1887

Abstract

Different PCR-based DNA fingerprinting techniques were evaluated for typing 26 clinical isolates belonging to the complex. Seven isolates belonged to a previously defined outbreak while 19 isolates were unrelated epidemiologically. The PCR-based DNA fingerprinting techniques used were: (i) repetitive extragenic palindromic (REP) PCR; (ii) enterobacterial repetitive intergenic consensus (ERIC) PCR; (iii) randomly amplified polymorphic DNA with M13 forward primer; (iv) restriction analysis of the amplified 16S rRNA gene (ARDRA-16S); and (v) restriction analysis of an amplified region containing the 16S-23S rRNA spacer region and part of the 23S rRNA gene (ARDRA 23S + spacer). The discrimination index for the PCR-based DNA fingerprinting techniques was: 0.99 for REP; 0.94 for ERIC; 0.87 for M13; 0.60 for ARDRA-16S digested with II and <0.50 for ARDRA 23S + spacer. It was concluded that REP-PCR possessed high discriminatory power and reproducibility in comparison with the other PCR-based DNA fingerprinting techniques, and is a simple and rapid typing method for use in epidemiological studies of isolates belonging to the complex.

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1996-06-01
2021-12-01
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References

  1. Beck-Sagué C. M., Jarvis W. R., Brook J. H. Epidemic bacteremia due to Acinetobacter baumannii in five intensive care units. Am J Epidemiol 1990; 132:723–733
    [Google Scholar]
  2. Castle M., Tenney J. H., Weinstein M. P., Eickhoff T. C. Outbreak of a multiply resistant Acinetobacter in surgical intensive care unit: epidemiology and control. Heart Lung 1978; 7:641–644
    [Google Scholar]
  3. French G. L., Casewell M. W., Roncoroni A. J., Knight S., Phillips I. A hospital outbreak of antibiotic-resistant Acinetobacter anitratus: epidemiology and control. J Hosp Infect 1980; 1:125–131
    [Google Scholar]
  4. Hartstein A. I., Morthland V. H., Rourke J. W. Plasmid DNA fingerprinting of Acinetobacter calcoaceticus subspecies anitratus from intubated and mechanically ventilated patients. Infect Control Hosp Epidemiol 1990; 11:531–538
    [Google Scholar]
  5. Marcos M. A., Abdalla S., Pedraza F. Epidemiological markers of Acinetobacter baumannii clinical isolates from a Spinal Cord Injury Unit. J Hosp Infect 1994; 28:39–48
    [Google Scholar]
  6. Vila J., Almela M., Jimenez de Anta M. T. Laboratory investigation of hospital outbreak caused by two different multiresistant Acinetobacter calcoaceticus subsp. anitratus strains. J Clin Microbiol 1989; 27:1086–1089
    [Google Scholar]
  7. Allen K. D., Green H. T. Hospital outbreak of multi-resistant Acinetobacter anitratus: an airborne mode of spread?. J Hosp Infect 1987; 9:110–119
    [Google Scholar]
  8. Bouvet P. J. M., Jeanjean S., Vieu, J-F., Dijkshoom L. Species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains. J Clin Microbiol 1990; 28:170–176
    [Google Scholar]
  9. Kropec A., Hubner J., Daschner F. D. Comparison of three typing methods in hospital outbreaks of Acinetobacter calcoaceticus infection. J Hosp Infect 1993; 23:133–142
    [Google Scholar]
  10. Traub W. H. Acinetobacter baumannii serotyping for delineation of outbreaks of nosocomial cross-infection. J Clin Microbiol 1989; 27:2713–2716
    [Google Scholar]
  11. Alexander M., Ismail F., Jackson P. J. H., Noble W. C. Fingerprinting Acinetobacter strains from clinical sources by numerical analysis of electrophoretic protein patterns. J Med Microbiol 1984; 18:55–64
    [Google Scholar]
  12. Vila J., Canales M. A., Marcos M. A., Gomez-Lus R., Jimenez de Anta M. T. Molecular epidemiological analysis of nosocomial Acinetobacter baumannii isolates. In Towner K. J., Bergogne-Bérézin E., Fewson C. A. (eds) The biology of Acinetobacter. Taxonomy, clinical importance, molecular biology, physiology, industrial relevance New York: Plenum Press; 199169–76
    [Google Scholar]
  13. Dijkshoom L., Van Vianen W., Degener J. E., Michel M. F. Typing of Acinetobacter calcoaceticus strains isolated from hospital patients by cell envelope protein profiles. Epidemiol Infect 1987; 99:659–667
    [Google Scholar]
  14. Dijkshoom L., Michel M. F., Degener J. E. Cell envelope protein profiles of Acinetobacter calcoaceticus strains isolated in hospitals. J Med Microbiol 1987; 23:313–319
    [Google Scholar]
  15. Gemer-Smidt P. Ribotyping of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. J Clin Microbiol 1992; 30:2680–2685
    [Google Scholar]
  16. Allardet-Servent A., Bouziges N., Carles-Nurit, M-J., Bourg G., Gouby A., Ramuz M. Use of low-frequency-cleavage restriction endonucleases for DNA analysis epidemiological investigations of nosocomial bacterial infections. J Clin Microbiol 1989; 27:2057–2061
    [Google Scholar]
  17. Marcos M. A., Jimenez de Anta M. T., Vila J. Correlation of six methods for typing nosocomial isolates of Acinetobacter baumannii. J Med Microbiol 1995; 42:328–335
    [Google Scholar]
  18. Lupski J. R. Molecular mechanisms for transposition of drug-resistance genes and other movable genetic elements. Rev Infect Dis 1987; 9:357–368
    [Google Scholar]
  19. Menard C., Brousseau R., Mouton C. Application of polymerase chain reaction with arbitrary primer (AP-PCR) to strain identification of Porphyromonas (Bacteroides) gingivalis. FEMS Microbiol Lett 1992; 95:163–168
    [Google Scholar]
  20. Niesters H. G. M., Goessens W. H. F., Meis J. F. M. G., Quint W. G. V Rapid polymerase chain reaction-based identification assays for Candida species. J Clin Microbiol 1993; 31:904–910
    [Google Scholar]
  21. Struelens M. J., Bax R., Deplano A., Quint W., G V., Van Belkum A. Concordant clonal delineation of methicillin-resistant Staphylococcus aureus by macro restriction analysis and polymerase chain reaction genome fingerprinting. J Clin Microbiol 1993; 31:1964–1970
    [Google Scholar]
  22. Gräser Y., Klare I., Halle E. Epidemiological study of an Acinetobacter baumannii outbreak by using polymerase chain reaction fingerprinting. J Clin Microbiol 1993; 31:2417–2420
    [Google Scholar]
  23. Vila J., Marcos A., Llovet T., Coll P., Jimenez de Anta T. A comparative study of ribotyping and arbitrarily primed polymerase chain reaction for investigation of hospital outbreaks of Acinetobacter baumannii infection. J Med Microbiol 1994; 41:244–249
    [Google Scholar]
  24. Struelens M. J., Carlier E., Maes N., Serruys E., Quint W. G., Van Belkum A. Nosocomial colonization and infection with multi-resistant Acinetobacter baumannii: outbreak delineation using DNA macrorestriction analysis and PCR-fingerprinting. J Hosp Infect 1993; 25:15–32
    [Google Scholar]
  25. Reboli A. C., Houston E. D., Monteforte J. S., Wood C. A., Hamill R. J. Discrimination of epidemic and sporadic isolates of Acinetobacter baumannii by repetitive element PCR-mediated DNA fingerprinting. J Clin Microbiol 1994; 32:2635–2640
    [Google Scholar]
  26. Bouvet P. J. M., Grimont P. A. D. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov., and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter Iwoffii. Int J Syst Bacteriol 1986; 36:228–240
    [Google Scholar]
  27. Marcos M. A., Vila J., Jimenez de Anta M. T. Epidemiologia de las infecciones de Acintobacter baumannii. Enfer Infec Microbiol Clin 1993; 11:29–33
    [Google Scholar]
  28. Wachsmuth K. Molecular epidemiology of bacterial infections: examples of methodology and of investigations of outbreaks. Rev Infect Dis 1986; 8:682–692
    [Google Scholar]
  29. Versalovic J., Koeuth T., Lupski J. R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 1991; 19:6823–6831
    [Google Scholar]
  30. Van Belkum A., Bax R., Peerbooms P., Goessens W. H. F., Van Leeuwen N., Quint W. G. V. Comparison of phage typing and DNA fingerprinting by polymerase chain reaction for discrimination of methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol 1993; 31:798–803
    [Google Scholar]
  31. Versalovic J., Kapur Y., Mason E. O. Penicillin-resistant Streptococcus pneumoniae strains recovered in Houston: identification and molecular characterization of multiple clones. J Infect Dis 1993; 167:850–856
    [Google Scholar]
  32. Gurtler V., Wilson V. A., Mayall B. C. Classification of medically important clostridia using restriction endonuclease site differences of PCR-amplified 16S rDNA. J Gen Microbiol 1991; 137:2673–2679
    [Google Scholar]
  33. Vaneechoutte M., Dijkshoom L., Tjemberg I. Identification of Acinetobacter genomic species by amplified ribosomal DNA restriction analysis. J Clin Microbiol 1995; 33:11–15
    [Google Scholar]
  34. Barry T., Colleran G., Glennon M., Dunican L. K., Gannon F. The 16S/23S ribosomal spacer region as a target for DNA probes to identify eubacteria. PCR Methods Applic 1991; 1:51–56
    [Google Scholar]
  35. Matar G. M., Swaminathan B., Hunter S. B., Slater L. N., Welch D. F. Polymerase chain reaction-based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from Rochalima species for subtyping. J Clin Microbiol 1993; 31:1730–1734
    [Google Scholar]
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