1887

Abstract

The relationships between isolates suggested by a novel DNA typing method (RAPD-ALFA) that combines randomly amplified polymorphic DNA with automated on-line laser fluorescence analysis of DNA fragments were compared with those suggested by four other computer-assisted typing strategies (biotyping, antibiogram typing, pulsed-field gel analysis of chromosomal fingerprints and arbitrarily-primed DNA amplification with three different primers) for 25 isolates of obtained from 12 different hospitals in four countries over a period of 12 years. The results obtained by cluster analysis with two different software packages confirmed that the relationships suggested by RAPD-ALFA were robust and essentially similar to those suggested by the other more laborious computer-assisted typing methods. The technique of RAPD-ALFA appears to offer the possibility of routine on-line molecular identification and typing of isolates from particular hospital wards or units (e.g., intensive care units), and could, therefore, play a key role in the early recognition and prevention of outbreaks of infection.

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1996-03-01
2024-06-16
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References

  1. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990; 18:7213–7218
    [Google Scholar]
  2. Maslow J. N., Mulligan M. E., Arbeit R. D. Molecular epidemiology: application of contemporary techniques to the typing of microorganisms. Clin Infect Dis 1993; 17:153–164
    [Google Scholar]
  3. Caetano-Anollés G., Bassam B. J., Gresshoff P. M. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Biotechnology 1991; 9:553–557
    [Google Scholar]
  4. Meunier J. R., Grimont P. A. D. Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res Microbiol 1993; 144:373–379
    [Google Scholar]
  5. Towner K. J., Cockayne A. Molecular methods for microbial identification and typing. London: Chapman & Hall; 1993
    [Google Scholar]
  6. Arbeit R. D., Maslow J. N., Mulligan M. E. Polymerase chain reaction-mediated genotyping in microbial epidemiology: reply. Clin Infect Dis 1994; 18:1018–1019
    [Google Scholar]
  7. Grundmann H., Schneider, Ch., Tichy H. V. Automated laser fluorescence analysis of randomly amplified polymorphic DNA: a rapid method for investigating nosocomial transmission of Acinetobacter baumannii . J Med Microbiol 1995; 43:446–451
    [Google Scholar]
  8. Voss H., Stegemann J., Schwager C. High-speed automated DNA fragment analysis for genome mapping by restriction fingerprinting. Methods Mol Cell Biol 1992; 3:77–82
    [Google Scholar]
  9. Bergogne-Bérézin E., Joly-Guillou M. L., Towner K. J. (eds) Acinetobacter. microbiology, epidemiology, infections, management Boca Raton: CRC Press; 1995
    [Google Scholar]
  10. Crowe M., Towner K. J., Humphreys H. Clinical and epidemiological features of an outbreak of acinetobacter infection in an intensive therapy unit. J Med Microbiol 1995; 43:55–62
    [Google Scholar]
  11. Bergogne-Bérézin E., Joly-Guillou M. L., Vieu J. F. Epidemiology of nosocomial infections due to Acinetobacter calcoaceticus . J Hosp Infect 1987; 10:105–113
    [Google Scholar]
  12. Noble W. C. Hospital epidemiology of Acinetobacter infection. In Towner K. J., Bergogne-Berezin E., Fewson C. A. (eds) The biology of Acinetobacter: taxonomy, clinical importance, molecular biology, physiology, industrial relevance New York: Plenum Press; 199153–62
    [Google Scholar]
  13. Wiedmannal-Ahmad M., Tichy H. V., Schön G. Characterization of Acinetobacter type strains and isolates obtained from wastewater treatment plants by PCR fingerprinting. Appl Environ Microbiol 1994; 60:4066–4071
    [Google Scholar]
  14. Bouvet P. J. M., Grimont P. A. D. Identification and biotyping of clinical isolates of Acinetobacter . Ann Inst Pasteur Microbiol 1987; 138:569–578
    [Google Scholar]
  15. Kämpfer P., Tjemberg I., Ursing J. Numerical classification and identification of Acinetobacter genomic species. J Appl Bacteriol 1993; 75:259–268
    [Google Scholar]
  16. Horrevorts A., Bergman K., Kollée L., Breuker I., Tjemberg I., Dijkshoom L. Clinical and epidemiological investigations of Acinetobacter genomospecies 3 in a neonatal intensive care unit. J Clin Microbiol 1995; 33:1567–1572
    [Google Scholar]
  17. Curran R., Hardie K. R., Towner K. J. Analysis by pulsed-field gel electrophoresis of insertion mutations in the transferrin-binding system of Haemophilus influenzae type b. J Med Microbiol 1994; 41:120–126
    [Google Scholar]
  18. Vila J., Marcos A., Llovet T., Coll P., Jimenez de Anta T. A comparative study of ribotyping and arbitrarily primed polymerase chain reaction for investigation of hospital outbreaks of Acinetobacter baumannii infection. J Med Microbiol 1994; 41:244–249
    [Google Scholar]
  19. Barbut F., Mario N., Delmeé, M., Gozian J., Petit J. -C. Genomic fingerprinting of Clostridium difficile isolates by using a random amplified polymorphic DNA (RAPD) assay. FEMS Microbiol Lett 1993; 114:161–166
    [Google Scholar]
  20. Dijkshoom L. Acinetobacter – microbiology. In Bergogne-Bérézin E., Joly-Guillou M. L., Towner K. J. (eds) Acinetobacter: microbiology, epidemiology, infections, management Boca Raton: CRC Press; 199537–69
    [Google Scholar]
  21. Dijkshoom L., Aucken H. M., Gemer-Smidt P., Kaufinann M. E., Ursing J., Pitt T. L. Correlation of typing methods for Acinetobacter isolates from hospital outbreaks. J Clin Microbiol 1993; 31:702–705
    [Google Scholar]
  22. Weemink A., Severin W. P. J., Tjemberg I., Dijkshoom L. Pillows, an unexpected source of Acinetobacter . J Hosp Infect 1995; 29:189–199
    [Google Scholar]
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