with acute rheumatic fever and acute glomerulonephritis were screened for the presence of C-carbohydrate antibodies. Patients with acute post-streptococcal complications showed significantly higher titres of antibody when compared with normal healthy individuals. ELISA results were also compared with purified ACHO as an antigen; this showed a highly significant correlation (r = 0.73). Results showed that measurement of the anti-ACHO antibody by ELISA with enzyme-treated whole cells can be a useful, reliable and simple method for serological diagnosis of group A infection and sequelae. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-45-3-214