1887

Journal of Medical Microbiology logo

Volume 45, Issue 1

Comparison of three molecular methods for typing and subtyping pathogenic strains Free

Abstract

The efficiency of pulsed-field gel electrophoresis (PFGE), ribotyping and restriction enzyme analysis of the virulence plasmid (REAP) for typing and subtyping strains of was compared. All three techniques gave concordant results, and the strains studied could be separated into three distinct clusters: (1) heterogeneous strains of biotype 1A and serotype O5 (1A/O5); (2) one 3/O3 strain and all 2/O9 strains; and (3) all 4/O3, 2/O5 and two 3/O3 strains. Within cluster 3, the 2/O5 and 3/O3 strains were related more closely to each other than to the 4/O3 isolates. With ribotyping, PFGE and phage-typing, the 4/O3 isolates were subdivided into two homogeneous groups, corresponding to strains of phage type IX and strains of other phage types, respectively. Similarly, ribotyping and PFGE subdivided the 2/O9 strains into two conserved groups (I and II), but REAP gave a different subdivision and identified a new REAP pattern (P3). The three techniques confirmed the clear distinction between the heterogeneous group of non-pathogenic 1A/O5 strains and the well conserved group of pathogenic 2/O5 strains. Additional plasmids were identified in some 3/O3 strains. Combined, the results indicated that REAP (with RI) and ribotyping (with RV) are valuable alternatives to bioserotype determination, and PFGE is the most suitable technique for epidemiological tracing.

  • Received:
  • Accepted:
  • Published Online:
© 1996 The Pathological Society of Great Britain and Ireland
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-45-1-48
1996-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/45/1/medmicro-45-1-48.html?itemId=/content/journal/jmm/10.1099/00222615-45-1-48&mimeType=html&fmt=ahah

References

  1. Wauters G., Kandolo K., Janssens M. Revised biogrouping scheme of Yersinia enterocolitica. In Prpic J. K., Davey R. B. (eds) The genus Yersinia: epidemiology, molecular biology and pathogenesis Contrib Microbiol Immunol 1987; 9:14–21
     [Google Scholar]
  2. Fukushima H. Isolation of Yersinia enterocolitica serogroup O:8, biogroup-1B—Reply. J Clin Microbiol 1991; 29:2680
     [Google Scholar]
  3. Wauters G., Aleksic S., Charlier J., Schulze G. Somatic and flagellar antigens of Yersinia enterocolitica and related species. Contrib Microbiol Immunol 1991; 12:239–243
     [Google Scholar]
  4. Nicolle P., Mollaret H. H., Brault J. La lysotypie de Yersinia enterocolitica. Arguments géographiques, zoologiques, antigéniques et biochimiques plaidant en sa faveur. Bull Acad Natl Med 1976; 160:404–108
     [Google Scholar]
  5. Aleksic S., Bockemühl J., Lange F. Studies on the serology of flagellar antigens of Yersinia enterocolitica and related Yersinia species. Zentralbl Bakteriol Mikrobiol Hyg A 1986; 261:299–310
     [Google Scholar]
  6. Najdenski H., Iteman I., Carniel E. Efficient subtyping of pathogenic Yersinia enterocolitica strains by pulsed-field gel electrophoresis. J Clin Microbiol 1994; 32:2913–2920
     [Google Scholar]
  7. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7:1513–1523
     [Google Scholar]
  8. Guiyoule A., Grimont F., Iteman I., Grimont P. A. D., Lefèvre M., Camiel E. Plague pandemics investigated by ribotyping of Yersinia pestis strains. J Clin Microbiol 1994; 32:634–641
     [Google Scholar]
  9. Grimont F., Lèfevre M., Ageron E., Grimont P. A. D. rRNA gene restriction patterns of Legionella species: a molecular identification system. Res Microbiol 1989; 140:615–626
     [Google Scholar]
  10. Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun 1981; 31:775–782
     [Google Scholar]
  11. Heesemann J., Keller C., Morawa R., Schmidt N., Siemens H. J., Laufs R. Plasmids of human strains of Yersinia enterocolitica: molecular relatedness and possible importance for pathogenesis. J Infect Dis 1983; 147:107–115
     [Google Scholar]
  12. Kapperud G., Nesbakken T. Restriction endonuclease analysis of 40- to 50-Mdalton plasmids from Yersinia enterocolitica strains of worldwide origin. Contrib Microbiol Immunol 1987; 9:317–323
     [Google Scholar]
  13. Nesbakken T., Kapperud G., Sørum H., Dommarsnes K. Structural variability of 40-50 Mdal virulence plasmids from Yersinia enterocolitica. Geographical and ecological distribution of plasmid variants. APMIS 1987; 95:167–173
     [Google Scholar]
  14. Kapperud G., Nesbakken T., Aleksic S., Mollaret H. H. Comparison of restriction endonuclease analysis and phenotypic typing methods for differentiation of Yersinia enterocolitica isolates. J Clin Microbiol 1990; 28:1125–1131
     [Google Scholar]
  15. Picard-Pasquie N., Picard B., Heeralal S., Krishnamoorthy R., Goullet P. Correlation between ribosomal DNA polymorphism and electrophoretic enzyme polymorphism in Yersinia. J Gen Microbiol 1990; 136:1655–1666
     [Google Scholar]
  16. Andersen J. K., Saunders N. A. Epidemiological typing of Yersinia enterocolitica by analysis of restriction fragment length polymorphisms with a cloned ribosomal RNA gene. J Med Microbiol 1990; 32:179–187
     [Google Scholar]
  17. Blumberg H. M., Kiehlbauch J. A., Wachsmuth I. K. Molecular epidemiology of Yersinia enterocolitica O:3 infections: use of chromosomal DNA restriction fragment length polymorphisms of rRNA genes. J Clin Microbiol 1991; 29:2368–2374
     [Google Scholar]
  18. Mollaret H. H., Bercovier H., Alonso J. M. Yersinia. In Le Minor L., Véron M. (eds) Bactériologie médicale Paris: Flammarion; 1989451–472
     [Google Scholar]
  19. Pham J. N., Bell S. M., Hardy M. J., Martin L., Guiyoule A., Carniel E. Susceptibility to β-lactam agents of Yersinia enterocolitica biotype 4, serotype O3 isolated in various parts of the world. J Med Microbiol 1995; 43:9–13
     [Google Scholar]
  20. Caugant D. A., Aleksic S., Mollaret H. H., Selander R. K., Kapperud G. Clonal diversity and relationships among strains of Yersinia enterocolitica. J Clin Microbiol 1989; 27:2678–2683
     [Google Scholar]
  21. Comelis G., Laroche Y., Balligand G., Sory, M-P., Wauters G. Yersinia enterocolitica, a primary model for bacterial invasiveness. Rev Infect Dis 1987; 9:64–87
     [Google Scholar]
  22. Fukushima H., Gomyoda M., Aleksic S., Tsubokura M. Differentiation of Yersinia enterocolitica serotype O:5,27 strains by phenotypic and molecular techniques. J Clin Microbiol 1993; 31:1672–1674
     [Google Scholar]
  23. Kaneko S., Maruyama T. Pathogenicity of Yersinia enterocolitica serotype O3 biotype 3 strains. J Clin Microbiol 1987; 25:454–455
     [Google Scholar]
  24. Dolina M., Peduzzi R. Population genetics of human, animal, and environmental Yersinia strains. Appl Environ Microbiol 1993; 59:442–450
     [Google Scholar]
  25. Buchrieser C., Weagant S. D., Kaspar C. W. Molecular characterization of Yersinia enterocolitica by pulsed-field gel electrophoresis and hybridization of DNA fragments to ail and pYV probes. Appl Environ Microbiol 1994; 60:4371–4379
     [Google Scholar]
  26. Saken E., Roggenkamp A., Aleksic S., Heesemann J. Characterisation of pathogenic Yersinia enterocolitica serogroups by pulsed-field gel electrophoresis of genomic Notl restriction fragments. J Med Microbiol 1994; 41:329–338
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-45-1-48
Loading
Comparison of three molecular methods for typing and subtyping pathogenic Yersinia enterocolitica strains
J. Med. Microbiol. 45, 48 (1996); https://doi.org/10.1099/00222615-45-1-48
/content/journal/jmm/10.1099/00222615-45-1-48
/content/journal/jmm/10.1099/00222615-45-1-48
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error