The effect of and on the adhesion of and to human buccal epithelial cells (BEC) was investigated with a modified membrane filter system. The filters (12 μm diameter pores) acted as a support for the BEC which were pre-exposed to known concentrations of bacterial suspensions (for 45 min-1 h), and then re-incubated with standardised concentrations of yeast suspensions for various periods. The BEC with adherent yeasts were then transferred on to a glass slide, gram-stained and counted by light microscopy. Three of the four bacterial species significantly suppressed adhesion of to BEC; had no effect. Both and suppressed adhesion of to BEC pre-exposed to three different bacterial concentrations, although variable results were obtained with and Significant differences in the relative adhesion of and to BEC were also recorded. These results indicate that the adhesion of yeasts to BEC is modulated both by the composition and the quantity of the pre-existing bacterial flora on the BEC.


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