Escherichia coli MT78, an avian pathogenic strain of serogroup O2, produces a variant form of type 1 fimbriae with distinct antigenic properties and apparent mol. wt of the major subunit. The fim gene cluster of strain MT78 was cloned and its sequence was determined in a region spanning upstream of fimB to the beginning of fimD. Whereas most genes were well conserved relative to fim genes previously described, comparison of the fimA gene from strain MT78 with homologous sequences from other strains of E. coli and Klebsiella pneumoniae revealed that most differences were clustered in four well defined regions. A PCR assay, based upon these variable sequences, allowed amplification of a fragment of gene fimA which is specific for most O2 strains. In addition, the sequence of the previously uncharacterised gene fimI, which is located between genes fimA and fimC, was determined.
KrogfeltK. A. Bacterial adhesion: genetics, biogenesis, and role in pathogenesis of fimbrial adhesins of Escherichia coli. Rev Infect Dis1991; 13:721–735
GrossW. B. Colibacillosis. In
CalnekB. W.,
BarnesH. J.,
BeardC. W.,
ReidW. M.,
YoderH. W.
(eds) Diseases of poultry 9th edn Ames, Iowa: Iowa State University Press; 1991138–144
DozoisC. M.,
ChanteloupN.,
Dho-MoulinM.,
BréeA.,
DesautelsC.,
FairbrotherJ. M. Bacterial colonization and in vivo expression of FI (type 1) fimbrial antigens in chickens experimentally infected with pathogenic Escherichia coli. Avian Dis1994; 38:231–239
GyimahJ. E.,
PanigrahyB. Adhesin-receptor interactions mediating the attachment of pathogenic Escherichia coli to chicken tracheal epithelium. Avian Dis1988; 32:74–78
SuwanichkulA.,
PanigrahyB.,
WagnerR. M. Antigenic relatedness and partial amino acid sequences of pili of Escherichia coli serotypes 01, 02, and 078 pathogenic to poultry. Avian Dis1987; 31:809–813
SekizakiT.,
ItoH.,
AsawaT.,
NonomuraI. DNA sequence of type 1 fimbrin, Fpull, gene from a chicken pathogenic Escherichia coli serotype O78. J Vet Med Sci1993; 3:395–400
Dho-MoulinM.,
van den BoschJ. F.,
GirardeauJ. P.,
BreeA.,
LafontJ. P. Surface antigens from Escherichia coli 02 and 078 strains of avian origin. Infect Immun1990; 58:740–745
KlemmP. The fimA gene encoding the type-1 fimbrial subunit of Escherichia coli. Nucleotide sequence and primary structure of protein. Eur J Biochem1984; 143:395–399
BoyerH. W.,
Roulland-DussoixD. A. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol1969; 41:459–472
BlomfieldI. C.,
McClainM. S.,
EisensteinB. I. Type 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutants. Mol Microbiol1991; 5:1439–1445
HullR. A.,
GillR. E.,
HsuP.,
MinshewB. H.,
FalkowS. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infect Immun1981; 33:933–938
KlemmP.,
ChristiansenG. Three fim genes required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. Mol Gen Genet1987; 208:439–445
JonesC. H.,
PinknerJ. S.,
NicholesA. V.,
SlonimL. N.,
AbrahamS. N.,
HultgrenS. J. FimC is a periplasmic PapD-like chaperone that directs assembly of type 1 pili in bacteria. Proc Natl Acad Sci USA1993; 90:8397–8401
RossoliniG. M.,
MuscasP.,
ChiesurinA.,
SattaG. Analysis of the Salmonella Jim gene cluster: identification of a new gene (fiml) encoding a fimbrin-like protein and located downstream from the fimA gene. FEMS Microbiol Lett1993; 114:259–266
RiegmanN.,
KustersR.,
van VeggelH. F1C fimbriae of a uropathogenic Escherichia coli strain: genetic and functional organization of the foe gene cluster and identification of minor subunits. J Bacteriol1990; 172:1114–1120
van DieI.,
van GefifenB.,
HoekstraW.,
BergmansH. Type 1C fimbriae of a uropathogenic Escherichia coli strain: cloning and characterization of the genes involved in the expression of the 1C antigen and nucleotide sequence of the subunit gene. Gene1985; 34:187–196
PurcellB. K.,
PrucklerJ.,
CleggS. Nucleotide sequences of the genes encoding type 1 fimbrial subunits of Klebsiella pneumoniae and Salmonella typhimurium. J Bacteriol1987; 169:5831–5834
OmdorfF. P. E.,
FalkowS. Nucleotide sequence of pilA, the gene encoding the structural component of type 1 pili in Escherichia coli. J Bacteriol1985; 162:454–457
PereA.,
Väisänen-RhenV.,
RhenM.,
TenhunenJ.,
KorhonenT. K. Analysis of P fimbriae on Escherichia coli O2, O4, and O6 strains by immunoprecipitation. Inject Immun1986; 51:618–625
KlemmP.,
JørgensenB. J.,
KreftB.,
ChristiansenG. The export systems of type 1 and F1C fimbriae are interchangeable but work in parental pairs. J Bacteriol1995; 177:621–627
BägaM.,
NorgrenM.,
NormarkS. Biogenesis of E. coli Pap pili: PapH, a minor pilin subunit involved in cell anchoring and length modulation. Cell1987; 49:241–251
MaitiS. N.,
DesGroseillersL.,
FairbrotherJ. M.,
HarelJ. Analysis of genes coding for the major and minor fimbrial subunits of the Prs-like fimbriae F16511 of porcine septicemic Escherichia coli strain 4787. Microb Pathog1994; 16:15–25
WhiteD. G.,
Dho-MoulinM.,
WilsonR. A.,
WhittamT. S. Clonal relationships and variation in virulence among Escherichia coli strains of avian origin. Microb Pathog1993; 14:399–409
SokurenkoE. V.,
CourtneyH. S.,
OhmanD. E.,
KlemmP.,
HastyD. L. FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among JimH genes. J Bacteriol1994; 176:748–755
SokurenkoE. V.,
CourtneyH. S.,
MaslowJ.,
SiitonenA.,
HastyD. L. Quantitative differences in adhesiveness of type 1 fimbriated Escherichia coli due to structural differences in JimH genes. J Bacteriol1995; 177:3680–3686