1887

Abstract

Of 28 isolates of non-O1 (10 from diarrhoeal patients and 18 from environmental sources) examined for haemolytic activity and its correlation, if any, with enterotoxic activity, 24 showed haemolysis. The four non-haemolytic isolates showed haemolysis after consecutive passages through rabbit ileal loops (RILs). The titres of haemolytic activity were 4-64 HU/ml irrespective of their source. Eight (28.5%) of the non-O1 isolates caused fluid accumulation; six (25%) were haemolytic and two (50%) non-haemolytic. The remaining isolates showed enterotoxic activity after one-to-three consecutive passages through RILs irrespective of their haemolytic character and source. Environmental isolates caused significantly more fluid accumulation than the diarrhoeal isolates. All these isolates reverted to their original non-toxigenic character on repeated subculture or on storage in the laboratory, but continued to show haemolytic activity. The results of the present study indicate that non-O1 strains are potentially enterotoxigenic independent of their haemolytic character and source, and enterotoxin, not haemolysin, is the factor most likely to be responsible for their enterotoxic activity.

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1996-07-01
2024-03-28
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References

  1. Blake P. A., Weaver R. W., Hollis D. G. Diseases of humans (other than cholera) caused by vibrios. Annu Rev Microbiol 1980; 34:341–367
    [Google Scholar]
  2. Sanyal S. C. NAG Vibrio toxin. In Dorner F., Drews J. (eds) Pharmacology of bacterial toxins. International encyclopedia of pharmacology and therapeutics 119 Oxford: Pergamon Press; 1986207–225
    [Google Scholar]
  3. Gelbart S. M., Prabhudesai M. M. Vibrio cholerae non-O1 cellulitis. Arch Pathol Lab Med 1986; 110:1182–1183
    [Google Scholar]
  4. Hughes J. M., Hollis D. G., Gangarosa E. J., Weaver R. E. Noncholera Vibrio infections in the United States. Clinical, epidemiologic, and laboratory features. Ann Intern Med 1978; 88:602–606
    [Google Scholar]
  5. Safrin S., Morris J. G., Adams M., Pons V., Jacob R., Conte J. E. Non-O:l Vibrio cholerae bacteremia: a case report and review. Rev Infect Dis 1988; 10:1012–1017
    [Google Scholar]
  6. Lahiri A., Agarwal R. K., Sanyal S. C. Biological similarity of enterotoxins of Vibrio cholerae serotypes other than type 1 to cholera toxin and Escherichia coli heat-labile enterotoxin. J Med Microbiol 1982; 15:429–440
    [Google Scholar]
  7. Singh D. V., Tikoo A., Sanyal S. C. Production of the new cholera toxin by environmental isolates by Vibrio cholerae non-O1. J Med Microbiol 1996; 45:31–34
    [Google Scholar]
  8. Arita M., Takeda T., Honda T., Miwatani T. Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin. Infect Immun 1986; 52:45–49
    [Google Scholar]
  9. Honda T., Arita M., Takeda T., Yoh M., Miwatani T. Non-O1 Vibrio cholerae produces two newly identified toxins related to Vibrio parahaemolyticus haemolysin and Escherichia coli heat-stable enterotoxin. Lancet 1985; 2:163–164
    [Google Scholar]
  10. Takao T., Shimonishi Y., Kobayashi M. Amino acid sequence of heat-stable enterotoxin produced by Vibrio cholerae non-O1. FEBS Lett 1985; 193:250–254
    [Google Scholar]
  11. Yoshimura S., Takao T., Shimonishi Y. A heat-stable enterotoxin of Vibrio cholerae non-O1: chemical synthesis, and biological physicochemical properties. Biopolymers 1986; 25: Suppl S69–S83
    [Google Scholar]
  12. Yoh M., Honda T., Miwatani T. Production by non-O1 Vibrio cholerae of haemolysin related to thermostable direct haemolysin of Vibrio parahaemolyticus. FEMS Microbiol Lett 1985; 29:197–200
    [Google Scholar]
  13. O’Brien A. D., Chen M. E., Holmes R. K., Kaper J., Levine M. M. Environmental and human isolates of Vibrio cholerae and Vibrio parahaemolyticus produce a shigella dysenteriae 1 (shiga)-like cytotoxin. Lancet 1984; 1:77–78
    [Google Scholar]
  14. Hanne L. F., Finkelstein R. A. Characterization and distribution of the hemagglutinins produced by Vibrio cholerae. Infect Immun 1982; 36:209–214
    [Google Scholar]
  15. Yamamoto K., Al-Omani M., Honda T., Takeda Y., Miwatani T. Non-O1 Vibrio cholerae hemolysin: purification, partial characterization, and immunological relatedness to E1 Tor hemolysin. Infect Immun 1984; 45:192–196
    [Google Scholar]
  16. Yamamoto K., Ichinose Y., Nakasone N. Identity of hemolysins produced by Vibrio cholerae, non-O1 V. cholerae O1, biotype E1 Tor. Infect Immun 1986; 51:927–931
    [Google Scholar]
  17. Ichinose Y., Yamamoto K., Nakasone N. Enterotoxicity of E1 Tor-like hemolysin of non-O1 Vibrio cholerae. Infect Immun 1987; 55:1090–1093
    [Google Scholar]
  18. Aim R. A., Stroeher U. H., Manning P. A. Extracellular proteins of Vibrio cholerae: nucleotide sequence of the structural gene (hlyA) for the haemolysin of the haemolytic E1 Tor strain of O17 and characterization of the hlyA mutation in the nonhaemolytic classical strain 569B. Mol Microbiol 1988; 2:481–488
    [Google Scholar]
  19. Tikoo A., Singh D. V., Sanyal S. C. Influence of animal passage on haemolysin and enterotoxin production in Vibrio cholerae O1 biotype E1 Tor strains. J Med Microbiol 1994; 40:246–251
    [Google Scholar]
  20. World Health Organization Programme for control of diarrhoeal diseases (CDD/83.3 Rev. 1). In Manual for laboratory investigations of acute enteric infections Geneva: World Health Organization; 1987
    [Google Scholar]
  21. Richardson K., Michalski J., Kaper J. B. Hemolysin production and cloning of two hemolysin determinants from classical Vibrio cholerae. Infect Immun 1986; 54:415–420
    [Google Scholar]
  22. Iwanaga M., Yamamoto K., Higa N., Ichinose Y., Nakasone N., Tanabe M. Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 E1 Tor. Microbiol Immunol 1986; 30:1075–1083
    [Google Scholar]
  23. Smith H. W. Haemolysins of Escherichia coli. J Pathol Bacteriol 1963; 85:197–211
    [Google Scholar]
  24. Rennie R. P., Arbuthnott J. P. Partial characterisation of Escherichia coli haemolysin. J Med Microbiol 1974; 7:179–188
    [Google Scholar]
  25. Singh D. V., Sanyal S. C. Production of haemolysis and its correlation with enterotoxicity in Aeromonas spp. J Med Microbiol 1992; 37:262–267
    [Google Scholar]
  26. De S. N., Chatterje D. N. Experimental study of the mechanism of action of Vibrio cholerae on the intestinal mucous membrane. J Pathol Bacteriol 1953; 66:559–562
    [Google Scholar]
  27. Sanyal S. C., Huq M. I., Neogy P. K. B., Alam K., Kabir M. I., Rahaman A. S. M. H. Experimental studies on the pathogenicity of Vibrio mimicus strains isolated in Bangladesh. Aust J Exp Bio Med Sci 1984; 62:515–521
    [Google Scholar]
  28. Sanyal S. C., Neogy P. K. B., Alam K., Huq M. I., Al-Mahmud K. A. A new enterotoxin produced by Vibrio cholerae O1. J Diarrhoeal Dis Res 1984; 2:3–12
    [Google Scholar]
  29. Brown M. H., Manning P. A. Haemolysin gene of Vibrio cholerae: presence of homologous DNA in non-haemolytic O1 and haemolytic non O1 strains. FEMS Microbiol Lett 1985; 30:197–201
    [Google Scholar]
  30. Yamamoto K., Ichinose Y., Shinagawa H. Two-step processing for activation of the cytolysin/hemolysin of Vibrio cholerae O1 biotype E1 Tor: nucleotide sequence of the structural gene (hlyA) and characterization of the processed products. Infect Immun 1990; 58:4106–4116
    [Google Scholar]
  31. Singh D. V., Sanyal S. C. Production of chitinase by enterotoxigenic Aeromonas species isolated from clinical and environmental sources. J Diarrhoeal Dis Res 1992; 10:213–216
    [Google Scholar]
  32. Shankar P., Agarwal R. K., Sanyal S. C. Experimental studies on enteropathogenicity of Vibrio cholerae serotypes other than O1. Zentralbl Bakteriol Mikrobiol Hyg A 1982; 252:514–524
    [Google Scholar]
  33. Sanyal S. C., Agarwal R. K., Annapurna E., Lee J. V. Enterotoxicity of group F vibrios. Jpn J Med Sci Biol 1980; 33:217–222
    [Google Scholar]
  34. Sanyal S. C., Huq M. I., Neogy P. K. B. Vibrio mimicus as an aeteologic agent of diarrhoea and its pathogenesis. Indian J Med Microbiol 1983; 1:1–12
    [Google Scholar]
  35. Annapurna E., Sanyal S. C. Studies on enteropathogenicity of Aeromonas hydrophila in an experimental model. Indian J Prev Soc Med 1975; 6:234–237
    [Google Scholar]
  36. Annapurna E., Sanyal S. C. Enterotoxicity of Aeromonas hydrophila. J Med Microbiol 1977; 10:317–323
    [Google Scholar]
  37. Singh D. V., Sanyal S. C. Haemolysin and enterotoxin production by Aeromonas caviae isolated from diarrhoeal patients, fish and environment. J Diarrhoeal Dis Res 1992; 10:16–20
    [Google Scholar]
  38. Singh D. V., Sanyal S. C. Enterotoxicity of clinical and environmental isolates of Aeromonas spp. J Med Microbiol 1992; 36:269–272
    [Google Scholar]
  39. Sanyal S. C., Saraswathi B., Sharma P. Enteropathogenicity of Plesiomonas shigelloides. J Med Microbiol 1980; 13:401–409
    [Google Scholar]
  40. Raychaudhury A., Agarwal R. K., Sanyal S. C. Enteropathogenicity of Klebsiella pneumoniae strains isolated from stools of diarrhoeal patients and other clinical specimens: an experimental study. Zentralbl Bakteriol Mikrobiol Hyg A 1984; 258:94–103
    [Google Scholar]
  41. Singh S. J., Sanyal S. C. Enterotoxicity of so-called NAG vibrios. Ann Soc Belg Med Trop 1978; 58:133–140
    [Google Scholar]
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