1887

Abstract

A convenient dipstrip method (Bacteruritest; Mast Diagnostics) for bacterial quantification was evaluated with 42 endotracheal aspirates. For 31 specimens, the dipstrip method yielded counts within a 10-fold range of surface plate counts. Two specimens yielded counts by the dipstrip within a 100-fold range of plate counts. Six specimens yielded confluent growth at the greatest dilution tested by the dipstrip method, and counts > 10 cfu/ml in the surface plate method. Three specimens yielded no detectable growth by the dipstrip and surface plate counts <10 cfu/ml. Dipstrips provide a cheap, convenient method for the routine quantification of the bacterial load in endotracheal aspirates.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-45-2-153
1996-08-01
2022-08-15
Loading full text...

Full text loading...

/deliver/fulltext/jmm/45/2/medmicro-45-2-153.html?itemId=/content/journal/jmm/10.1099/00222615-45-2-153&mimeType=html&fmt=ahah

References

  1. El-Ebiary M., Torres A., González J. Quantitative cultures of endotracheal aspirates for the diagnosis of ventilator-associated pneumonia. Am Rev Respir Dis 1993; 148:1552–1557
    [Google Scholar]
  2. Marquette C. H., Georges H., Wallet F. Diagnostic efficiency of endotracheal aspirates with quantitative bacterial cultures in intubated patients with suspected pneumonia. Comparison with the protected specimen brush. Am Rev Respir Dis 1993; 148:138–144
    [Google Scholar]
  3. Duguid J. P., Collee J. G., Fraser A. G. Laboratory strategy in the diagnosis of infective syndromes. In Collee J. G., Duguid J. P., Fraser A.G., Mannion B. P. (eds) Mackie and McCartney Practical medical microbiology 13th edn Edinburgh: Churchill Living-stone; 1989600–649
    [Google Scholar]
  4. Brown R., Poxton I. R., Wilkinson J. F. Centrifuges, colorimeters and bacterial counts. In Collee J. G., Duguid J. P., Fraser A. G., Mannion B. P. (eds) Mackie and McCartney Practical medical microbiology 13th edn Edinburgh: Churchill Livingstone; 1989200–247
    [Google Scholar]
  5. Leigh D. A., Williams J. D. Method for the detection of significant bacteriuria in large groups of patients. J Clin Pathol 1964; 17:498–503
    [Google Scholar]
  6. Lewis D. A. Bacteriology of urine. In Hawkey P. M., Lewis D. A. (eds) Medical bacteriology: a practical approach Oxford: IRL Press; 19891–19
    [Google Scholar]
  7. Torres A., Martos A., Puig de la Bellacasa J. Specificity of endotracheal aspiration, protected specimen brush, and bronchoalveolar lavage in mechanically ventilated patients. Am Rev Respir Dis 1993; 147:952–957
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-45-2-153
Loading
/content/journal/jmm/10.1099/00222615-45-2-153
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error